Automated CRISPR Enabled DNA Synthesis (CEDS)

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Abstract We propose to develop a novel method for low cost DNA synthesis: CEDS (Crispr enabled DNA synthesis). The CEDS approach overcomes many of these challenges and enables template independent, exponential single- stranded DNA synthesis. We have preliminary results supporting key biochemical steps in the CEDS workflow, including both ssDNA ligation and Cpf1 mediated ssDNA cleavage at the target strand. Our longer term goal is to develop the technology to support the overnight synthesis of a 10 kbp plasmid at a cost of <$10.00 which would be $0.001/bp in synthesis cost (not including the costs of sequence verification).