Longitudinal Study of Vaginal Microbiota and Persistent Human Papillomavirus Detection

阴道微生物群和持续人乳头瘤病毒检测的纵向研究

• 批准号: 9232554
• 负责人: Purnima Madhivanan
• 金额: $ 43.95万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-07 至 2021-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9232554
• 关键词: African American; Anaerobic Bacteria; Bacteria; Bacterial Vaginosis; Bypass; Cervical Intraepithelial Neoplasia; Cervical dysplasia; Cessation of life; Chlamydia Infections; Cities; Clinical Trials; Complement; Data; Detection; Development; Diagnosis; Dimensions; Education; Engineering; Epithelial Cells; Epithelium; Ethnic Origin; Florida; Genes; Genital Human Papilloma Virus Infection; Gonorrhea; Grant; Guidelines; Health; Human Papilloma Virus Vaccination; Human Papilloma Virus Vaccine; Human Papillomavirus; Human papilloma virus infection; Incidence; Infection; Institution; International; Intervention; Investments; Lactic acid; Lactobacillus; Latina; Longitudinal Studies; Malignant neoplasm of cervix uteri; Medicine; Metronidazole; Mexican Americans; Minority; Mucous body substance; National Cancer Institute; National Institute of Allergy and Infectious Disease; Oncogenic; Pilot Projects; Play; Population; Prevention; Public Health; Randomized; Reproductive Health; Research; Research Infrastructure; Research Priority; Ribosomal RNA; Risk; Risk Factors; Role; Sampling; Screening for cancer; Social Work; Students; Swab; System; Target Populations; Time; Triage; United States National Institutes of Health; Universities; Vaccine Research; Vaccines; Vagina; Woman; cancer prevention; college; computer science; graduate student; health disparity; high risk; infrastructure development; innovation; longitudinal analysis; microbiota; mortality; open label; prophylactic; pyrosequencing; racial and ethnic disparities; research and development; screening; undergraduate student; vaginal microbiome

Project Summary/Abstract Title: Longitudinal Study of Vaginal Microbiota and Persistent Human Papillomavirus Detection Abstract: In the US, there are about 7 million new genital human papillomavirus (HPV) infections with high-risk oncogenic HPV types each year. About 10% of these, or 700,000 are persistent; this is an important risk factor for cervical dysplasia and invasive cervical cancer. The dynamics of HPV infection and the subsequent development of high-grade cervical intraepithelial neoplasia are not well understood. The proposed study will characterize the temporal relationship of vaginal microbiota associated with Metronidazole treatment of asymptomatic bacterial vaginosis (BV). It will also examine whether Metronidazole treatment of asymptomatic BV is associated with detection of oncogenic HPV and persistence of oncogenic HPV infections. The proposed pilot study will utilize existing vaginal swabs from 80 women followed over six months from the BRAVO study, a randomized controlled open labeled clinical trial examining Metronidazole treatment of asymptomatic BV. The vaginal microbiome will be characterized using high throughput 16s rRNA encoding gene pyrosequencing. The proposed research fits with priorities of the National Cancer Institute (NCI) and National Institute of Allergy and Infectious Disease (NIAID). NCI has prioritized racial and ethnic disparities in the incidence and mortality of certain cancers and prevention or early detection of these cancers in specific populations and NIAID has set “screening, diagnosis and interventions for target populations to determine how to triage various groups for appropriate prevention, treatment and education strategies” as an overarching priority. There are also compelling public health reasons for supporting this application: In spite of the availability of prophylactic HPV vaccination, there are still more than 528,000 new cases and 266,000 deaths worldwide from cervical cancer related to persistent HPV infections each year. This innovative research will complement current HPV vaccine research by providing more data on how the dysbiosis associated with BV allows HPV to bypass vaginal defenses to infect basal epithelial cells—providing information that could contribute to more effective vaccines. It also may help inform treatment guidelines. Finally, it will build research infrastructure and opportunities for student involvement at the largest minority majority institution in the U.S.

