Histology, Biomechanics, and Human Tissue Core

组织学、生物力学和人体组织核心

• 批准号: 10495744
• 负责人: Jinhu Xiong
• 金额: $ 22.06万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-16 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10495744
• 关键词: Animals; Arkansas; Bioinformatics; Biology; Biomechanics; Bone Marrow; Bone Marrow Cells; Bone Tissue; Clinical; Clinical Data; Collaborations; DNA; Decalcification; Disease; Doctor of Philosophy; Educational Activities; Educational workshop; Endocrinology; Ensure; Equipment; Faculty; Frozen Sections; Gene Expression; Genetic; Goals; Hindlimb Suspension; Hip region structure; Histologic; Histology; Human; Human Resources; Image; In Situ; Institution; Knowledge; Laboratories; Maintenance; Measurement; Medical; Mesenchymal Stem Cells; Metastatic Neoplasm to the Bone; Microtomy; Molecular; Mus; Musculoskeletal; Musculoskeletal Diseases; Operative Surgical Procedures; Orthopedics; Osteogenesis; Osteoporosis; Paraffin Embedding; Pathologic; Patient Care; Patients; Phase; Physiological; Plasma; Plastic Embedding; Process; RNA; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resources; Sampling; Science; Serum; Services; Skeleton; Stains; System; Testing; Thick; Tissue Sample; Tissues; Training; Training and Education; Translational Research; Universities; Urine; Work; biobank; biomechanical test; bone; bone imaging; design; experience; experimental study; hip replacement arthroplasty; human tissue; in vivo; investigator training; long bone; member; mouse model; new technology; novel; programs; research faculty; skeletal; skeletal disorder; spine bone structure; success; tibia; transcriptomics

PROJECT SUMMARY/ABSTRACT – CORE C The goal of the Histology, Biomechanics, and Human Tissue Core (Core C) is to provide state-of-the-art services for the characterization of tissue and cellular changes that underlie the pathological mechanisms of skeletal diseases in both mice and humans. The core will provide 3 services: 1) high quality and reliable hard- tissue histology services that will help investigators identify the cellular basis of musculoskeletal changes and formulate new mechanistic hypotheses for disease processes; 2) comprehensive in vivo and ex vivo biomechanical testing to quantify the mechano-responsiveness, quality, and strength of the skeleton under physiological and pathological conditions; and 3) banking and access to human tissue samples from hip replacement surgeries and bone marrow cells with their associated clinical data from patients with various conditions. This is a novel component that will expand the translational research opportunities in the Center for Musculoskeletal Disease Research (CMDR) for Project Leaders, CMDR faculty, and eventually additional researchers at the University of Arkansas for Medical Sciences (UAMS) and affiliated institutions. The core will be essential to accomplish the goals of each Phase 2 research project. Core C will extensively interact with the other cores of the CMDR by providing histological and biomechanical analysis for new mouse models created by the Genetic Mouse Model Core (Core B), working with the Bone Imaging Core (Core D) for biomechanical analysis and providing samples for histomorphometric analysis, and collaborating with the Bioinformatics Core (Core E) for developing spatial transcriptomics. Overall, the Histology, Biomechanics, and Human Tissue Core provides unique and essential services and thereby significantly expands the musculoskeletal research infrastructure at UAMS and its affiliates.

项目总结/摘要-核心C 组织学、生物力学和人体组织核心（核心C）的目标是提供最先进的 为表征组织和细胞变化提供服务，这些变化是疾病病理机制的基础。 小鼠和人类的骨骼疾病。核心将提供3项服务：1）高品质可靠的硬- 组织组织学服务，这将有助于研究人员确定肌肉骨骼变化的细胞基础， 为疾病过程提出新的机制假说; 2）全面的体内和体外 生物力学测试，以量化机械反应性，质量和骨骼强度， 生理和病理条件;和3）从髋关节中储存和获取人体组织样品 移植手术和骨髓细胞及其相关的临床数据， 条件这是一个新的组成部分，将扩大该中心的翻译研究机会， 肌肉骨骼疾病研究（CMDR）的项目负责人，CMDR教师，并最终额外 研究人员在阿肯色州大学医学科学（UAMS）和附属机构。芯会 是完成每个第二阶段研究项目的目标必不可少的。核心C将广泛地与 通过为创建的新小鼠模型提供组织学和生物力学分析，实现CMDR的其他核心 通过遗传小鼠模型核心（核心B），与骨成像核心（核心D）一起进行生物力学研究 分析和提供样品进行组织形态学分析，并与生物信息学核心合作 (Core（3）发展空间转录组学。总体而言，组织学、生物力学和人体组织核心 提供独特和必要的服务，从而大大扩展了肌肉骨骼研究 UAMS及其附属公司的基础设施。