Organoid Cultivation Core
Organoid Cultivation Core
批准号:
10357967
负责人:
SARAH E BLUTT
金额:
$40.04万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
未结题
起止时间:
2021-03-01 至 2026-02-28
关键词:
AgeAntibiotic ResistanceAntimicrobial ResistanceBacteriaBacterial Drug ResistanceBacterial InfectionsBacteriophagesBiologicalBiopsyBladderCombating Antibiotic Resistant BacteriaCommunicable DiseasesCommunicationCommunitiesCost SavingsDevelopmentDiseaseElementsEnsureEpithelialEquipment and SuppliesEthnic OriginFreezingFunctional disorderFundingGastrointestinal tract structureGenomic Centers for Infectious DiseasesGoalsGrowthGrowth FactorHealthHumanHuman ResourcesIndividualInfectionIntegration Host FactorsInterdisciplinary StudyIntestinesLeadershipLiverMaintenanceMeasuresModelingMucous MembraneNasal EpitheliumNoseOperative Surgical ProceduresOrganoidsOutcomePathogenesisPatientsPhysiologicalPhysiologyPlayPre-Clinical ModelProductionQuality ControlRadiation induced damageReproducibilityResearchResearch PersonnelResearch Project GrantsResistance to infectionRoleSamplingSpecimenStandardizationSurfaceSystemTechnical ExpertiseTechnology TransferTestingTherapeuticTimeTranslatingUnited States National Institutes of HealthVaginaViralWorkairway epitheliumcombatcostcost effectivedemographicsepithelial repairgastrointestinalhost microbiomeinterestintestinal epitheliummeetingsmembermicrobialmicrobiomemicrobiome compositionmicroorganismnovelpathogenresponsesexstem cellssuccesstraining project
中文摘要
项目总结
U19抗击抗生素耐药细菌(CARB)跨学科研究小组(CARBIRU)的目标
应用是为了更深入地了解细菌和宿主因素在抗菌中的作用
抵抗力和感染。为了实现这一目标,有机化合物培养核心(OCC)的建立将使
提供关键的与生物相关的人类器官型培养,这是成功的关键
项目。OCC将围绕两个目标进行组织:1)开发从
供个人项目使用的阴道、膀胱和鼻腔;(2)生产用于
每个项目都要协助实现各自规定的研究目标。这些目标将提供测试的能力
关于宿主上皮作用的关键假设和开发的关键结果可以转化为
了解细菌感染和抗菌素耐药性。核心将减少劳动力、供应和设备
通过集中隔离、增长和维护
有机体线条。大量获取培养有机物质所需的材料,条件培养基标准化
包含增长因素,以及线路维护的集中化导致大量时间和成本
为个别项目调查人员节省成本,并确保标准化和重现性。此外,
控制中心核心将加强项目之间的沟通和成果的可比性。OCC核心
将是本应用程序中每个项目成功的重要组成部分,也是一个统一的
将它们连接在一起的元素。
英文摘要
PROJECT SUMMARY
The goal of this U19 Combating Antibiotic Resistant Bacteria (CARB) Interdisciplinary Research Unit (CARBIRU)
application is to gain a deeper understanding of bacteria and host factors that play a role in antibacterial
resistance and infection. To meet this goal, the establishment of the Organoid Cultivation Core (OCC) will allow
the provision of critical biologically relevant human organotypic cultures that are essential for the success of the
project. The OCC will be organized around two goals: 1) development of human derived organoids from the
vagina, the bladder and the nasal airway for use by the individual projects and (2) production of organoids for
each project to assist in meeting their individual stated research goals. These goals will offer the ability to test
key hypotheses about the role of the host epithelium and develop key outcomes that can be translated into
understanding bacterial infections and antibacterial resistance. The core will reduce labor, supply and equipment
costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the
organoid lines. Bulk acquisition of materials required to culture organoids, standardization of conditioned media
containing growth factors, and centralization of the maintenance of the lines result in significant time and cost
savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the
OCC core will enhance communication and comparability of results obtained between projects. The OCC core
will be an essential component of the success of each of the projects in this application as well as a unifying
element in connecting them together.
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会议论文
Organoid Cultivation Core
-
批准号:10583461
-
项目类别:
-
资助金额:$25.96万
-
财政年份:2021
-
负责人:SARAH E BLUTT
-
依托单位:
Organoid and Minibioreactor Array Cultivation Core
-
批准号:10601136
-
项目类别:
-
资助金额:$55.71万
-
财政年份:2019
-
负责人:SARAH E BLUTT
-
依托单位:
Organoid and Minibioreactor Array Cultivation Core
-
批准号:10160784
-
项目类别:
-
资助金额:$16.98万
-
财政年份:2019
-
负责人:SARAH E BLUTT
-
依托单位:
Organoid and Minibioreactor Array Cultivation Core
-
批准号:10396595
-
项目类别:
-
资助金额:$55.71万
-
财政年份:2019
-
负责人:SARAH E BLUTT
-
依托单位:
Human Biomimetic Scientific Core
-
批准号:10642940
-
项目类别:
-
资助金额:$33.83万
-
财政年份:2015
-
负责人:SARAH E BLUTT
-
依托单位:
Human Biomimetic Scientific Core
-
批准号:10192206
-
项目类别:
-
资助金额:$23.53万
-
财政年份:2015
-
负责人:SARAH E BLUTT
-
依托单位:
Human Biomimetic Scientific Core
-
批准号:10462789
-
项目类别:
-
资助金额:$24.29万
-
财政年份:2015
-
负责人:SARAH E BLUTT
-
依托单位:
Immune Mechansims of Rotavirus Protection & Clearanace
-
批准号:6423066
-
项目类别:
-
资助金额:$4.62万
-
财政年份:2002
-
负责人:SARAH E BLUTT
-
依托单位:
Gastrointestinal Experimental Model Systems Core
-
批准号:10576214
-
项目类别:
-
资助金额:$17.02万
-
财政年份:2001
-
负责人:SARAH E BLUTT
-
依托单位:
Immune Mechansims of Rotavirus Protection & Clearance
-
批准号:6340149
-
项目类别:
-
资助金额:$4.02万
-
财政年份:2001
-
负责人:SARAH E BLUTT
-
依托单位:
Organoid and Minibioreactor Array Cultivation Core
-
批准号:9915900
-
项目类别:
-
资助金额:$17.2万
-
财政年份:--
-
负责人:SARAH E BLUTT
-
依托单位:
海外基金