Supplement for MINI point-of-use device

摘要 我们提出了便携式、经过验证的、用于SARS-CoV-2检测的现场护理/监测设备， 扩展了TINY（微小等温核酸定量系统），该系统被称为 MINI.这些设备利用LAMP（环介导等温扩增）技术， 梅森实验室与埃里克森实验室的技术开发合作， 真实的时间。这些设备可以在没有现有医疗基础设施的地区运行， 便携性和高吞吐量，填补了目前SARS-CoV-2测试基础设施的现有空白。 我们还设想这些设备将填补COVID-19监测的必要空白， 研究检测污水中的SARS-CoV-2和其他病毒，以监测大流行的演变 并建立基础设施，以更好地监测和预测未来的病毒爆发。 我们现有的TINY检测设备的96孔版本，称为MINI，将大规模部署， 开展废水、唾液和其他物质中SARS-CoV-2的监测和护理点检测 样品这项技术是伴随着一个易于使用的软件包， 保存可以安装在任何计算机上的结果。这项技术能够筛选最 通过靶向病毒包膜基因（E）和RNA酶P（RP）基因， 这些方法可以帮助应对当前的COVID-19疫情，并有助于未来的风险管理， 病原体定位

Abstract We propose portable, proven, point of care/surveillance devices for use in SARS-CoV-2 testing that expands upon the TINY (Tiny Isothermal Nucleic acid quantification sYstem) , which is called the MINI. These devices leverage LAMP (loop mediated isothermal amplification) technology developed by the Mason Lab in collaboration with technological developments from the Erickson lab to test up to 96 samples in real time. These devices can operate in areas without existing medical infrastructure with portability and high throughput, filling an existing gap in the current SARS-CoV-2 testing infrastructure. We also envision these devices as filling a necessary gap in COVID-19 surveillance, leveraging pilot studies in detecting SARS-CoV-2 and other viruses in sewage to monitor the evolution of the pandemic in the coming years and building an infrastructure to better monitor and predict future viral outbreaks. A 96 well version of our existing TINY detection device, called the MINI, will be deployed at large scale to develop surveillance and point of care testing for SARS-CoV-2 in wastewater, saliva, and other samples. This technology is accompanied by an easy to use software package for exporting and preserving results that can be installed on any computer. This technology is able to screen the most prevalent circulating COVID-19 variants by targeting the viral envelope gene (E) and RNAse P (RP) gene. These methods can help with the current COVID-19 outbreak and aid in future risk management and pathogen mapping.