Ixodes scapularis and Amblyomma americanum tick cement proteome

肩胛硬蜱和美洲钝蜱蜱水泥蛋白质组

• 批准号: 9111224
• 负责人: ALBERT MULENGA
• 金额: $ 22.28万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9111224
• 关键词: Adhesives; Amblyomma; Anaplasma phagocytophilum; Animals; Arthropod Vectors; Babesia microti; Biopsy; Bite; Black-legged Tick; Blood; Borrelia burgdorferi; Collaborations; Cone; Containment; Data; Deposition; Development; Disease; Dissection; Event; Future; Glues; Goals; Grooming; Human; Ixodidae; Lasers; Lesion; Letters; Lyme Disease; Methods; Physiology; Process; Proteins; Proteome; Protocols documentation; Public Health; RNA Interference; Rickettsia rickettsii; Sequence Analysis; Site; Skin; Tick Control; Tick-Borne Diseases; Ticks; Time; United States; Vector-transmitted infectious disease; acaricide; base; combinatorial; comparative; feeding; human disease; improved; novel; prevent; programs; protein function; public health relevance; success; sucking; transmission process

 DESCRIPTION (provided by applicant): We propose to identify and characterize proteins in innermost layer of Ixodes scapularis and Amblyomma americanum tick cement cones. Currently there are 14 reportable human tick borne disease (TBD) agents are recognized in the United States with 13 of these transmitted via hard tick bites (http://www.cdc.gov/ticks/diseases/). Hard tick species are adapted to feed for many days. Majority of hard ticks secrete an adhesive substance known as "tick cement" that allows ticks to firmly anchor onto host skin, and prevent them from being groomed off their feeding site by their host. The tick cement cone has two layers: the innermost layer, which is deposited within 5-30min of the tick starting the feeding process, and the outermost layer, which begins to be deposited one day after the tick has started the feeding process. Deposition of the innermost layer precedes major tick feeding events including transmission of major human tick borne diseases such as the Lyme disease agent. Thus, our assumptions are that preventing functions of proteins that form the innermost layer of the tick cement cone will stop tick feeding before transmission of major human disease agents starts. There are two specific aims in this application. First is to identify proteins in I. scapularis and A. americanum tick cement cones, and second is to verify significance in tick attachment and/or completion of tick feeding. Of the 14 reportable human TBD agents, in the United States, a combined 9 are vectored by I. scapularis and A. americanum (http://www.cdc.gov/ticks/diseases/). Thus proposed discovery of I. scapularis and A. americanum tick cement proteins will have broader impacts toward development of novel methods to improve public health in the USA.

 描述（由申请方提供）：我们建议鉴定和表征肩突硬蜱和美洲钝眼蜱粘固锥最内层的蛋白质。目前，在美国有14种可报告的人蜱传播疾病（TBD）病原体，其中13种通过硬蜱叮咬传播（http：//www.cdc.gov/ticks/diseases/）。硬蜱种类适应于进食许多天。大多数硬蜱分泌一种称为“蜱粘固剂”的粘性物质，使蜱牢固地锚在宿主皮肤上，并防止它们被宿主梳理离开进食部位。蜱粘固剂锥有两层：最内层，在蜱开始进食过程的5- 30分钟内沉积，最外层，在蜱开始进食过程后一天开始沉积。最内层的沉积先于主要的蜱虫进食事件，包括传播主要的人类蜱虫传播疾病，如莱姆病病原体。因此，我们的假设是，阻止形成蜱虫胶结锥最内层的蛋白质的功能将在主要人类疾病病原体开始传播之前阻止蜱虫进食。本申请有两个具体目的。首先是鉴定I. scapularis和A.美洲蜱粘固剂锥，第二是验证蜱附着和/或完成蜱喂养的重要性。在14种可报告的人类TBD病原体中，在美国，合并的9种由I. scapularis和A. americanum（http：//www.cdc.gov/ticks/diseases/）。因此提出了发现I. scapularis和A.美洲蜱粘固蛋白将对开发新方法以改善美国公共卫生产生更广泛的影响。