Gastrointestinal Experimental Model Systems Core

胃肠实验模型系统核心

• 批准号: 10576214
• 负责人: SARAH E BLUTT
• 金额: $ 17.02万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10576214
• 关键词: Animal Model; Animals; Biological Models; Biological Monitoring; Biomedical Research; Clinical Research; Clinical Trials; Complex; Conditioned Culture Media; Development; Digestive System Disorders; Disease; Doctor of Philosophy; Embryo Transfer; Ensure; Environment; Epithelium; Experimental Models; Freezing; Funding; Gastrointestinal tract structure; Germ-Free; Gnotobiotic; Goals; Growth; Health; Heterogeneity; Homeostasis; Housing; Human; Human Resources; Individual; Infection; Injury; Instruction; Intestines; Leadership; Medical center; Metabolism; Microbe; Mission; Modeling; Mus; Operative Surgical Procedures; Organ; Organism; Organoids; Patients; Physiology; Pre-Clinical Model; Protocols documentation; Publications; Quality Control; Reagent; Research; Research Design; Rodent; Services; Signal Transduction; Specific qualifier value; Specimen; Standardization; Stromal Cells; System; Technology; Technology Transfer; Texas; Time; Tissues; Training; Validation; cell type; cost; experience; experimental study; functional genomics; gastrointestinal; human pluripotent stem cell; improved; in vivo; interest; member; microbial; microbial community; microbial composition; microbiome; molecular imaging; mouse model; organizational structure; outreach; stem cell derived tissues; stem cells; training project

PROJECT SUMMARY: Gastrointestinal Experimental Model Systems Core (Biomedical Research Core) The Gastrointestinal Experimental Model Systems (GEMS) Core meets the experimental and scientific needs of the members of the Texas Medical Center (TMC) Digestive Disease Center (DDC), and supports the DDC’s overall theme and mission. The GEMS Core is subdivided into two subcore: 1) organoid, and 2) gnotobiotic sub-cores. The Core’s centralized services support exciting scientific developments, are based on the unique expertise of the Core leadership, and facilitate the use of cutting-edge model systems by DDC members. The Core maintains and supplies DDC members with experiment-ready organoid cultures and gnotobiotic rodents. The Core’s organizational structure reduces cost burden for members, increases efficiency, saves time, and provides quality control for each project. The overall goal of the GEMS Core is to provide DDC members with state-of-the-art experimental model systems that are difficult to initiate and maintain in independent labs. There is no similar facility in the TMC or the region. The Specific Aims of the GEMS Core are to: 1) generate, maintain, and provide experiment-ready gastrointestinal tract organoid cultures and specialized growth reagents for membership use; 2) increase the complexity of organoid models to study infection and injury; 3) produce, house, manipulate, and colonize gnotobiotic animals per the needs of DDC members; and, 4) establish housing and embryo transfer conditions to improve the overall efficiency of the gnotobiotic model. The GEMS Core has extensive interactions with the Study Design and Clinical Research Core, the Tissue Analysis and Molecular Imaging Core, and the Functional Genomics and Microbiome Core. The GEMS Core will continue to respond to the needs of DDC members and develop new services to meet them. The Core has been heavily used by the 103 DDC members this cycle: organoid subcore by at least 31, gnotobiotics by 29 with nearly 3000 service requests resulting in over 80 publications.

项目摘要：胃肠道实验模型系统核心(生物医学研究 核心) 胃肠道实验模型系统(GEMS)核心可满足 德克萨斯医疗中心(TMC)消化疾病中心(DDC)的成员，并支持DDC的 总的主题和使命。 宝石岩芯又细分为两个亚核：1)有机亚核和2)生物亚核。核心的 集中服务支持令人兴奋的科学发展，基于Core的独特专业知识 领导力，并促进DDC成员使用尖端模型系统。核心维护和 为DDC成员提供可供实验使用的有机培养物和灵生啮齿类动物。核心的 组织结构减轻了成员的成本负担，提高了效率，节省了时间，提供了质量 控件为每个项目创建。GEMS核心的总体目标是为DDC成员提供最先进的 难以在独立实验室中启动和维护的实验模型系统。没有类似的 在TMC或该地区的设施。 GEMS核心的具体目标是：1)生成、维护和提供实验准备 会员使用的胃肠道有机培养物和专用生长试剂；2)增加 研究感染和损伤的有机体模型的复杂性；3)生产、安置、操作和定居 符合DDC成员需要的灵知生物动物；以及，4)建立住房和胚胎移植条件 提高灵知生菌模型的整体效率。 GEMS核心与研究设计和临床研究核心、组织 分析和分子成像核心，以及功能基因组和微生物组核心。宝石核心将 继续回应DDC成员的需求，并开发新的服务来满足他们。核心一直是 这一周期被103个DDC成员大量使用：有机亚核至少增加31个，灵知生物增加29个，几乎 3000项服务请求，产生了80多份出版物。