Epidermal Gene Regulation by Transcription Elongation and Termination
通过转录延伸和终止调节表皮基因
基本信息
- 批准号:10698015
- 负责人:
- 金额:$ 34.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-09-11 至 2025-08-31
- 项目状态:未结题
- 来源:
- 关键词:Basal cell carcinomaBindingC-terminalCRISPR interferenceCandidate Disease GeneCellsChIP-seqChimeric ProteinsCleavage And Polyadenylation Specificity FactorCo-ImmunoprecipitationsComplexCoupledDNA Polymerase IIData SetDevelopmentDifferentiated GeneDifferentiation and GrowthDiseaseEpidermisEpitheliumEquilibriumFutureGene ExpressionGene Expression RegulationGenesGenetic TranscriptionGenomic SegmentGenomicsGlobal ChangeGoalsHomeostasisHumanImpairmentIndividualKnowledgeMaintenanceMalignant NeoplasmsMass Spectrum AnalysisMonitorNatural regenerationPathogenesisPatternPhosphorylationPlayPoly APolyadenylationPositive Transcriptional Elongation Factor BProcessProliferatingProteinsProteomicsPsoriasisRNARNA InterferenceRegulator GenesResearchRoleSerineSignal TransductionSiteSquamous cell carcinomaTestingTissue DifferentiationTissue ModelTissuesTranscription ElongationTranscription InitiationTranscription RepressorTranscriptional Elongation FactorsUndifferentiatedWorkchronic wounddesigndifferential expressiondosageepidermal stem cellgene functiongene producthuman diseasehuman tissueinhibitorinsightkeratinocytekeratinocyte differentiationknock-downnovelnovel therapeuticsprematurerecruitscaffoldskin disordertissue regenerationtranscription terminationtranscriptome sequencingwound healing
项目摘要
Project Summary
The long-term goal of this project is to elucidate the fundamental gene regulatory mechanisms controlling
epidermal tissue homeostasis. Thousands of genes are differentially expressed between undifferentiated and
differentiated keratinocytes, yet our current understanding of the gene regulatory mechanisms still remain
incomplete. In the context of transcription, which is the first and crucial stage of gene expression, a lot of efforts
have been concentrated on the first transcription initiation step. The roles of two other transcription steps,
namely the elongation and termination steps, remain largely unexplored. Therefore, this research plan is
designed to bridge this knowledge gap by characterizing the regulatory roles of transcription elongation and
termination processes in controlling epidermal gene expression.
Our preliminary characterization revealed that undifferentiated and differentiated keratinocytes acquire
differential expression patterns of transcription elongation and termination regulators. Leveraging our expertise
on genomics, proteomics, and human tissue models, we will determine how transcription elongation and
termination regulators cooperate with their interacting proteins to differentially regulate the elongation and
termination processes to control epidermal progenitor maintenance versus terminal tissue differentiation.
Dysregulation of epidermal gene expression underlies the pathogenesis of a spectrum of human skin
diseases including psoriasis, chronic wound healing, and cancer. Increased understanding of gene regulation
controlled by these under-characterized elongation and termination processes can generate novel therapeutic
ideas for treating human skin diseases.
项目摘要
该项目的长期目标是阐明控制基因表达的基本基因调控机制。
表皮组织稳态成千上万的基因在未分化和未分化之间差异表达。
分化的角质形成细胞,但我们目前对基因调控机制的理解仍然存在
不完整在转录的背景下,这是基因表达的第一个也是关键的阶段,
已经集中在第一转录起始步骤上。另外两个转录步骤的作用,
即延伸和终止步骤,在很大程度上仍未被探索。因此,该研究计划是
旨在通过表征转录延伸的调节作用来弥合这一知识差距,
终止过程控制表皮基因表达。
我们的初步表征表明,未分化和分化的角质形成细胞获得
转录延伸和终止调节子的差异表达模式。利用我们的专业知识
在基因组学、蛋白质组学和人体组织模型上,我们将确定转录延伸和
终止调节因子与它们的相互作用蛋白质合作,以差异性地调节延伸,
终止过程以控制表皮祖细胞维持相对于终末组织分化。
表皮基因表达失调是一系列人类皮肤病发病机制的基础
包括牛皮癣、慢性伤口愈合和癌症的疾病。增加对基因调控的理解
通过这些未充分表征的延伸和终止过程来控制,
治疗人类皮肤病的想法。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Xiaomin Bao', 18)}}的其他基金
Epidermal Gene Regulation by Transcription Elongation and Termination
通过转录延伸和终止调节表皮基因
- 批准号:
10460273 - 财政年份:2020
- 资助金额:
$ 34.73万 - 项目类别:
Epidermal Gene Regulation by Transcription Elongation and Termination
通过转录延伸和终止调节表皮基因
- 批准号:
9887603 - 财政年份:2020
- 资助金额:
$ 34.73万 - 项目类别:
Epidermal Gene Regulation by Transcription Elongation and Termination
通过转录延伸和终止调节表皮基因
- 批准号:
10261355 - 财政年份:2020
- 资助金额:
$ 34.73万 - 项目类别:
Regulation of human skin homeostasis by histone arginine methylation regulators
组蛋白精氨酸甲基化调节剂对人类皮肤稳态的调节
- 批准号:
8249334 - 财政年份:2011
- 资助金额:
$ 34.73万 - 项目类别:
Regulation of human skin homeostasis by histone arginine methylation regulators
组蛋白精氨酸甲基化调节剂对人类皮肤稳态的调节
- 批准号:
8125807 - 财政年份:2011
- 资助金额:
$ 34.73万 - 项目类别:
Regulation of human skin homeostasis by histone arginine methylation regulators
组蛋白精氨酸甲基化调节剂对人类皮肤稳态的调节
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8448767 - 财政年份:2011
- 资助金额:
$ 34.73万 - 项目类别:
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