Human Biomimetic Scientific Core

项目摘要-核心B 这个U19仿生学合作研究中心(BCRC)应用程序的目标之一是使用 -ART平台，解决有关病毒和细菌生物体致病机理的知识空白，请访问 粘膜表面。为了实现这一目标，建立人类仿生科学核心将 允许提供关键的与生物相关的仿生人体有机平台，这些平台对于 项目的成功。核心将围绕两个目标进行组织：(1)生成、维护和提供 经实验准备的肠道和呼吸道有机培养物和专用生长试剂 本申请中提出的项目，以及(2)通过整合 神经元、免疫和微生物组分，以改进有机类化合物作为3D模拟模型。这些目标 将促进呼吸道和肠道传染病的研究。核心将减少劳动力、供应和 通过集中隔离、增长和维护来完成项目所需的设备成本和时间 并投入时间和资源来增加培养的复杂性。批量收购 材料、介质和其他试剂的标准化，以及线路维护的集中化 为各个项目调查人员节省大量时间和成本，并确保标准化 和再现性。HBSC将为每个项目人员提供培训和建议，并制定 与主要调查人员密切互动关系，允许优化和定制核心 在项目中使用的试剂。《儿童权利公约》的核心将是每个国家成功的重要组成部分 此应用程序中的项目以及将它们连接在一起的统一元素。

PROJECT SUMMARY – Core B One of the goals of this U19 Biomimetics Cooperative Research Center (BCRC) application is to use state-of- the-art platforms to address gaps in knowledge surrounding pathogenesis of viral and bacterial organisms at mucosal surfaces. To meet this goal, the establishment of the Human Biomimetic Scientific Core (HBSC) will allow the provision of critical biologically relevant human biomimetic organoid platforms that are essential for the success of the project. The core will be organized around two goals: (1) generate, maintain, and provide experimentally ready intestinal and airway organoid cultures and specialized growth reagents for use in the projects proposed in this application, and (2) increase the complexity of organoid cultures by the integration of neuronal, immune, and microbiome components to improve the organoids as 3D mimetic models. These goals will facilitate infectious disease research in the airway and intestine. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines and devote time and resources to increasing the complexity of the cultures. Bulk acquisition of materials, standardization of media and other reagents, and centralization of the maintenance of the lines results in significant time and cost savings to the individual project investigators and ensures standardization and reproducibility. The HBSC will provide training and advice to each of the project personnel and develop a close interactive relationship with the principle investigators that will allow optimization and customization of core reagents for use in the projects. The BCRC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.