Core C: Imaging Core

摘要 成像核心(核心C)将提供与样品处理、图像采集和图像有关的服务 对所有PPG调查人员的分析。由于非常规的尺寸和复杂的聚合物组织 粘蛋白，研究复杂的生物系统，如粘液，需要创新、可行和可靠 适当调查的实验方法。Core C提供富含粘液的样本方面的专业知识 处理/处理/染色和熟练使用广泛的图像采集 显微镜技术。Core C提供的成像技术包括RNA原位杂交， 免疫组织化学、粘蛋白琼脂糖凝胶印迹、扫描和透射电子显微镜观察 原子力显微镜。所有这些成像技术的组合将提供替代方法 可视化粘液纤毛器的关键组件并深入了解分子间的相互作用 它们支配着粘液的粘弹性性质。除了提供高质量的显微照片外，Core C 帮助调查人员使用各种软件(例如ImageJ、VisioPharm、 奥德赛)，以确保准确的数字图像的定量分析。毋庸置疑的是， CORE C依赖于EHRE实验室和现有显微镜设施之间多年建立的合作伙伴关系 (MSL和HIC)在北卡罗来纳大学校园。这些充值中心及其现有设备的使用一直是 在预算一节中仔细分配。核心C旨在提供协议、试剂以及 样品处理、图像采集和数字图像方面的专门知识、指导和技术援助 项目1、2、3和4的分析。

Abstract The Imaging Core (Core C) will provide services related to sample processing, image acquisition and image analysis for all PPG investigators. Due to the unconventional size and intricate polymeric organization of mucins, studying complex biological systems like mucus require innovative, workable, and reliable experimental approaches for proper investigations. Core C offers expertise in mucus-rich sample handling/processing/staining and technical proficiency for image acquisition using a broad range of microscopy techniques. Imaging techniques available through Core C include RNA-in situ hybridization, immunohistochemistry, mucin agarose Western blotting, scanning and transmission electron microscopy, and atomic force microscopy. The combination of all of these imaging techniques will provide alternative ways to visualization key components of the mucociliary apparatus and gain insights into the molecular interactions that govern the viscoelastic properties of mucus. In addition to providing high-quality micrographs, Core C assists investigators with state-of-the-art image analysis using various software, (e.g., ImageJ, VisioPharm, Odyssey) to ensure accurate quantitative analyses of digital images. Indubitably, the proper functioning of Core C relies on partnerships established for years between the Ehre lab and existing microscopy facilities (MSL and HIC) on UNC campus. The use of these recharge centers and their existing equipment has been carefully allocated in the budget section. Core C is designed to provide protocols, reagents, as well as expertise, guidance and technical assistance for sample processing, image acquisition and digital image analysis for projects 1, 2, 3, and 4.