Supplement for MINI point-of-use device

MINI 使用点设备的补充

• 批准号: 10656815
• 负责人: Christopher Edward Mason
• 金额: $ 17.02万
• 依托单位: UNIVERSITY OF MIAMI CORAL GABLES
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10656815
• 关键词: 2019-nCoV; Area; COVID-19; COVID-19 detection; COVID-19 outbreak; COVID-19 surveillance; COVID-19 testing; Collaborations; Computer software; Computers; Development; Devices; Disease Outbreaks; Evolution; Future; Genes; Infrastructure; Mediating; Medical; Methods; Monitor; Nucleic Acids; Pilot Projects; Process; RNase P; Risk Management; SARS-CoV-2 variant; Saliva; Sampling; Sewage; System; Technology; Testing; Time; Viral; Viral Envelope Gene; Virus; detection platform; isothermal amplification; pandemic disease; pathogen; point of care; point of care testing; portability; preservation; tool

Abstract We propose portable, proven, point of care/surveillance devices for use in SARS-CoV-2 testing that expands upon the TINY (Tiny Isothermal Nucleic acid quantification sYstem) , which is called the MINI. These devices leverage LAMP (loop mediated isothermal amplification) technology developed by the Mason Lab in collaboration with technological developments from the Erickson lab to test up to 96 samples in real time. These devices can operate in areas without existing medical infrastructure with portability and high throughput, filling an existing gap in the current SARS-CoV-2 testing infrastructure. We also envision these devices as filling a necessary gap in COVID-19 surveillance, leveraging pilot studies in detecting SARS-CoV-2 and other viruses in sewage to monitor the evolution of the pandemic in the coming years and building an infrastructure to better monitor and predict future viral outbreaks. A 96 well version of our existing TINY detection device, called the MINI, will be deployed at large scale to develop surveillance and point of care testing for SARS-CoV-2 in wastewater, saliva, and other samples. This technology is accompanied by an easy to use software package for exporting and preserving results that can be installed on any computer. This technology is able to screen the most prevalent circulating COVID-19 variants by targeting the viral envelope gene (E) and RNAse P (RP) gene. These methods can help with the current COVID-19 outbreak and aid in future risk management and pathogen mapping.

抽象的 我们建议在SARS-COV-2测试中使用便携式，经过验证的护理/监视设备 扩展在微小的（小等热核酸定量系统）上，这称为 小型的。这些设备利用灯（循环介导的等温放大）技术由 梅森实验室与埃里克森实验室的技术发展合作，测试高达96 实时样本。这些设备可以在没有现有医疗基础设施的区域运行 便携性和高吞吐量，填补当前SARS-COV-2测试基础架构中的现有空白。 我们还设想这些设备填补了COVID-19监视的必要空白，并利用飞行员 检测SARS-COV-2和其他污水中其他病毒的研究以监测大流行的演变 在未来几年中，建立基础设施，以更好地监控和预测未来的病毒爆发。 我们现有的小型检测设备（称为Mini）的96井版本将大规模部署到 在废水，唾液和其他 样品。该技术伴随着一个易于使用的软件包，用于导出和 保留可以在任何计算机上安装的结果。这项技术能够筛选最大的 通过靶向病毒包膜基因（E）和RNase P（RP）基因的流行循环covid-19变体。 这些方法可以帮助当前的共同爆发，并帮助未来的风险管理以及 病原体映射。

项目成果

Elevated A-to-I RNA editing in COVID-19 infected individuals.

• DOI: 10.1093/nargab/lqad092
• 发表时间: 2023-12
• 期刊: NAR genomics and bioinformatics
• 影响因子: 4.6

Highly host-linked viromes in the built environment possess habitat-dependent diversity and functions for potential virus-host coevolution.

• DOI: 10.1038/s41467-023-38400-0
• 发表时间: 2023-05-09
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Du, Shicong;Tong, Xinzhao;Lai, Alvin C. K.;Chan, Chak K.;Mason, Christopher E.;Lee, Patrick K. H.
• 通讯作者: Lee, Patrick K. H.

Machine learning algorithm to characterize antimicrobial resistance associated with the International Space Station surface microbiome.

• DOI: 10.1186/s40168-022-01332-w
• 发表时间: 2022-08-24
• 期刊: Microbiome
• 影响因子: 15.5