Interrogating novel biosynthetic sources for the production of polybrominated diphenyl ethers
探究生产多溴二苯醚的新型生物合成来源
基本信息
- 批准号:10471212
- 负责人:
- 金额:$ 6.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-09-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:AirAlgaeAnabolismAnimalsBacteriaBacterial GenesBiochemicalBivalviaBromineCaribbean regionCatalysisChemicalsCollaborationsConsumptionCouplingCyanobacteriumCytochrome P450DataDietDolphinsEcosystemElectronicsEndocrine disruptionEnvironmentEnvironmental HealthEnzymatic BiochemistryEnzymesFishesFlame RetardantsFlavinsFood ChainFood WebsFurnitureGammaproteobacteriaGenerationsGenesGenomicsGoalsGovernmentHealthHealth HazardsHomologous GeneHormone imbalanceHumanInfant CareInstitutionKineticsLettersLifeLogicMammalsMetabolic BiotransformationMetabolismMetagenomicsMicrobeMolecularNatural ProductsOceanographyOceansOrganismOxidation-ReductionPathway interactionsPoisonPoriferaProductionPseudoalteromonasRed AlgaeResearchRiskSalmonSamplingScombridaeSeafoodSeawaterSite-Directed MutagenesisSoilSourceSystemThyroid HormonesTimeToxic effectTunaWaterWhalesanthropogenesisbioaccumulationdevelopmental diseasedietarydifferential expressionenvironmental chemistryenzyme biosynthesisfeedingfunctional grouphalogenationinsightinterestmanmetabolomicsmetatranscriptomicsmicrobial communitymicrobiotaneurotoxicitynovelocean ecosystemspersistent organic pollutantspolybrominated diphenyl ethersealsymbionttranscriptomics
项目摘要
Project Summary/Abstract
The presence of polybrominated diphenyl ethers (PBDEs) in the ocean has been a human health hazard
since their implementation as flame retardants in the 1970s, causing a myriad of toxic effects including thyroid
hormone imbalances, neurotoxicity, and developmental disorders. Since 2004, the entrance of anthropogenic
PBDEs into the environment has been limited by government restrictions on their production. However, this
environmental health crisis remains as PBDEs continue to be detected around the globe, impacting commonly
consumed seafood, marine mammals, and humans. In 2005, it was discovered that not all PBDEs are of
anthropogenic origin; commonly detected hydroxylated and methoxylated PBDEs (OH-BDEs and MeO-BDEs,
respectively) were found to be natural products. Furthermore, the toxicity of OH-BDEs and MeO-BDEs were
found to surpass that of anthropogenic PBDEs. Studies have demonstrated the persistence of PBDEs and their
hydroxylated and methoxylated congeners in the environment and their ability to be passed through the food
web; however, the handful of known OH/MeO-BDE bacterial and algal producers do not account for the global
representation of the molecules in the oceans. Here, I propose to explore the possibility of novel OH/MeO-BDE
producers by examining probable common dietary sources by searching for clues in sea water, ocean sediments,
and marine animal-associated microbiota.
