Advanced Technology to Study Visual Function on a Cellular Scale

在细胞尺度上研究视觉功能的先进技术

• 批准号: 10661562
• 负责人: JACQUE LYNNE DUNCAN
• 金额: $ 106.06万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661562
• 关键词: Affect; Alabama; Animal Model; Animal Testing; Biomedical Engineering; Brain imaging; Cells; Clinic; Clinical; Color; Color Visions; Compensation; Cone; Development; Disease; Early Diagnosis; Engineering; Environment; Eye; Eye diseases; Funding; Grant; Human; Image; Individual; Investigation; Light; Macaca; Measurement; Measures; Metadata; Methods; Microscopic; Modality; Modeling; Morphology; Motion; Neurophysiology - biologic function; Optical Instrument; Pathway interactions; Patients; Perception; Perimetry; Photic Stimulation; Photoreceptors; Physiological; Physiology; Population; Progress Reports; Property; Psychophysics; Research; Resolution; Retina; Retinal Cone; Retinal Diseases; Retinitis Pigmentosa; Rod; San Francisco; Series; Shapes; Signal Transduction; Software Tools; Specific qualifier value; Specificity; Speed; Stimulus; Structure; System; Technology; Testing; Time; Translating; Translations; Validation; Vertebrate Photoreceptors; Vision; Vision Tests; Visual; Visual Cortex; Visual Perception; Visual System; Visualization; Work; adaptive optics; adaptive optics scanning laser ophthalmoscopy; base; cellular development; clinical application; clinical investigation; clinical translation; computerized tools; cone-rod degeneration; efficacy evaluation; experience; experimental study; fovea centralis; improved; in vivo; innovation; insight; instrument; knowledge translation; microstimulation; nanoscale; neural; new technology; nonhuman primate; optical imaging; optical switch; receptive field; response; retinal imaging; sensory input; spatial vision; spatiotemporal; tool; two-photon; vision science; visual control; visual learning; visual performance; visual processing; visual tracking

Project Summary We are asking for support to continue to develop and enhance three state-of-the-art optical instruments that will be used to answer questions about the most important and the most challenging region in the retina to study, the fovea. The instruments are built upon two key technical strengths - adaptive optics scanning laser ophthalmoscope (AOSLO) systems and accurate, high-speed eye-motion tracking. Adaptive optics technology corrects the imperfections in the eye and can be used to generate microscopic views of the living retina and deliver ultra-sharp images to the retina. Eye tracking is used to measure and compensate for ever-present eye motion. Together, these allow for visualization, tracking and delivery of light to retinal features as small as single cone photoreceptors, enabling measurements of properties of spatial and color vision on an unprecedented scale. Although the three systems will be identical, the scope of study for each system will be very different. The AOSLO at in Alabama will be used to test vision in non-human primates, the AOSLO in Berkeley will be used to perform advanced vision testing on healthy human eyes, and the AOSLO in San Francisco will be used to study patients with eye disease. The key advantage of having the BRP manage three identical systems is that it will facilitate hardware innovations plus rapid translation of knowledge and innovative testing from animal models to the clinic. Briefly, the specific aims are: Aim 1: Advanced AOSLO display capabilities for color vision: We propose a series of technical developments will expand the scope of AOSLO experiments, not just for color vision, but also spatial vision and clinical applications. Specifically, we will (i) add 2-photon stimulation (ii) develop new methods to display large stimuli that are fixed in world-coordinates (iii) integrate dichoptic displays to enable experiments that distinguish retinal from cortical visual processing (iv) develop I-TRACK (improved software tools for retina- contingent vision testing) and (v) invisible imaging and tracking. These tools will enable a series of experiments to learn how the visual system extracts color and spatial information from its sensory inputs. Aim 2: Enhanced AOSLO systems and modeling for spatial vision: In this aim we will (i) develop advanced wavefront propagation tools to model light-cone interactions (ii) integrate AOSLO microstimulation with a system for 2-photon functional brain imaging in non-human primates. We aim to use these tools to greatly enhance our understanding of receptive fields at and near the fovea. Aim 3: Clinical translation: We will integrate the new technology into the system at UCSF to (i) study rod vision in patients with rod-cone degenerations (ii) measure the time course, structure and function of dysflective cones (iii) investigate the structure and function of the preferred retinal locus in diseases that affect the fovea and (iv) assess inner retinal function in eye disease.

项目总结

项目成果

Alignment, calibration, and validation of an adaptive optics scanning laser ophthalmoscope for high-resolution human foveal imaging.

用于高分辨率人体中心凹成像的自适应光学扫描激光检眼镜的对准、校准和验证。

• DOI: 10.1364/ao.504283
• 发表时间: 2024
• 期刊: Applied optics
• 影响因子: 1.9
• 作者: Moon,Benjamin;Poletti,Martina;Roorda,Austin;Tiruveedhula,Pavan;Liu,SohHang;Linebach,Glory;Rucci,Michele;Rolland,JannickP
• 通讯作者: Rolland,JannickP

Characterizing Fixational Eye Motion Variance Over Time as Recorded by the Tracking Scanning Laser Ophthalmoscope.

• DOI: 10.1167/tvst.11.2.35
• 发表时间: 2022-02-01
• 期刊: Translational vision science & technology
• 影响因子: 3
• 作者: Condor Montes SY;Bennett D;Bensinger E;Rani L;Sherkat Y;Zhao C;Helft Z;Roorda A;Green AJ;Sheehy CK
• 通讯作者: Sheehy CK

Relationship Between Foveal Cone Structure and Visual Acuity Measured With Adaptive Optics Scanning Laser Ophthalmoscopy in Retinal Degeneration.

• DOI: 10.1167/iovs.17-23708
• 发表时间: 2018-07-02
• 期刊: Investigative ophthalmology & visual science
• 影响因子: 4.4
• 作者: Foote KG;Loumou P;Griffin S;Qin J;Ratnam K;Porco TC;Roorda A;Duncan JL
• 通讯作者: Duncan JL

Poster Session: Towards Understanding Retinal Processing of Single-Cone Scale Stimulation.

海报会议：了解单视锥尺度刺激的视网膜处理。

• 发表时间: 2023
• 期刊: Journal of vision
• 影响因子: 1.8
• 作者: Pirog,JT;Tuten,WilliamS
• 通讯作者: Tuten,WilliamS

Eye tracking: empirical foundations for a minimal reporting guideline.

• DOI: 10.3758/s13428-021-01762-8
• 发表时间: 2023-01
• 期刊: Behavior research methods
• 影响因子: 5.4
• 作者: Holmqvist K;Örbom SL;Hooge ITC;Niehorster DC;Alexander RG;Andersson R;Benjamins JS;Blignaut P;Brouwer AM;Chuang LL;Dalrymple KA;Drieghe D;Dunn MJ;Ettinger U;Fiedler S;Foulsham T;van der Geest JN;Hansen DW;Hutton SB;Kasneci E;Kingstone A;Knox PC;Kok EM;Lee H;Lee JY;Leppänen JM;Macknik S;Majaranta P;Martinez-Conde S;Nuthmann A;Nyström M;Orquin JL;Otero-Millan J;Park SY;Popelka S;Proudlock F;Renkewitz F;Roorda A;Schulte-Mecklenbeck M;Sharif B;Shic F;Shovman M;Thomas MG;Venrooij W;Zemblys R;Hessels RS
• 通讯作者: Hessels RS