High-throughput engineering of ligand-selective fluorescent biosensors for detecting endogenous and exogenous opioids
用于检测内源性和外源性阿片类药物的配体选择性荧光生物传感器的高通量工程
基本信息
- 批准号:10635413
- 负责人:
- 金额:$ 251.02万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-04-15 至 2026-04-14
- 项目状态:未结题
- 来源:
- 关键词:AccelerationAdaptive BehaviorsAddressAffinityAnxietyAreaBRAIN initiativeBehaviorBehavioralBenchmarkingBiologyBiosensorBrainBrain regionCellsChimeric ProteinsCouplingDataDetectionDevelopmentDiseaseDrug ScreeningEndorphinsEngineeringEnhancersEnkephalinsEnsureFiberFluorescence MicroscopyFutureGeneticGoalsGreen Fluorescent ProteinsIn VitroInvertebratesKnock-outLearningLibrariesLigand BindingLigandsLinkLocationMeasurementMemoryMethodsModelingMolecularMolecular ConformationMonitorMusMutagenesisNeuronsNeuropeptidesNeurosciences ResearchNucleus AccumbensOpioidOpioid PeptideOpioid ReceptorOpticsOrganismPainPartner in relationshipPathologicPerformancePhotometryPhysiologicalPopulationPositioning AttributePropertyProtein EngineeringProteinsRandomizedReporterResearchResearch PersonnelSignal TransductionSliceSpecificitySpeedStressStructure of nucleus infundibularis hypothalamiTestingTimeVariantVertebratesWorkattenuationbiophysical propertiescell typecombatdelta opioid receptordesignendogenous opioidsexperimental studyfeedinghigh throughput screeningimprovedin vivoin vivo monitoringinnovationinnovative technologiesmu opioid receptorsneuronal circuitryneurotransmissionnext generationnovelnovel therapeutic interventionopioid abuseopioid withdrawaloptogeneticspharmacologicprototypereceptorsensortemporal measurementtool developmenttwo-photon
项目摘要
PROJECT SUMMARY / ABSTRACT
Neuropeptide modulation of neuronal circuits is strongly linked to many crucial behaviors such as
exploration, stress, memory formation, learning, and many pathophysiological conditions. Unfortunately,
neuropeptides are notoriously difficult to understand because many methods are not well-positioned to isolate
neuropeptide function accurately in space and time within the brain. Genetically-encoded fluorescent protein
sensors could provide precise monitoring with high-spatial and temporal resolution and cell-type specificity.
However, a significant obstacle in the engineering of neuropeptide sensors is the slow throughput of current
engineering approaches. Our central goal in this proposal is to develop advanced sensors specifically for
monitoring opioid neuropeptides dynamics in vivo by achieving large signal amplitudes and physiological-
relevant ligand binding affinities. At the same time, we will establish an efficient framework for neuropeptide
sensor engineering. We will utilize our new engineering platform to screen thousands of sensor variants in a few
minutes and with high efficiency. We will rapidly identify sensors with the required amplitudes and sensitivities
for circuit-specific opioid detection in vivo. Furthermore, we will characterize all sensors in models of evoked
endogenous opioid release in the brain of behaving mice. We have already engineered an opioid sensor
prototype with improved biophysical properties that we will use as a threshold in these paradigms. Our objective
is to generate multiple, specific sensors for advanced detection capabilities in neuronal circuits with a known
presence of opioid receptors and/or peptides. In Aim 1, we will create large sensor variant libraries to increase
signal amplitudes to combat the anticipated signal attenuation in in vivo applications. We will target specific
residues with randomized mutagenesis to facilitate the transition of sensor populations into active conformations.
Additionally, we will increase allosteric coupling between opioid sensing and reporter domains. In Aim 2, we will
generate sensors with specific ligand-selectivity profiles, e.g. enkephalin over endorphin, etc. We will generate
libraries targeting residues in or near the ligand-binding pocket. We will apply multiple ligands during our high-
throughput screens to identify sensors with the desired ligand-selectivity. In Aim 3, we will validate our sensors
in vivo and during behaviors that evoke opioid release. That includes monitoring endogenous opioid peptide
dynamics using fiber photometry in various brain regions with cell-type and circuit-type specificity. This proposal
is significant because neuropeptides are critical modulators of neuronal activity, but their dynamic actions are
not well understood due to the lack of appropriate in vivo monitoring. Our project is innovative because the
proposed approach will provide the fastest throughput in designing highly efficient neuropeptide sensor proteins.
In addition, opioid sensors could be the keys to identify neuronal mechanisms of state-dependent enhancers for
behaviors such as stress and anxiety or to probe brain circuits under conditions of opioid abuse.
项目摘要/摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Andre Berndt其他文献
Andre Berndt的其他文献
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{{ truncateString('Andre Berndt', 18)}}的其他基金
Next Generation Opto-GPCRs for Neuromodulatory Control
用于神经调节控制的下一代 Opto-GPCR
- 批准号:
10515612 - 财政年份:2023
- 资助金额:
$ 251.02万 - 项目类别:
Structure-guided and high-throughput engineering of genetically encoded sensors for reactive oxygen species
活性氧基因编码传感器的结构引导和高通量工程
- 批准号:
10092345 - 财政年份:2021
- 资助金额:
$ 251.02万 - 项目类别:
Structure-guided and high-throughput engineering of genetically encoded sensors for reactive oxygen species
活性氧基因编码传感器的结构引导和高通量工程
- 批准号:
10337219 - 财政年份:2021
- 资助金额:
$ 251.02万 - 项目类别:
In vivo real-time monitoring of reactive oxygen species and opioid signaling in a model for opioid receptor activity.
阿片受体活性模型中活性氧和阿片信号传导的体内实时监测。
- 批准号:
10369709 - 财政年份:2021
- 资助金额:
$ 251.02万 - 项目类别:
Structure-guided and high-throughput engineering of genetically encoded sensors for reactive oxygen species
活性氧基因编码传感器的结构引导和高通量工程
- 批准号:
10797426 - 财政年份:2021
- 资助金额:
$ 251.02万 - 项目类别:
Structure-guided and high-throughput engineering of genetically encoded sensors for reactive oxygen species
活性氧基因编码传感器的结构引导和高通量工程
- 批准号:
10551906 - 财政年份:2021
- 资助金额:
$ 251.02万 - 项目类别:
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