Ixodes scapularis and Amblyomma americanum tick cement proteome

肩胛硬蜱和美洲钝蜱蜱水泥蛋白质组

• 批准号: 9321502
• 负责人: ALBERT MULENGA
• 金额: $ 1.28万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9321502
• 关键词: Adhesives; Amblyomma; Anaplasma phagocytophilum; Animals; Arthropod Vectors; Babesia microti; Biopsy; Bite; Black-legged Tick; Blood; Borrelia burgdorferi; Collaborations; Cone; Containment; Data; Deposition; Development; Disease; Dissection; Event; Future; Glues; Goals; Grooming; Health; Human; Ixodidae; Lasers; Lesion; Letters; Lyme Disease; Methods; Physiology; Process; Proteins; Proteome; Protocols documentation; Public Health; RNA Interference; Rickettsia rickettsii; Sequence Analysis; Site; Skin; Tick Control; Tick-Borne Diseases; Ticks; Time; United States; Vector-transmitted infectious disease; acaricide; base; combinatorial; comparative; feeding; human disease; improved; novel; prevent; programs; protein function; success; sucking; transmission process

 DESCRIPTION (provided by applicant): We propose to identify and characterize proteins in innermost layer of Ixodes scapularis and Amblyomma americanum tick cement cones. Currently there are 14 reportable human tick borne disease (TBD) agents are recognized in the United States with 13 of these transmitted via hard tick bites (http://www.cdc.gov/ticks/diseases/). Hard tick species are adapted to feed for many days. Majority of hard ticks secrete an adhesive substance known as "tick cement" that allows ticks to firmly anchor onto host skin, and prevent them from being groomed off their feeding site by their host. The tick cement cone has two layers: the innermost layer, which is deposited within 5-30min of the tick starting the feeding process, and the outermost layer, which begins to be deposited one day after the tick has started the feeding process. Deposition of the innermost layer precedes major tick feeding events including transmission of major human tick borne diseases such as the Lyme disease agent. Thus, our assumptions are that preventing functions of proteins that form the innermost layer of the tick cement cone will stop tick feeding before transmission of major human disease agents starts. There are two specific aims in this application. First is to identify proteins in I. scapularis and A. americanum tick cement cones, and second is to verify significance in tick attachment and/or completion of tick feeding. Of the 14 reportable human TBD agents, in the United States, a combined 9 are vectored by I. scapularis and A. americanum (http://www.cdc.gov/ticks/diseases/). Thus proposed discovery of I. scapularis and A. americanum tick cement proteins will have broader impacts toward development of novel methods to improve public health in the USA.

 描述(由申请人提供)：我们建议鉴定和表征肩部硬蜱和美洲Amblyomma americanum tick水泥锥体最内层的蛋白质。目前，美国已确认有14种可报告的人类壁虱传播疾病病原体，其中13种是通过硬壁虱叮咬(http://www.cdc.gov/ticks/diseases/).)传播的硬蜱物种适应了许多天的觅食。大多数硬壁虱都会分泌一种被称为“壁虱粘合剂”的粘性物质，它能让壁虱牢牢地固定在寄主皮肤上，防止它们被寄主清理出它们的觅食场所。壁虱水泥锥体有两层：最里面的一层，在开始喂食过程后5-30分钟内沉积；最外面的一层，在开始喂食过程一天后开始沉积。最内层的沉积发生在重要的扁虱饲养事件之前，包括传播主要的人类扁虱传播疾病，如莱姆病病原体。因此，我们的假设是，在主要人类疾病病原体开始传播之前，形成扁虱水泥锥体最内层的蛋白质的预防功能将阻止扁虱进食。这个应用程序有两个特定的目标。首先是鉴定I中的蛋白质。 第二个目的是为了验证扁虱附着和/或完成扁虱摄食的重要性。在14个可报告的人类慢性阻塞性肺病病原体中，在美国，总共有9个病原体是由肩周炎和美洲钩端螺旋体(http://www.cdc.gov/ticks/diseases/).)传播的。因此，提出的肩胛骨和美洲棘豆粘骨蛋白的发现将对开发新的方法来改善美国的公共健康产生更广泛的影响。