Cell-type specific characterization of neuronal activity throughout V1

V1 期间神经元活动的细胞类型特异性特征

• 批准号: 10438695
• 负责人: NA Ji
• 金额: $ 38.75万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438695
• 关键词: Automobile Driving; Axon; Behavioral; Brain; Calcium; Cells; Complex; Data; Data Analyses; Data Science Core; Data Scientist; Data Set; Data Storage and Retrieval; Development; Electrophysiology (science); Explosion; Feedback; Fluorescence Microscopy; Functional Imaging; Gene Expression Profiling; Head; Image; Imaging technology; In Situ; In Vitro; Investigation; Location; Measurement; Measures; Modeling; Mus; Neurons; Optics; Pattern; Physiological; Population; Protocols documentation; RNA; Resolution; Scanning; Sensory; Specificity; Speed; Stimulus; Synapses; Techniques; Technology; Testing; Theoretical model; Time; V1 neuron; Validation; Work; adaptive optics; awake; base; calcium indicator; cell type; data analysis pipeline; design; experimental study; imaging study; in vivo; in vivo imaging; large scale data; model development; movie; neural circuit; neuronal cell body; predictive modeling; relating to nervous system; response; theories; two-photon; visual stimulus

SUMMARY To constrain and test models of V1, we need to comprehensively characterize the physiological responses of neurons as well as their main inputs in all six cortical layers. However, imaging neuronal cell bodies or axons deep in the cortex has remained a major technical challenge. The Ji lab made critical breakthroughs in applying adaptive optics to correct brain-induced optical aberrations for in vivo two-photon fluorescence microscopy, so that the activity of axons, boutons, and neurons can be captured across the full depth of cortex. Using Bessel focus scanning technology, the Ji lab also demonstrated high speed (30 Hz) volumetric calcium imaging with synaptic resolution in vivo. In awake mice and under different brain states, these technologies will be used to measure neural activity of both cortical neurons and their main sensory and modulatory inputs in response to a concerted set of visual stimuli including natural movies to rigorously test model predictions. Combined with subsequent in vitro functional connectivity mapping and post hoc cell type identification, we will generate, for the first time, datasets where every neuron within V1 is described by its location, responses to known stimuli, connectivity pattern, and cell type, to guide and validate the modeling projects.

概括 为了约束和测试V1模型，我们需要全面表征V1的生理反应 神经元及其在所有六个皮质层中的主要输入。然而，对神经元细胞体或轴突进行成像 皮层深处仍然是一个重大的技术挑战。季教授实验室在以下方面取得重大突破 应用自适应光学来校正体内双光子荧光的脑诱发光学像差 显微镜，以便可以捕获整个皮层深度的轴突、纽扣和神经元的活动。 利用贝塞尔聚焦扫描技术，吉实验室还展示了高速（30 Hz）容量钙 体内突触分辨率成像。在清醒的小鼠和不同的大脑状态下，这些技术将 用于测量皮质神经元的神经活动及其主要感觉和调节输入 对包括自然电影在内的一系列协调一致的视觉刺激做出反应，以严格测试模型预测。 结合后续的体外功能连接图谱和事后细胞类型识别，我们将 第一次生成数据集，其中 V1 中的每个神经元都通过其位置、响应来描述 已知的刺激、连接模式和细胞类型，以指导和验证建模项目。