Spirulina oral supplement for enhancing host resilience to virus infection

螺旋藻口服补充剂可增强宿主对病毒感染的抵抗力

• 批准号: 10683642
• 负责人: Ikhlas Ahmad Khan
• 金额: $ 20.03万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10683642
• 关键词: Advisory Committees; Animal Model; Antiviral Therapy; Biological Assay; Biological Markers; Botanical dietary supplements; Botanicals; Cessation of life; Chemicals; Collaborations; Communication; Data; Doctor of Philosophy; Dose; Ensure; Evaluation; Formulation; Future; Hospitalization; Host resistance; Human; Human Subject Research; Immune; Immune response; Immunology; Influenza; Intervention Studies; Knowledge; Legal patent; Lipoproteins; Maintenance; Mississippi; Molecular; Mus; Natural Products; Pathogenicity; Patients; Pharmacy (field); Postdoctoral Fellow; Recovery; Regimen; Reproducibility; Research; Research Personnel; Research Project Grants; Resource Allocation; Spirulina preparation; Standardization; Supplementation; Universities; Viral Respiratory Tract Infection; Virus Diseases; Work; base; career development; design; efficacy clinical trial; efficacy study; graduate student; human disease; human model; immune health; improved; influenza infection; meetings; member; multidisciplinary; oral supplementation; resilience; training opportunity; web site

OVERALL: ABSTRACT/SUMMARY The University of Mississippi (UM) Botanical Dietary Supplements Research Center (BDSRC) is focused on filling in knowledge gaps related to the potential for the Spirulina-based product, ImmulinaTM, to promote resilience against and/or recovery from influenza and, by extension, other respiratory viral infections. ImmulinaTM is a patented bioassay-standardized extract that concentrates the active immunostimulatory compounds in Spirulina, i.e., Braun-type lipoproteins. Researchers affiliated with the UM BDSRC have generated evidence that supports reproducible and mechanistically plausible effects of ImmulinaTM for enhancing host responses that may promote resilience against respiratory viral infections such as influenza. Last year in the U.S. alone, influenza resulted in 959,000 patient hospitalizations and 79,400 deaths. Under the direction of Ikhlas Khan, PhD and Nirmal Pugh, PhD, the UM BDSRC includes a well- established collaborative team of multi-disciplinary investigators with expertise in natural products, animal models of human diseases, pharmaceutics, immunology, and human subject research. The UM BDSRC will be composed of an Administration Core, a Botanical Core, and two research projects: (1) Unraveling Immune Enhancement by ImmulinaTM, and (2) Evaluation of ImmulinaTM Oral Supplement for Host Resistance to Influenza Virus Infection. The overall specific aims are to: (1) maximize coordination of effort, communication among researchers, training opportunities for career development, and dissemination of knowledge through a well-managed and effective Administrative Core; (2) ensure product integrity of the ImmulinaTM extract through a combination of bioassay- and chemical-based approaches for standardization within the Botanical Core; (3) determine the molecular mechanism of immune enhancement of ImmulinaTM and optimal formulation and dosing in Project 1’s mouse study that can be utilized by the UM BDSRC’s other studies; and (4) using both a mouse and subsequently a biomarker-based human model, establish the impact of ImmulinaTM supplementation on increasing host resilience against the pathogenic effects of influenza virus infection with Project 2. The Administrative Core, guided by an External Advisory Committee and an Internal Steering Committee, will oversee all aspects of the UM BDSRC, including progress towards meeting its milestones, efficient allocation of resources, collaborations with researchers outside the UM BDSRC, oversight of its graduate students and post-doctoral fellows, and maintenance of its website. Members of the Botanical Core will work closely with researchers from the UM BDSRC to provide sufficient quantities of standardized ImmulinaTM extract and to collaborate with other units of CARBON on future efficacy studies of ImmulinaTM. The results from Projects 1 and 2 will help establish the optimal regimen for future clinical efficacy trials.

