Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome

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ABSTRACT An estimated 17% of women in Kentucky smoke during pregnancy. The studies proposed within this renewal application use a well-developed murine model of developmental exposure to tobacco smoke. We have shown effects of prenatal cigarette smoke exposure (CSE) on birth weight, catch-up growth, and gut bacterial microbiome in mouse dams and their offspring, as well as effects on offspring phenotype (preliminary data). However, we do not know if the observed changed in microbiome and offspring phenotype are linked. We will measure the effect of cecal transfer of microbiomes or dietary resistant starch on those same measures in this model. Our central hypothesis, that CSE-induced gut microbiome alterations are linked to offspring phenotype, will be tested within 2 aims: Aim 1: use cecal transfer in weanlings to separate effects of CSE-induced microbiome from CSE itself Aim 2: use dietary resistant starch to increase short-chain fatty acids before vs after puberty If our central hypothesis is supported, future studies will delve into gut-liver-brain axis consequences of intestinal dysbiosis, as well as investigate potential interventions. Drs. Corbitt (PI), Neal (co-I), and Kosiewicz (co-I) jointly will train students and conduct the proposed studies.