Automated CRISPR Enabled DNA Synthesis (CEDS)

摘要 我们建议开发一种新的低成本DNA合成方法：CEDS(Crispr Enabled DNA 综合)。CEDS方法克服了其中的许多挑战，并支持模板 独立、指数的单链DNA合成。我们已经有了初步结果 支持CEDS工作流程中的关键生化步骤，包括单链DNA连接和Cpf1 介导的单链DNA在靶链上的切割。我们的长期目标是开发这项技术 为了支持以10.00美元的成本连夜合成10kbp的质粒，这将是 0.001美元/个碱基的合成成本(不包括序列验证的成本)。

Abstract We propose to develop a novel method for low cost DNA synthesis: CEDS (Crispr enabled DNA synthesis). The CEDS approach overcomes many of these challenges and enables template independent, exponential single- stranded DNA synthesis. We have preliminary results supporting key biochemical steps in the CEDS workflow, including both ssDNA ligation and Cpf1 mediated ssDNA cleavage at the target strand. Our longer term goal is to develop the technology to support the overnight synthesis of a 10 kbp plasmid at a cost of <$10.00 which would be $0.001/bp in synthesis cost (not including the costs of sequence verification).