总体：摘要/摘要 密西西比大学(UM)植物膳食补充剂研究中心(BDSRC) 填补与螺旋藻为基础的产品ImmulinaTM推广潜力相关的知识空白 对流感和其他呼吸道病毒感染的复原力和/或恢复力。 ImmulinaTM是一种获得专利的生物测定标准化提取物，浓缩了活性免疫刺激物质 螺旋藻中的化合物，即布劳恩型脂蛋白。隶属于密歇根大学BDSRC的研究人员已经 生成的证据支持ImmulinaTM的可重复性和机械上可信的效果 增强宿主的反应，从而提高对流感等呼吸道病毒感染的抵抗力。 去年仅在美国，流感就导致95.9万名患者住院，7.94万人死亡。 在博士Ikhlas Khan和博士Nirmal Pugh的指导下，UM BDSRC包括一口井- 建立了多学科合作调查团队，拥有天然产品、动物方面的专业知识 人类疾病模型、药剂学、免疫学和人类主题研究。UM BDSRC将是 由一个管理核心、一个植物学核心和两个研究项目组成：(1)解开免疫 (2)ImmulinaTM口服补充剂对白粉病寄主抗性的评价 流感病毒感染。 总的具体目标是：(1)最大限度地协调工作、研究人员之间的沟通、 为职业发展提供培训机会，并通过管理良好的 有效的管理核心；(2)通过以下组合确保ImmulinaTM萃取物的产品完整性 以生物测定和化学为基础的方法在植物核心内标准化；(3)确定 免疫调节剂免疫增强的分子机制及工程中最佳处方和剂量 1‘S小鼠研究，可用于密歇根大学北京交通安全研究中心的其他研究；以及(4)同时使用小鼠和 随后建立了基于生物标记物的人体模型，建立了ImmulinaTM对人体的影响 通过项目2提高宿主对流感病毒感染的致病效应的抵抗力。 行政核心由外部咨询委员会和内部指导委员会指导， 将监督UM BDSRC的所有方面，包括实现其里程碑的进展，高效 资源分配，与UM BDSRC以外的研究人员合作，监督其毕业生 学生和博士后研究员，并维护其网站。植物核心的成员将工作 与UM BDSRC的研究人员密切合作，提供足够数量的标准化ImmulinaTM 提取并与其他碳单位合作进行ImmulinaTM的未来疗效研究。结果是 来自项目1和项目2的研究将有助于为未来的临床疗效试验建立最佳方案。

项目成果

Histomorphometric lung density evaluation of Immulina treatment using a murine influenza pneumonia model.

• DOI: 10.1111/iep.12493
• 发表时间: 2023-12
• 期刊: International journal of experimental pathology
• 影响因子: 3

Immulina as an Immunostimulatory Supplement: Formulation and Pharmacological Studies.

Immulina 作为免疫刺激补充剂：配方和药理学研究。

• DOI: 10.1055/a-2156-4653
• 发表时间: 2023
• 期刊: Planta medica
• 影响因子: 2.7
• 作者: Li,Yusheng;Ji,Nan;Wang,Minia;Pugh,NirmalD;Khan,IkhlasA;Tan,Chalet
• 通讯作者: Tan,Chalet

Validation of a Toll-like Receptor (TLR)2/TLR1 Activation Assay for Biological Standardization of Arthrospira/Limnospira Immune-Enhancing Potency.

Toll 样受体 (TLR)2/TLR1 激活测定的验证，用于节旋藻/Limnospira 免疫增强效力的生物标准化。

• DOI: 10.1080/19390211.2023.2263566
• 发表时间: 2024
• 期刊: Journal of dietary supplements
• 影响因子: 2.5
• 作者: Haron,MonaH;Zhang,Jin;Chittiboyina,AmarG;Khan,IkhlasA;Pugh,NirmalD
• 通讯作者: Pugh,NirmalD

Utility of fatty acid profile and in vitro immune cell activation for chemical and biological standardization of Arthrospira/Limnospira.

脂肪酸谱和体外免疫细胞活化的效用，用于关节螺旋体/limnospira的化学和生物标准化。

• DOI: 10.1038/s41598-022-19590-x
• 发表时间: 2022-09-19
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Huh, Jungmoo;Zhang, Jin;Hauerova, Radka;Lee, Joseph;Haider, Saqlain;Wang, Mei;Hauer, Tomas;Khan, Ikhlas A.;Chittiboyina, Amar G.;Pugh, Nirmal D.
• 通讯作者: Pugh, Nirmal D.