Core C: Imaging Core

摘要 成像核心（核心C）将提供与样品处理、图像采集和图像处理相关的服务。 所有PPG研究者的分析。由于非常规的尺寸和复杂的聚合物组织， 粘蛋白，研究复杂的生物系统，如粘液，需要创新，可行，可靠 实验方法进行适当的调查。Core C提供富含粘液样本的专业知识 操作/处理/染色和使用广泛的图像采集技术熟练度 显微镜技术。通过核心C可获得的成像技术包括RNA原位杂交， 免疫组织化学、粘蛋白琼脂糖蛋白质印迹、扫描和透射电子显微镜，以及 原子力显微镜所有这些成像技术的组合将提供替代方法， 可视化粘膜纤毛器的关键组成部分，并深入了解分子相互作用 控制粘液的粘弹性。除了提供高质量的显微照片，Core C 协助调查人员使用各种软件进行最先进的图像分析，（例如，ImageJ，VisioPharm， Odyssey），以确保数字图像的准确定量分析。毫无疑问， 核心C依赖于Ehre实验室和现有显微镜设施之间多年建立的合作关系 (MSL和HIC）在校园里。这些充电中心及其现有设备的使用已被 在预算中仔细分配。核心C旨在提供方案、试剂以及 样品处理、图像采集和数字成像方面的专业知识、指导和技术援助 项目1、2、3和4的分析。

Abstract The Imaging Core (Core C) will provide services related to sample processing, image acquisition and image analysis for all PPG investigators. Due to the unconventional size and intricate polymeric organization of mucins, studying complex biological systems like mucus require innovative, workable, and reliable experimental approaches for proper investigations. Core C offers expertise in mucus-rich sample handling/processing/staining and technical proficiency for image acquisition using a broad range of microscopy techniques. Imaging techniques available through Core C include RNA-in situ hybridization, immunohistochemistry, mucin agarose Western blotting, scanning and transmission electron microscopy, and atomic force microscopy. The combination of all of these imaging techniques will provide alternative ways to visualization key components of the mucociliary apparatus and gain insights into the molecular interactions that govern the viscoelastic properties of mucus. In addition to providing high-quality micrographs, Core C assists investigators with state-of-the-art image analysis using various software, (e.g., ImageJ, VisioPharm, Odyssey) to ensure accurate quantitative analyses of digital images. Indubitably, the proper functioning of Core C relies on partnerships established for years between the Ehre lab and existing microscopy facilities (MSL and HIC) on UNC campus. The use of these recharge centers and their existing equipment has been carefully allocated in the budget section. Core C is designed to provide protocols, reagents, as well as expertise, guidance and technical assistance for sample processing, image acquisition and digital image analysis for projects 1, 2, 3, and 4.