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Joint Cell Isolation Core of the MARC

MARC 的联合细胞分离核心

基本信息

批准号：
10472685
负责人：
Stephanie Stanford
金额：
$ 16.34万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10472685
关键词：
Antibodies Area Arthritis Bioinformatics Biology Biopsy Blood Vessels Books California Cartilage Cell Separation Cells Cellular biology Chondrocytes Chromatin Collection Communities Core Facility Custom Data Data Analyses Diffuse Dimensions Epigenetic Process Equipment Experimental Designs Faculty Fibroblasts Flow Cytometry Genetic Transcription Genomics Goals Histologic Human Human Resources Immunofluorescence Immunologic Immunology In Situ Individual Inflammation Inflammatory Infiltrate Investigation Joints Lasers Ligaments Lymphoid Mass Spectrum Analysis Medicine Methodology Microscope Mus Myeloid Cells National Institute of Arthritis and Musculoskeletal and Skin Diseases Nucleic Acids Pathogenesis Pathogenicity Pathology Population Procedures Protein Analysis Protocols documentation Research Research Personnel Resource Sharing Resources Rheumatism Rheumatoid Arthritis Rheumatology Sampling Science Scientist Secure Series Services Sorting - Cell Movement Specimen Structure Synovial Cell Synovial Membrane Synovitis T-Lymphocyte Techniques Technology Tissue Procurements Tissue Sample Tissue Stains Tissues Training base biobank bone design disease mechanisms study epigenomics experience experimental study human tissue interest laser capture microdissection power analysis preservation programs research and development tissue preparation transcriptome transcriptomics ultraviolet

项目摘要

JOINT CELL ISOLATION CORE ABSTRACT The Joint Cell Isolation (JCI) Core of the Microenvironment in Arthritis Resource Center (MARC) will facilitate research in the San Diego community focused on the biology of the joint at the tissue and single cell level. The JCI Core will serve as a local resource for technologies such as laser capture microdissection (LCM) and flow sorting from joint tissues. No other shared resource in the San Diego area offers this type of expertise or service. The JCI Core will be led and managed by faculty and staff with extensive experience in the application of these technologies to research on rheumatologic diseases. The Core will provide a series of operator- assisted services for LCM, tissue disaggregation, and flow sorting from human and mouse joint tissues. The Core will also help with experimental design, collaborate with investigators to tailor protocols to the needs of the individual researcher, and engage in its own internal research to develop new cutting-edge methodologies for investigation into the joint microenvironment. The Aims of this Core are 1) to assist investigators in the design of experiments to isolate tissue fragments and/or single cells from human or mouse joints, 2) to assist investigators in the execution of experiments involving isolation of cells in situ from joint tissue histologic sections via LCM, and 3) to assist investigators in the execution of experiments involving disaggregation of joint tissue and sorting of specific cellular populations. The Core will also facilitate interactions with other shared resources to help investigators with needs related to the workflow for these experiments, such as procurement of synovium or cartilage samples, histological preparation of tissues, and obtaining samples that can be analyzed by transcriptomics or other technologies like chromatin accessibility. By providing services and expertise, the MARC JCI Core will enable researchers in the local community to use techniques previously unavailable to them and will add a new dimension to their research programs.

关节细胞分离核心摘要关节炎资源中心（MARC）微环境的联合细胞分离（JCI）核心将促进圣地亚哥社区的研究集中在组织和单细胞水平的关节生物学上。的 JCI Core将作为激光捕获显微切割（LCM）和流动等技术的本地资源从关节组织中分离出来圣地亚哥地区没有其他共享资源提供这种类型的专业知识，的服务. JCI核心将由具有丰富应用经验的教职员工领导和管理应用这些技术来研究风湿性疾病。核心将提供一系列的运营商- 用于LCM、组织解聚和来自人类和小鼠关节组织的流式分选的辅助服务。的核心还将帮助实验设计，与研究人员合作，根据需要定制方案，并从事自己内部研究，以开发新的尖端方法研究关节微环境。本核心的目的是：1）协助调查人员从人或小鼠关节分离组织碎片和/或单细胞的实验设计，2）协助研究人员在进行涉及从关节组织组织学原位分离细胞的实验时，部分通过LCM，和3）协助研究人员在执行实验，涉及解聚关节组织和特定细胞群的分选。核心还将促进与其他共享资源，以帮助研究者满足与这些实验的工作流程相关的需求，例如滑膜或软骨样品的获取，组织的组织学制备，以及获得可以通过转录组学或其他技术如染色质可及性进行分析。通过提供服务和专业知识，MARC JCI核心将使当地社区的研究人员能够使用以前的技术，这将为他们的研究计划增加一个新的层面。