Automated CRISPR Enabled DNA Synthesis (CEDS)

自动化 CRISPR DNA 合成 (CEDS)

• 批准号: 10369127
• 负责人: Michael David Lynch
• 金额: $ 29.5万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-08 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10369127
• 关键词: AT Rich Sequence; Algorithms; Area; Automation; Base Pairing; Biochemical; CRISPR/Cas technology; Clustered Regularly Interspaced Short Palindromic Repeats; Complex; Cost Savings; Coupling; DNA; DNA Sequence; DNA biosynthesis; DNA sequencing; Decentralization; Development; Directed Molecular Evolution; Engineering; Enzymes; Failure; Gene Pool; Generations; Genes; Goals; Growth; Guide RNA; Individual; Libraries; Ligation; Location; Mediating; Methodology; Methods; Nature; Nucleotides; Oligonucleotides; Plasmids; Price; Provider; RNA Ligase (ATP); Reaction; Repetitive Sequence; Reporting; Single-Stranded DNA; Structure; Technology; Work; Writing; biological research; cost; ds-DNA; gene synthesis; high throughput screening; improved; innovation; mutant; next generation; next generation sequencing; novel; programs; thermostability; tool

Abstract We propose to develop a novel method for low cost DNA synthesis: CEDS (Crispr enabled DNA synthesis). The CEDS approach overcomes many of these challenges and enables template independent, exponential single- stranded DNA synthesis. We have preliminary results supporting key biochemical steps in the CEDS workflow, including both ssDNA ligation and Cpf1 mediated ssDNA cleavage at the target strand. Our longer term goal is to develop the technology to support the overnight synthesis of a 10 kbp plasmid at a cost of <$10.00 which would be $0.001/bp in synthesis cost (not including the costs of sequence verification).

摘要