Organoid Cultivation Core

项目摘要 这个U19对抗抗生素耐药细菌（CARB）跨学科研究单位（CARBIRU）的目标 应用是为了更深入地了解细菌和宿主因素，在抗菌作用中发挥作用， 抵抗和感染。为了实现这一目标，类器官培养核心（OCC）的建立将允许 提供关键的生物学相关的人类器官型培养物，这对成功的 项目OCC将围绕两个目标进行组织：1）从人源性类器官的开发 阴道、膀胱和鼻气道供个人项目使用，以及（2）产生类器官， 每个项目，以协助满足其各自的研究目标。这些目标将提供测试能力， 关于宿主上皮作用的关键假设，并开发可转化为 了解细菌感染和抗菌素耐药性。核心将减少劳动力、供应和设备 成本和完成项目所需的时间， 类器官线培养类器官所需材料的批量获取，条件培养基的标准化 含有生长因子，以及生产线维护的集中化导致了显著的时间和成本 为项目调查人员节省费用，并确保标准化和可重复性。另夕h OCC核心将加强项目之间的沟通和成果的可比性。OCC核心 将是每个项目在这个应用程序中的成功，以及统一的重要组成部分， 元素将它们连接在一起。

PROJECT SUMMARY The goal of this U19 Combating Antibiotic Resistant Bacteria (CARB) Interdisciplinary Research Unit (CARBIRU) application is to gain a deeper understanding of bacteria and host factors that play a role in antibacterial resistance and infection. To meet this goal, the establishment of the Organoid Cultivation Core (OCC) will allow the provision of critical biologically relevant human organotypic cultures that are essential for the success of the project. The OCC will be organized around two goals: 1) development of human derived organoids from the vagina, the bladder and the nasal airway for use by the individual projects and (2) production of organoids for each project to assist in meeting their individual stated research goals. These goals will offer the ability to test key hypotheses about the role of the host epithelium and develop key outcomes that can be translated into understanding bacterial infections and antibacterial resistance. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines. Bulk acquisition of materials required to culture organoids, standardization of conditioned media containing growth factors, and centralization of the maintenance of the lines result in significant time and cost savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the OCC core will enhance communication and comparability of results obtained between projects. The OCC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.