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Single-cell dissection of chromatin architecture mechanisms connecting pathologic instability and transcriptional silencing

连接病理不稳定和转录沉默的染色质结构机制的单细胞解剖

基本信息

批准号：
10473778
负责人：
Rajan Jain
金额：
$ 62.87万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-30 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10473778
关键词：
3-Dimensional Affect Amyotrophic Lateral Sclerosis Architecture Biological Assay Biological Models Carbon Cardiac Cardiac Myocytes Cardiomyopathies Cell Nucleus Cells Chromatin Chromatin Structure Clustered Regularly Interspaced Short Palindromic Repeats CpG Islands DNMT3a Data Disease Dissection Engineering Epigenetic Process Etiology Event Exhibits Exons FMR1 Fibrosis Fragile X Syndrome Friedreich Ataxia Functional disorder Gene Expression Gene Silencing Genes Genetic Transcription Genome Genome engineering Genomic Instability Genomics Heterochromatin Hi-C Higher Order Chromatin Structure Human Human Genome Hybrids Hydrophobicity Hypertrophy Image Individual Intercistronic Region Introns Knowledge Length Light Link Maps Mediating Mutation Neurons Nuclear Onset of illness Pathogenicity Pathologic Pathology Patients Pattern Peptides Peripheral Physiological Population Positioning Attribute RNA Radial Reporting Repression Resolution Short Tandem Repeat Somatic Cell Technology Testing Tissues Trinucleotide Repeat Expansion Variant Work base body system cell type cellular imaging chromosome conformation capture cohort density genome-wide genome-wide analysis genomic data genomic locus human disease induced pluripotent stem cell induced pluripotent stem cell derived cardiomyocytes insight mortality novel therapeutics single molecule single-cell RNA sequencing stem

项目摘要

Short tandem repeat regions (STR) are distributed evenly across the human genome, and recent genome-wide studies have demonstrated that STRs are polymorphic across individuals and linked to gene expression levels. STR instability at key genomic loci has been causally linked to disease pathophysiology in a range of expansion disorders. We recently demonstrated that nearly all disease-associated STRs co-localize with boundaries demarcating topologically associated domains (TADs). Moreover, we have observed that pathologic STR instability and transcriptional silencing can destroy the associated boundary and shift genomic loci to the nuclear periphery. These results now open critical unanswered questions regarding whether and how STR expansion and pathologic alterations in gene expression are functionally linked to boundary integrity and radial positioning. Here, we focus on the prototypic repeat expansion disorder Friedreich’s ataxia (FRDA) in which expansion of a GAA STR in the first intron of the FRATAXIN (FXN) gene results in cardiac and neuronal pathology. The cardiac pathology, specifically hypertrophy, fibrosis, and occasional dilation of the ventricle, is the etiology of significant FRDA mortality. GAA expansion is associated with the silencing of FXN transcription and a repositioning of the locus to the nuclear periphery. However, it remains unclear if the change in genome folding, radial positioning, or reduced expression drives STR expansion or vice versa. A major technical barrier contributing to this knowledge gap is that STR instability and genome folding are classically evaluated in bulk populations, however they exhibit tremendous variation across individual somatic cells of the same subtype and among cell types within a pathologically affected tissue. Here, we seek to decipher the causal link among STR instability, transcription, radial positioning, and genome folding. Our central hypothesis is that disruption of long-range loops is the initial event triggered by STR expansion leading to a cascade of heterochromatin spreading, silencing, and loss of radial positioning. We will test our hypothesis by generating genome-wide, single-cell maps of chromatin accessibility, expression, and the repressive H3K9me3 heterochromatin mark in GAA-expanded and control iPS cells and iPS-derived cardiomyocytes. We will integrate genomics data with single-cell sequential Oligopaints/OligoSTORM imaging of TADs and local chromatin structure, as well as single molecule RNA FISH for FXN expression. We will implement multiple genome engineering strategies, including dCas9-VP64 FXN activation and dCas9-CTCF loop re-engineering in FRDA GAA-iPS cells, and dCas9-Krab-Dnmt3a FXN silencing and dCas9-Krab CTCF-mediated loop disruption in healthy iPS cells. We will assay the effect of genome engineering approaches on TADs, radial positioning, STR length, and FXN expression in single cells. Successful completion of the proposed work will shed light on the pathophysiological mechanisms underlying repeat expansion disorders by deciphering the cause-and-effect relationships among genome folding, radial positioning, transcription, and STR expansion.

短串联重复区域（STR）在人类基因组中均匀分布，最近在全基因组中分布