Human Biomimetic Scientific Core

项目概要-核心B U19仿生学合作研究中心（BCRC）申请的目标之一是使用 最先进的平台，以解决围绕病毒和细菌生物体发病机制的知识差距， 粘膜表面。为了实现这一目标，人类仿生科学核心（HBSC）的建立将 允许提供关键的生物学相关的人类仿生类器官平台，这些平台对于 项目的成功。核心将围绕两个目标进行组织：（1）生成、维护和提供 实验准备好的肠道和气道类器官培养物和专门的生长试剂， 本申请中提出的项目，以及（2）通过整合 神经元，免疫和微生物组成分，以改善类器官作为3D模拟模型。这些目标 将促进呼吸道和肠道的传染病研究。核心将减少劳动力，供应和 设备成本，以及通过集中隔离、增长和维护来完成项目所需的时间 并投入时间和资源来增加培养物的复杂性。批量采集 材料的标准化、培养基和其他试剂的标准化以及生产线维护的集中化 为各个项目调查员节省大量时间和费用，并确保标准化 和再现性。HBSC将为每个项目人员提供培训和建议，并制定 与主要研究人员建立密切的互动关系，从而可以优化和定制核心 用于项目的试剂。巴塞尔公约区域中心的核心将是每一个区域中心取得成功的重要组成部分。 项目在这个应用程序中，以及一个统一的元素，将它们连接在一起。

PROJECT SUMMARY – Core B One of the goals of this U19 Biomimetics Cooperative Research Center (BCRC) application is to use state-of- the-art platforms to address gaps in knowledge surrounding pathogenesis of viral and bacterial organisms at mucosal surfaces. To meet this goal, the establishment of the Human Biomimetic Scientific Core (HBSC) will allow the provision of critical biologically relevant human biomimetic organoid platforms that are essential for the success of the project. The core will be organized around two goals: (1) generate, maintain, and provide experimentally ready intestinal and airway organoid cultures and specialized growth reagents for use in the projects proposed in this application, and (2) increase the complexity of organoid cultures by the integration of neuronal, immune, and microbiome components to improve the organoids as 3D mimetic models. These goals will facilitate infectious disease research in the airway and intestine. The core will reduce labor, supply and equipment costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the organoid lines and devote time and resources to increasing the complexity of the cultures. Bulk acquisition of materials, standardization of media and other reagents, and centralization of the maintenance of the lines results in significant time and cost savings to the individual project investigators and ensures standardization and reproducibility. The HBSC will provide training and advice to each of the project personnel and develop a close interactive relationship with the principle investigators that will allow optimization and customization of core reagents for use in the projects. The BCRC core will be an essential component of the success of each of the projects in this application as well as a unifying element in connecting them together.