项目摘要/摘要 标题：阴道微生物区系与持续性人乳头瘤病毒检测的纵向研究 摘要：在美国，大约有700万新的生殖器人类乳头瘤病毒(HPV)感染与 每年高危致癌HPV类型。其中约10%，即70万人是持久的；这是一个 宫颈不典型增生和浸润性宫颈癌的重要危险因素。人乳头瘤病毒感染的动态和 高级别宫颈上皮内瘤变的后续发展尚不清楚。这个 拟议的研究将描述与甲硝唑相关的阴道微生物区系的时间关系 治疗无症状细菌性阴道病(BV)。它还将审查甲硝唑是否治疗 无症状BV与致癌性HPV的检测和致癌性HPV感染的持久性有关。 这项拟议的初步研究将利用现有的80名妇女的阴道拭子，对其进行为期6个月的跟踪调查。 来自Bravo研究，一项检查甲硝唑治疗的随机对照开放标签临床试验 无症状的BV。阴道微生物组将使用高通量16S rRNA编码来表征 基因焦磷酸测序。这项拟议的研究符合美国国家癌症研究所(NCI)和 美国国家过敏和传染病研究所(NIAID)。NCI将种族和民族差异放在了优先地位 某些癌症的发病率和死亡率以及对这些癌症的预防或早期发现 人口和NIAID已经设定了“对目标人群进行筛查、诊断和干预以确定如何 对不同群体进行分类，以便采取适当的预防、治疗和教育战略“，作为首要任务 优先考虑。支持这一应用程序也有令人信服的公共卫生原因：尽管 获得预防性HPV疫苗接种后，仍有528,000多例新病例和266,000例死亡 世界各地每年因持续的HPV感染而患上宫颈癌。这项创新的研究将 补充目前的HPV疫苗研究，提供更多关于BV的生物失调如何关联的数据 允许HPV绕过阴道防御感染基底上皮细胞--提供的信息可能 为更有效的疫苗做出贡献。它还可能有助于为治疗指南提供信息。最后，它将建立研究 基础设施和学生在美国最大的少数族裔占多数的学校的参与机会

项目成果

HIV risk and testing behaviors among pregnant women tested for HIV in Florida by site type, 2012.

2012 年佛罗里达州按地点类型进行 HIV 检测的孕妇的 HIV 风险和检测行为。

• DOI: 10.1080/03630242.2019.1565902
• 发表时间: 2019
• 期刊: Women & health
• 影响因子: 1.6
• 作者: Taveras,Janelle;Trepka,MaryJo;Madhivanan,Purnima;Gollub,EricaL;Dévieux,JessyG;Ibrahimou,Boubakari
• 通讯作者: Ibrahimou,Boubakari

Acoustic regularities in infant-directed speech and song across cultures.

• DOI: 10.1038/s41562-022-01410-x
• 发表时间: 2022-11
• 期刊: NATURE HUMAN BEHAVIOUR
• 影响因子: 29.9
• 作者: Hilton, Courtney B.;Moser, Cody J.;Bertolo, Mila;Lee-Rubin, Harry;Amir, Dorsa;Bainbridge, Constance M.;Simson, Jan;Knox, Dean;Glowacki, Luke;Alemu, Elias;Galbarczyk, Andrzej;Jasienska, Grazyna;Ross, Cody T.;Neff, Mary Beth;Martin, Alia;Cirelli, Laura K.;Trehub, Sandra E.;Song, Jinqi;Kim, Minju;Schachner, Adena;Vardy, Tom A.;Atkinson, Quentin D.;Salenius, Amanda;Andelin, Jannik;Antfolk, Jan;Madhivanan, Purnima;Siddaiah, Anand;Placek, Caitlyn D.;Salali, Gul Deniz;Keestra, Sarai;Singh, Manvir;Collins, Scott A.;Patton, John Q.;Scaff, Camila;Stieglitz, Jonathan;Cutipa, Silvia Ccari;Moya, Cristina;Sagar, Rohan R.;Anyawire, Mariamu;Mabulla, Audax;Wood, Brian M.;Krasnow, Max M.;Mehr, Samuel A.
• 通讯作者: Mehr, Samuel A.

Does Zika Virus Lead to Microcephaly in Organoids?

寨卡病毒会导致类器官小头畸形吗？

• DOI: 10.1093/infdis/jiz215
• 发表时间: 2019
• 期刊: The Journal of infectious diseases
• 作者: Coudray,Makella;Kiplagat,Sandra;Madhivanan,Purnima
• 通讯作者: Madhivanan,Purnima

Dynamic Bayesian Networks for Integrating Multi-omics Time Series Microbiome Data.

• DOI: 10.1128/msystems.01105-20
• 发表时间: 2021-03-30
• 期刊: mSystems
• 影响因子: 6.4
• 作者: Ruiz-Perez D;Lugo-Martinez J;Bourguignon N;Mathee K;Lerner B;Bar-Joseph Z;Narasimhan G
• 通讯作者: Narasimhan G

Factors Associated With the Recurrence, Persistence, and Clearance of Asymptomatic Bacterial Vaginosis Among Young African American Women: A Repeated-Measures Latent Class Analysis.

• DOI: 10.1097/olq.0000000000001256
• 发表时间: 2020-12
• 期刊: Sexually transmitted diseases
• 影响因子: 3.1
• 作者: Coudray MS;Sheehan DM;Li T;Cook RL;Schwebke J;Madhivanan P
• 通讯作者: Madhivanan P