Joint Cell Isolation Core of the MARC

联合细胞分离核心摘要 关节炎资源中心(MARC)微环境的联合细胞分离(JCI)核心将促进 圣地亚哥社区的研究集中在组织和单细胞水平上的关节生物学。这个 JCI Core将作为激光捕获显微切割(LCM)和Flow等技术的本地资源 从关节组织中进行分类。圣地亚哥地区没有其他共享资源提供这种类型的专业知识或 服务。JCI核心将由在应用程序方面具有丰富经验的教职员工领导和管理 这些技术用于风湿病的研究。核心将提供一系列运营商- 为人类和小鼠关节组织的LCM、组织解聚和血流分选提供辅助服务。这个 CORE还将帮助进行实验设计，与研究人员合作，根据需要定制方案 个人研究人员，并从事自己的内部研究，以开发新的尖端方法 对联合微环境进行调查。这个核心的目的是1)协助调查人员 从人类或小鼠关节分离组织碎片和/或单个细胞的实验设计，2)辅助 从关节组织学中分离原位细胞的实验研究人员 通过LCM的部分，以及3)协助调查人员执行涉及解聚 关节组织和特定细胞群体的分类。核心还将促进与其他 共享资源，以帮助调查人员满足与这些实验的工作流程相关的需求，例如 获取滑膜或软骨样本，组织学准备，并获得符合以下条件的样本 可以通过转录或染色质可及性等其他技术进行分析。通过提供服务 和专业知识，MARC JCI核心将使当地社区的研究人员能够使用以前的技术 对他们来说是不可用的，并将为他们的研究计划增加一个新的维度。

JOINT CELL ISOLATION CORE ABSTRACT The Joint Cell Isolation (JCI) Core of the Microenvironment in Arthritis Resource Center (MARC) will facilitate research in the San Diego community focused on the biology of the joint at the tissue and single cell level. The JCI Core will serve as a local resource for technologies such as laser capture microdissection (LCM) and flow sorting from joint tissues. No other shared resource in the San Diego area offers this type of expertise or service. The JCI Core will be led and managed by faculty and staff with extensive experience in the application of these technologies to research on rheumatologic diseases. The Core will provide a series of operator- assisted services for LCM, tissue disaggregation, and flow sorting from human and mouse joint tissues. The Core will also help with experimental design, collaborate with investigators to tailor protocols to the needs of the individual researcher, and engage in its own internal research to develop new cutting-edge methodologies for investigation into the joint microenvironment. The Aims of this Core are 1) to assist investigators in the design of experiments to isolate tissue fragments and/or single cells from human or mouse joints, 2) to assist investigators in the execution of experiments involving isolation of cells in situ from joint tissue histologic sections via LCM, and 3) to assist investigators in the execution of experiments involving disaggregation of joint tissue and sorting of specific cellular populations. The Core will also facilitate interactions with other shared resources to help investigators with needs related to the workflow for these experiments, such as procurement of synovium or cartilage samples, histological preparation of tissues, and obtaining samples that can be analyzed by transcriptomics or other technologies like chromatin accessibility. By providing services and expertise, the MARC JCI Core will enable researchers in the local community to use techniques previously unavailable to them and will add a new dimension to their research programs.