Organoid Cultivation Core

项目总结 U19抗击抗生素耐药细菌(CARB)跨学科研究小组(CARBIRU)的目标 应用是为了更深入地了解细菌和宿主因素在抗菌中的作用 抵抗力和感染。为了实现这一目标，有机化合物培养核心(OCC)的建立将使 提供关键的与生物相关的人类器官型培养，这是成功的关键 项目。OCC将围绕两个目标进行组织：1)开发从 供个人项目使用的阴道、膀胱和鼻腔；(2)生产用于 每个项目都要协助实现各自规定的研究目标。这些目标将提供测试的能力 关于宿主上皮作用的关键假设和开发的关键结果可以转化为 了解细菌感染和抗菌素耐药性。核心将减少劳动力、供应和设备 通过集中隔离、增长和维护 有机体线条。大量获取培养有机物质所需的材料，条件培养基标准化 包含增长因素，以及线路维护的集中化导致大量时间和成本 为个别项目调查人员节省成本，并确保标准化和重现性。此外， 控制中心核心将加强项目之间的沟通和成果的可比性。OCC核心 将是本应用程序中每个项目成功的重要组成部分，也是一个统一的 将它们连接在一起的元素。

PROJECT SUMMARY The goal of this U19 Combating Antibiotic Resistant Bacteria (CARB) Interdisciplinary Research Unit (CARBIRU) application is to gain a deeper understanding of bacteria and host factors that play a role in antibacterial resistance and infection. To meet this goal, the establishment of the Organoid Cultivation Core (OCC) will allow the provision of critical biologically relevant human organotypic cultures that are essential for the success of the project. The OCC will be organized around two goals: 1) development of human derived organoids from the vagina, the bladder and the nasal airway for use by the individual projects and (2) production of organoids for each project to assist in meeting their individual stated research goals. These goals will offer the ability to test key hypotheses about the role of the host epithelium and develop key outcomes that can be translated into understanding bacterial infections and antibacterial resistance. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines. Bulk acquisition of materials required to culture organoids, standardization of conditioned media containing growth factors, and centralization of the maintenance of the lines result in significant time and cost savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the OCC core will enhance communication and comparability of results obtained between projects. The OCC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.