X-ray data analysis in the presence of structural variability
存在结构变异时的 X 射线数据分析
基本信息
- 批准号:9552204
- 负责人:
- 金额:$ 33.52万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-09-22 至 2020-08-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAutomationBehaviorBiologicalBiological ProcessCell physiologyCharacteristicsCommunitiesComplexComplicationComputer softwareCrystallizationDataData AnalysesData CollectionData QualityData SetDescriptorDimensionsDiseaseDrug DesignFoundationsHealthIndividualInternetInvestigationLigand BindingLigandsMapsMeasuresMethodologyMethodsMissionModelingMolecularMorphologic artifactsNoiseOnline SystemsOutcomePharmaceutical PreparationsPhaseProceduresPropertyRadiation-Induced ChangeResearch DesignResearch PersonnelResidual stateRoentgen RaysSamplingSignal TransductionSoftware ToolsSourceSpecimenSpeedStructural ModelsStructureSystemTechniquesTemperatureUncertaintyUnited States National Institutes of HealthValidationWeightWorkX ray diffraction analysisX-Ray Crystallographybasecombinatorialcomputerized data processingdata spacedata structuredesigndetectorelectron densityexperienceexperimental studyinnovationinterestmethod developmentnovelnovel strategiespublic health relevancestructural biologytool
项目摘要
DESCRIPTION (provided by applicant): The proposal "X-ray data analysis in the presence of structural variability" aims to advance diffraction data analysis methods so that the variability between crystals and within crystals is optimally modeled during data processing in reciprocal space and during structural analysis in real space. The significance of the proposed work results from the importance of the technique, which generates uniquely-detailed information. X-ray structures are used to understand cellular processes at the atomic level directly, to explain and validate results obtain by other techniques, to generate hypotheses for detailed studies of cellular process, and to guide drug design studies - all of which are highly relevant to the NIH mission. Macromolecular crystals are frequently of limited size and crystal lattice order. Both may result in the need for combining data from multiple crystals for successful structure solution, with the limited order generating diffraction artifacts and correlating with non-isomorphism between different specimens. Non-isomorphism hinders the averaging of data sets from multiple crystals, because for successful averaging, data need to be very similar. The problems with averaging are compounded by incompleteness of the data in a single data set, radiation-induced changes in the crystal under investigation, and lack of statistical measures that would inform experimenters regarding whether or not the data analysis is progressing in the right direction. There are also technical challenges associated with averaging multiple data sets that result from the combinatorial complexity of data analysis when a large number of data sets need to be analyzed. Final difficulty appears when the analysis of the structural results obtained from multi-crystal experiments must separate the desired biological signals, e.g. the presence of a ligand or a specific dynamic behavior of the molecules, from the noise. Our proposal addresses these problems by developing and implementing innovative approaches. In Aim 1, new approaches will be developed and implemented for averaging multiple, potentially incomplete data sets resulting from one or more crystals. Owing to our innovative approach to modeling the components of non-isomorphism, we expect that even quite non-isomorphous data sets can be used together to solve challenging structures. In Aim 2, methods that will analyze the results of averaging data sets from multiple crystals in real space will be developed. The descriptors of averaging will be correlated with the outcomes of the structural analysis, so that the contributors to variability in real space can be quantified and interpreted. Finally, in Am 3, a web-based server will be developed in order to provide these methods to the structural biology community.
描述(由申请人提供):提案“存在结构变异性的X射线数据分析”旨在改进衍射数据分析方法,以便在倒易空间中的数据处理和真实空间中的结构分析期间对晶体之间和晶体内的变异性进行最佳建模。所提出的工作的重要性源于该技术的重要性,该技术生成独特的详细信息。 X 射线结构用于直接了解原子水平的细胞过程,解释和验证其他技术获得的结果,为细胞过程的详细研究生成假设,并指导药物设计研究 - 所有这些都与 NIH 的任务高度相关。大分子晶体通常具有有限的尺寸和晶格顺序。两者都可能导致需要组合来自多个晶体的数据以实现成功的结构求解,有限阶产生衍射伪影并与不同样本之间的非同构相关。非同构阻碍了对多个晶体的数据集进行平均,因为为了成功平均,数据需要非常相似。由于单个数据集中的数据不完整、所研究的晶体中辐射引起的变化以及缺乏可以告知实验者数据分析是否朝着正确方向进展的统计措施,平均问题变得更加复杂。当需要分析大量数据集时,由于数据分析的组合复杂性,还存在与对多个数据集进行平均相关的技术挑战。当多晶实验获得的结构结果的分析必须分离所需的生物信号时,最终的困难出现了。来自噪声的配体的存在或分子的特定动态行为。我们的建议通过开发和实施创新方法来解决这些问题。在目标 1 中,将开发和实施新方法,用于对一个或多个晶体产生的多个可能不完整的数据集进行平均。由于我们对非同构组件进行建模的创新方法,我们期望即使是完全非同构的数据集也可以一起使用来解决具有挑战性的结构。在目标 2 中,将开发分析真实空间中多个晶体的数据集平均结果的方法。平均的描述符将与结构分析的结果相关联,以便可以量化和解释真实空间中变异性的贡献者。最后,在 Am 3 中,将开发一个基于网络的服务器,以便向结构生物学界提供这些方法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('WLADEK MINOR', 18)}}的其他基金
Reproducible, Unbiased Ligand Identification Assisted by Artificial Intelligence and Development of Ligand Reference Libraries
人工智能辅助的可重复、公正的配体鉴定和配体参考文库的开发
- 批准号:
10019572 - 财政年份:2019
- 资助金额:
$ 33.52万 - 项目类别:
Reproducible, Unbiased Ligand Identification Assisted by Artificial Intelligence and Development of Ligand Reference Libraries
人工智能辅助的可重复、公正的配体鉴定和配体参考文库的开发
- 批准号:
10200091 - 财政年份:2019
- 资助金额:
$ 33.52万 - 项目类别:
Reproducible, Unbiased Ligand Identification Assisted by Artificial Intelligence and Development of Ligand Reference Libraries
人工智能辅助的可重复、公正的配体鉴定和配体参考文库的开发
- 批准号:
10432049 - 财政年份:2019
- 资助金额:
$ 33.52万 - 项目类别:
Metal binding sites in macromolecular structures
大分子结构中的金属结合位点
- 批准号:
9233159 - 财政年份:2016
- 资助金额:
$ 33.52万 - 项目类别:
Metal binding sites in macromolecular structures
大分子结构中的金属结合位点
- 批准号:
9008644 - 财政年份:2016
- 资助金额:
$ 33.52万 - 项目类别:
Integrated resource for reproducibility in macromolecular crystallography
大分子晶体学重现性的综合资源
- 批准号:
9280987 - 财政年份:2015
- 资助金额:
$ 33.52万 - 项目类别:
X-ray data analysis in the presence of structural variability
存在结构变异时的 X 射线数据分析
- 批准号:
9147618 - 财政年份:2015
- 资助金额:
$ 33.52万 - 项目类别:
Integrated resource for reproducibility in macromolecular crystallography
大分子晶体学重现性的综合资源
- 批准号:
8875830 - 财政年份:2015
- 资助金额:
$ 33.52万 - 项目类别:
Integrated resource for reproducibility in macromolecular crystallography
大分子晶体学重现性的综合资源
- 批准号:
9069902 - 财政年份:2015
- 资助金额:
$ 33.52万 - 项目类别:
Centers for High-Throughput Structure Determination
高通量结构测定中心
- 批准号:
8152878 - 财政年份:2010
- 资助金额:
$ 33.52万 - 项目类别:
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