Super-resolution confocal microscope
超分辨率共焦显微镜
基本信息
- 批准号:9274885
- 负责人:
- 金额:$ 56.63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-04-19 至 2018-04-18
- 项目状态:已结题
- 来源:
- 关键词:10 year oldAtherosclerosisBiological AssayCellsCollectionDataDetectionDevicesDiseaseFluorescenceFluorescence Recovery After PhotobleachingFluorescence Resonance Energy TransferFundingHypersensitivityImageImmuneImmunofluorescence ImmunologicImmunologyInfectionInstitutesInsulin-Dependent Diabetes MellitusJournalsLasersMicroscopeNIH Grants and ContractsNatureNoisePrincipal InvestigatorProteinsProtocols documentationPublishingResearch InstituteResearch PersonnelResearch Project GrantsResolutionRheumatoid ArthritisScanningScienceScientistSignal TransductionSystemTechnologyTissuesUnited States National Institutes of HealthVisible RadiationWorkZika Virusbasebehavioral studydetectorinstrumentintravital imagingnovelphotoactivationreceptor
项目摘要
ABSTRACT
The La Jolla Institute for Allergy and Immunology (LJI) is a premier immunology research
institute in the US whose 24 principal investigators are supported by ~$40 million in NIH grants and
contracts. At LJI we study the behavior of immune cells in diseases prominent in the US and around the
world, including: type 1 diabetes, atherosclerosis, rheumatoid arthritis, and Zika infection. Nearly all of our
scientists use laser scanning confocal imaging to visualize cellular interactions, signaling, and function and the
results have been published in Nature, Science, and other prominent journals. However, to progress further in
this important work, we need a versatile confocal microscope with super-resolution capabilities.
Currently, LJI lacks a dynamic, capable, and technologically current confocal system and needs one that
will yield the most impactful data for each NIH research project. This need includes a host of fluorescence
based assays such as quantitative receptor translocation, protein co-localization intracellularly and in tissue,
fluorescence resonance energy transfer (FRET), and fluorescence recovery after photobleaching (FRAP), and
photoactivation. We have a 10 year old Leica SP5 combined confocal and multiphoton system that is
dedicated to intravital imaging and a simple automated Olympus FV10i confocal suitable for fixed cells and
tissues at lower resolutions. LJI has no super-resolution capability. Here, we seek support for purchasing a
state-of-the-art Zeiss 880 confocal microscope with an Airyscan super-resolution detector, highly
sensitive detection devices (GaAsP detectors), the greatest signal-to-noise ratio system for both fixed
and live acquisition, multiple laser lines covering the entire visible light spectrum, and a fully tunable
spectral emission collection grating. Airyscan is a novel super-resolution technology that can achieve 140
nm resolution using standard immunofluorescence protocols. After comparing all instruments on the market, it
is clear that the Zeiss LSM 880 system is best suited for the needs of LJI investigators and, if funded, it will
undoubtedly have a significant impact on NIH-supported scientific progress.
摘要
拉霍亚过敏和免疫学研究所(LJI)是一个首屈一指的免疫学研究机构,
美国的一个研究所,其24名主要研究人员得到了大约4000万美元的NIH赠款的支持,
合同.在LJI,我们研究免疫细胞在美国和周边地区突出疾病中的行为。
1型糖尿病、动脉粥样硬化、类风湿性关节炎和寨卡病毒感染。我们几乎所有的
科学家们使用激光扫描共聚焦成像来可视化细胞的相互作用、信号传导和功能,
研究结果已发表在《自然》、《科学》和其他著名期刊上。然而,为了在
这项重要的工作,我们需要一个多功能的共焦显微镜与超分辨率的能力。
目前,LJI缺乏一个动态的,有能力的,技术上最新的共焦系统,需要一个
将为每个NIH研究项目产生最有影响力的数据。这种需要包括大量的荧光
基于测定如定量受体易位,细胞内和组织中的蛋白共定位,
荧光共振能量转移(FRET)和光漂白后荧光恢复(FRAP),以及
光活化我们有一个10岁的徕卡SP5组合共聚焦和多光子系统,
专门用于活体成像和简单的自动化Olympus FV10i共聚焦,适用于固定细胞,
低分辨率的组织。LJI没有超分辨率能力。在这里,我们寻求支持购买
最先进的Zeiss 880共聚焦显微镜,配备Airyscan超分辨率探测器,
灵敏的探测器件(GaAsP探测器),最大的信噪比系统,
和实时采集,多条激光线覆盖整个可见光谱,以及完全可调的
光谱发射收集光栅。Airyscan是一种新颖的超分辨率技术,可以实现140
nm分辨率。在比较了市场上的所有工具后,
很明显,蔡司LSM 880系统最适合LJI调查人员的需求,如果获得资助,它将
无疑对NIH支持的科学进步产生了重大影响。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Structure of the rabies virus glycoprotein trimer bound to a prefusion-specific neutralizing antibody.
