Novel polymeric materials with improved durability in the oral environment: tailoring responses to host and bacterial enzymes with anti-proteolytic and ecology-based antimicrobial approaches.

在口腔环境中具有更高耐用性的新型聚合物材料：通过抗蛋白水解和基于生态的抗菌方法定制对宿主和细菌酶的反应。

• 批准号: 10653700
• 负责人: Carmem S. Pfeifer
• 金额: $ 143.74万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10653700
• 关键词: Adhesives; Biocompatible Materials; Cementation; Clinical; Cohort Studies; Collagen; Composite Resins; Crowns; Dental; Dental caries; Ecology; Environment; Enzymes; Esthetics; Fracture; General Population; Healthcare; Hybrids; Hydrolysis; Longevity; Materials Testing; Matrix Metalloproteinases; Methacrylates; Microbial Biofilms; Oral; Oral cavity; Patients; Physiological; Plant Resins; Polymers; Procedures; Public Health; Recurrence; Resistance; Retreatment; Risk; Saliva; Structure; Surface; System; Time; Tissues; Tooth structure; Underserved Population; Water; aging population; antimicrobial; collagenase; composite restoration; design; dysbiosis; esterase; improved; inhibitor; mechanical load; methacrylamide; monomer; novel; oral bacteria; preservation; prevent; response; restoration

Dental caries continues to be a public health issue, especially more evident in underserved populations throughout the U.S. Unfortunately, especially with an ageing population, hundreds of thousands of resin composite restorations are replaced each year due to recurring decay and fracture. According to a number of cohort studies, the average life-span of this type of restoration is 10 years or less, depending on the caries risk level of the patient and on the complexity of the restorative procedure. This proposal represents an effort to develop novel antimicrobial structures, based on a targeted approach specifically against dysbiotic biofilm. Bacterial coalescence will be prevented by using GTF inhibitors, made polymerizable by functionalization with stable methacrylamides. In addition, MMP-inhibiting moieties will be added to a polymerizable monomer, improving the stability of the collagen in the hybrid layer. Ultimately, this will save millions of dollars annually and the unnecessary loss of additional tooth structure that comes with every re-treatment. The proposed approach will improve the longevity of restorations by: 1. Designing monomers containing known GTF-inhibiting moieties, thus making them available non-transiently at the surface of restorations. 2.Utilizing polymerizable functionalities that depart from the water/esterase-labile methacrylates. Methacrylamides are well known for their resistance to degradation by hydrolysis, and the systems proposed here will also be stable to enzymatic attack. 3. Incorporating MMP-inhibiting moieties on the methacrylamide monomer to the used as the adhesive, thus preserving the integrity of the collagen long-term. 4. Testing the materials in a physiologically relevant environment, mimicking the conditions in the mouth in terms of mechanical loading, bacterial challenge and presence of saliva.

龋齿仍然是一个公共卫生问题，特别是在服务不足的地区， 不幸的是，特别是随着人口老龄化，数百人 由于反复腐烂，每年要更换成千上万的树脂复合材料， 骨折根据一些队列研究，这类人的平均寿命 修复期为10年或更短，取决于患者的龋齿风险水平和 恢复性程序的复杂性。该提案代表了一种努力， 抗微生物结构，基于专门针对微生态生物膜的靶向方法。 细菌聚结将通过使用GTF抑制剂来防止，所述GTF抑制剂通过 用稳定的甲基丙烯酰胺官能化。此外，将添加MMP抑制部分， 与可聚合单体结合，从而提高混合层中胶原的稳定性。 最终，这将每年节省数百万美元，并减少不必要的额外损失。 每次再治疗都会带来的牙齿结构。建议的方法将改善 长寿的预防措施：1。设计含有已知GTF抑制部分的单体，因此 使得它们在双折射表面上非瞬时地可用。2.利用可聚合 这些官能团与水/酯酶不稳定的甲基丙烯酸酯不同。甲基丙烯酰胺 已知它们对水解降解的抗性，并且这里提出的系统也将是 对酶攻击稳定。3.在甲基丙烯酰胺单体上添加MMP抑制部分 用作粘合剂，从而长期保持胶原蛋白的完整性。4.测试 将材料置于生理相关环境中，模拟口腔中的条件， 根据机械负荷、细菌挑战和唾液的存在。

项目成果

Effect of different composite modulation protocols on the conversion and polymerization stress profile of bulk-filled resin restorations.

不同复合调制方案对整体填充树脂修复体的转化和聚合应力分布的影响。

• DOI: 10.1016/j.dental.2020.03.019
• 发表时间: 2020
• 期刊: Dental materials : official publication of the Academy of Dental Materials
• 作者: Erhardt,MCG;Goulart,M;Jacques,RC;Rodrigues,JA;Pfeifer,CS
• 通讯作者: Pfeifer,CS

The potential use of glycosyl-transferase inhibitors for targeted reduction of S. mutans biofilms in dental materials.

• DOI: 10.1038/s41598-023-39125-2
• 发表时间: 2023-07-23
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Scaffa, Polliana Mendes Candia;Kendall, Alexander;Icimoto, Marcelo Yudi;Fugolin, Ana Paula Piovezan;Logan, Matthew G.;DeVito-Moraes, Andre G.;Lewis, Steven H.;Zhang, Hua;Wu, Hui;Pfeifer, Carmem S.
• 通讯作者: Pfeifer, Carmem S.

Incorporation of Apigenin and tt-Farnesol into dental composites to modulate the Streptococcus mutans virulence.

• DOI: 10.1016/j.dental.2020.12.005
• 发表时间: 2021-04
• 期刊: Dental materials : official publication of the Academy of Dental Materials
• 作者: André CB;Rosalen PL;Giannini M;Bueno-Silva B;Pfeifer CS;Ferracane JL
• 通讯作者: Ferracane JL