This proposal aims to mine metagenomic and metatranscriptomic data from environmental samples to
assess the distribution of OH/MeO-BDE producers in the environment and the chemistry employed in the
biosynthetic machinery. The sequences of known OH/MeO-BDE biosynthesis enzymes from marine bacteria will
be used initially as probes, followed by the use of biosynthetic logic to identify new enzymes of interest. In Aim
1, I will use this approach toward OH/MeO-BDE-producing red algae to establish, for the first time, the molecular
basis for PBDE biosynthesis in an eukaryotic system. Differential expression analysis under low and high
producing conditions will be employed to facilitate the identification of putative biosynthetic genes. In Aim 2,
known bacterial genes and newly identified eukaryotic genes will be used as probes to assess metagenomic
data from sea water, sediment, and marine animal associated microbes for the presence of PBDE biosynthesis
genes. Finally, the enzymology of PBDE biosynthetic enzymes will be explored in Aim 3 to provide a detailed
understanding of the underlying mechanisms of PBDE biosynthesis, specifically in the unique flavin dependent
halogenases responsible for aromatic decarboxylative bromination. I anticipate that this research will provide an
invaluable understanding of PBDE biosynthesis and its global distribution. Furthermore, this research will likely
facilitate studies to manage PBDE production in the environment and provide new insights into understudied
classes of enzymes.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
April Lukowski其他文献
April Lukowski的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('April Lukowski', 18)}}的其他基金
Interrogating novel biosynthetic sources for the production of polybrominated diphenyl ethers
探究生产多溴二苯醚的新型生物合成来源
- 批准号:
10673973 - 财政年份:2021
- 资助金额:
$ 6.76万 - 项目类别:
Interrogating novel biosynthetic sources for the production of polybrominated diphenyl ethers
探究生产多溴二苯醚的新型生物合成来源
- 批准号:
10313961 - 财政年份:2021
- 资助金额:
$ 6.76万 - 项目类别:
Biocatalytic approaches to antiepileptic drug targets
抗癫痫药物靶标的生物催化方法
- 批准号:
9922670 - 财政年份:2019
- 资助金额:
$ 6.76万 - 项目类别:
Biocatalytic approaches to antiepileptic drug targets
抗癫痫药物靶标的生物催化方法
- 批准号:
9761129 - 财政年份:2019
- 资助金额:
$ 6.76万 - 项目类别:
相似海外基金
ERI: Characterizing and improving algae-derived biofuel droplet burning
ERI:表征和改善藻类生物燃料液滴燃烧
- 批准号:
2301490 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Standard Grant
North Carolina Center for Coastal Algae, People, and Environment NC-CAPE
北卡罗来纳州沿海藻类、人类和环境中心 NC-CAPE
- 批准号:
2414792 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Continuing Grant
Investigating biosynthesis of the newly discovered natural product euglenatide and distribution across the breadth of Euglenoid algae
研究新发现的天然产物眼虫肽的生物合成及其在眼虫类藻类中的分布
- 批准号:
EP/Y003314/1 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Research Grant
Pathogens of Algae for Biocontrol and Biosecurity
用于生物防治和生物安全的藻类病原体
- 批准号:
EP/Y036808/1 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Research Grant
Molecular fossils, mass extinctions and the rise of complex algae
分子化石、大规模灭绝和复杂藻类的兴起
- 批准号:
DP240100281 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Discovery Projects
CORAL:Compostable Foams from Renewable Algae Sources: development and identification of strategies for their implementation
珊瑚:可再生藻类来源的可堆肥泡沫:制定和确定其实施策略
- 批准号:
EP/Y027701/1 - 财政年份:2024
- 资助金额:
$ 6.76万 - 项目类别:
Fellowship
Species identification, ecological elucidation, and resource value assessment of filamentous fungi parasitic on terrestrial plants and algae in polar regions
极地陆生植物和藻类寄生丝状真菌的物种鉴定、生态解析及资源价值评估
- 批准号:
23K11504 - 财政年份:2023
- 资助金额:
$ 6.76万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Carbon sequestration and sustainable product manufacture by algae using a novel photo-bioreactor
使用新型光生物反应器通过藻类进行碳封存和可持续产品制造
- 批准号:
2831630 - 财政年份:2023
- 资助金额:
$ 6.76万 - 项目类别:
Studentship
Elucidation of the molecular mechanisms driving the co-evolution between red tide-causing algae and viruses
阐明驱动赤潮藻类与病毒共同进化的分子机制
- 批准号:
23K14265 - 财政年份:2023
- 资助金额:
$ 6.76万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Unravelling the genetic basis of the gamete recognition system in brown algae
揭示褐藻配子识别系统的遗传基础
- 批准号:
23K19386 - 财政年份:2023
- 资助金额:
$ 6.76万 - 项目类别:
Grant-in-Aid for Research Activity Start-up