- DOI:10.1126/sciadv.abp9151
- 发表时间:2022-06-17
- 期刊:
- 影响因子:13.6
- 作者:
- 通讯作者:
Spatial and functional arrangement of Ebola virus polymerase inside phase-separated viral factories.
- DOI:10.1038/s41467-023-39821-7
- 发表时间:2023-07-13
- 期刊:
- 影响因子:16.6
- 作者:Fang, Jingru;Castillon, Guillaume;Phan, Sebastien;McArdle, Sara;Hariharan, Chitra;Adams, Aiyana;Ellisman, Mark H.;Deniz, Ashok A.;Saphire, Erica Ollmann
- 通讯作者:Saphire, Erica Ollmann
Proximity interactome analysis of Lassa polymerase reveals eRF3a/GSPT1 as a druggable target for host-directed antivirals.
- DOI:10.1073/pnas.2201208119
- 发表时间:2022-07-26
- 期刊:
- 影响因子:11.1
- 作者:
- 通讯作者:
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Klaus F. Ley其他文献
Binding of function‐blocking mAbs to mouse and human P‐selectin glycoprotein ligand‐1 peptides with and without tyrosine sulfation
功能阻断单克隆抗体与小鼠和人 P-选择素糖蛋白配体 1 肽(有或没有酪氨酸硫酸化)的结合
- DOI:
- 发表时间:
2002 - 期刊:
- 影响因子:5.5
- 作者:
Aravinda Thatte;S. Ficarro;K. Snapp;M. Wild;D. Vestweber;D. Hunt;Klaus F. Ley - 通讯作者:
Klaus F. Ley
Serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) correlate with severity of ileitis in experimental Crohn's disease: A novel marker of small intestinal inflammation
- DOI:
10.1016/s0016-5085(00)85325-1 - 发表时间:
2000-04-01 - 期刊:
- 影响因子:
- 作者:
Jesus Rivera-Nieves;R. Cartland Burns;Christopher A. Moskaluk;Theresa T. Pizarro;Klaus F. Ley;Fabio Cominelli - 通讯作者:
Fabio Cominelli
α<sub>4</sub>β<sub>1</sub>integrin (VLA-4) blockade reduces neointimal growth after carotid air desiccation injury in the ApoE (−/−) mouse
- DOI:
10.1016/s0735-1097(02)80085-7 - 发表时间:
2002-03-06 - 期刊:
- 影响因子:
- 作者:
Kurt G. Barringhaus;J.William Phillips;John M. Sanders;Ann C. Czamik;Klaus F. Ley;Ian J. Sarembock - 通讯作者:
Ian J. Sarembock
Klaus F. Ley的其他文献
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{{ truncateString('Klaus F. Ley', 18)}}的其他基金
Mechanism of kindlin-3-dependent integrin activation
kindlin-3依赖性整合素激活机制
- 批准号:
10676897 - 财政年份:2020
- 资助金额:
$ 56.63万 - 项目类别:
Mechanism of kindlin-3-dependent integrin activation
kindlin-3依赖性整合素激活机制
- 批准号:
10229369 - 财政年份:2020
- 资助金额:
$ 56.63万 - 项目类别:
Vascular macrophages and T cells in atherosclerosis
动脉粥样硬化中的血管巨噬细胞和 T 细胞
- 批准号:
10112954 - 财政年份:2019
- 资助金额:
$ 56.63万 - 项目类别:
Vascular macrophages and T cells in atherosclerosis
动脉粥样硬化中的血管巨噬细胞和 T 细胞
- 批准号:
10369710 - 财政年份:2019
- 资助金额:
$ 56.63万 - 项目类别:
Vascular macrophages and T cells in atherosclerosis
动脉粥样硬化中的血管巨噬细胞和 T 细胞
- 批准号:
9895858 - 财政年份:2019
- 资助金额:
$ 56.63万 - 项目类别:
Vascular macrophages and T cells in atherosclerosis
动脉粥样硬化中的血管巨噬细胞和 T 细胞
- 批准号:
10623034 - 财政年份:2019
- 资助金额:
$ 56.63万 - 项目类别:
Vascular macrophages and T cells in atherosclerosis
动脉粥样硬化中的血管巨噬细胞和 T 细胞
- 批准号:
10565907 - 财政年份:2019
- 资助金额:
$ 56.63万 - 项目类别:
Core E: Cell sorting, CyTOF and RNA-Seq
核心 E:细胞分选、CyTOF 和 RNA-Seq
- 批准号:
10188604 - 财政年份:2017
- 资助金额:
$ 56.63万 - 项目类别:
Core B: Single Cell Protein and RNA Sequencing Core
核心 B:单细胞蛋白质和 RNA 测序核心
- 批准号:
10334092 - 财政年份:2017
- 资助金额:
$ 56.63万 - 项目类别:
Project 4: APOB-specific CD4 and CD8 T cells exacerbate atherosclerosis
项目4:APOB特异性CD4和CD8 T细胞加剧动脉粥样硬化
- 批准号:
10334097 - 财政年份:2017
- 资助金额:
$ 56.63万 - 项目类别:
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