Core 2 - Mucosal Immunobiology Core (MIC)

核心 2 - 粘膜免疫生物学核心 (MIC)

• 批准号: 10700081
• 负责人: Catherine Lozupone
• 金额: $ 35.68万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10700081
• 关键词: Address; Aerobic Bacteria; Anaerobic Bacteria; Bacteria; Cell Separation; Cell physiology; Cells; Collaborations; Colorado; Communities; Consultations; Data; Data Analyses; Databases; Development; Experimental Designs; Feces; Fee-for-Service Plans; Flow Cytometry; Gene Expression; Generations; Goals; Homeostasis; Human; Immune; Immunobiology; Immunology; In Vitro; Individual; Interdisciplinary Study; Intestinal Mucosa; Intestines; Investments; Libraries; Lower respiratory tract structure; Metabolic; Metagenomics; Methods; Microbe; Mucous Membrane; Nucleic Acids; Oral cavity; Organ; Pathogenesis; Pathway interactions; Phenotype; Population; Process; Production; Productivity; Research; Research Personnel; Research Support; Resources; Rheumatism; Rheumatoid Arthritis; Ribosomal RNA; Role; Sampling; Science; Services; Shotguns; Spondylarthritis; Sputum; Statistical Data Interpretation; Statistical Study; Technology; Tissues; Training; Universities; Validation; career; cell capsule; cell envelope; cervicovaginal; commensal microbes; data integration; density; design; diverse data; host-microbe interactions; human tissue; interest; lipidome; lipidomics; liquid chromatography mass spectrometry; metabolome; metabolomics; metatranscriptomics; method development; microbial; microbial community; microbial host; microbial products; microbiome; microbiota; novel; phenotypic data; response; small molecule; trafficking; transcriptome sequencing

Summary: Resource Core 2: Mucosal Immunobiology Core (MIC) Compounding evidence exists for the role of microbiota and mucosal immunology in the pathogenesis of rheumatic diseases including rheumatoid arthritis (RA) and spondyloarthritis (SpA). The Mucosal Immunobiology Core (MIC) aims to provide consultation, discounted services, and development of enhanced capabilities to support the prediction and validation of mechanisms of host:microbe interactions in biospecimens of importance to rheumatic disease pathogenesis. The MIC will facilitate collaboration between individuals with expertise in microbiome, metabolome/lipidome, immunology, statistical data analysis, and rheumatic disease, which is essential for conducting this multidisciplinary research. Taken together, this core will support research into host:microbe interactions through 1) assembling individuals with diverse and complimentary expertise for promoting team science approaches focused on mucosal phenotypes and systemic consequences; 2) educating young investigators and those new to the field; 3) providing discounted services; and 4) novel methods development. More specifically, the MIC will support discounted services for the characterization of the microbiome and metabolome of biospecimens and tissues of importance in rheumatic disease pathogenesis, including feces, sputum, oral cavity, lower respiratory tract, intestinal mucosa, and cervicovaginal mucosa. Since challenges in data analysis can often be a bottleneck in conducting productive research in mucosal immunobiology, the MIC will also support free and discounted services and training in integrated ‘omic data analysis. The MIC will also support experimental validation of microbe:metabolite:immune phenotype relationships via in vitro cell stimulations, by providing services, consultation, and support in the expansion of anaerobic and aerobic bacteria and flow cytometry and density gradient based isolation of bacteria, and isolation of immune cells of interest. The MIC will develop enhanced capabilities in use of RNASeq to characterize both host and microbial gene expression simultaneously in intestinal tissue and associated multi’omic integrated data analyses. The MIC will also develop enhanced capabilities in annotation of metabolomic data through the creation of tissue specific databases for annotation of untargeted LC/MS data from feces and sputum samples. Finally, the MIC will invest in developing novel methods for isolating cell populations of interest for use in in vitro interrogations of host:microbe interactions. By integrating individuals with diverse expertise and providing discounted services, training and support, the MIC will support both early career and established investigators in the University of Colorado (CU) Rheumatic Disease Research Resource Center (RDRRC), as well as assist investigators new to the field of rheumatic disease to enhance overall research implementation and productivity.

摘要：资源核心2：粘液素免疫生物学核心（MIC） 存在复合证据表明微生物群和粘膜免疫学在胃肠道疾病发病机制中的作用。 风湿性疾病，包括类风湿性关节炎（RA）和脊柱关节炎（SpA）。粘液免疫生物学 核心（MIC）旨在提供咨询，折扣服务和增强能力的发展， 支持预测和验证重要生物标本中宿主：微生物相互作用的机制 风湿病的发病机制。MIC将促进具有以下专业知识的个人之间的合作： 微生物组、代谢组/脂质组、免疫学、统计数据分析和风湿性疾病， 这是进行这项多学科研究的关键。总的来说，这个核心将支持研究， 宿主：微生物通过1）聚集具有不同和互补专业知识的个体， 促进团队科学方法，重点是粘膜表型和系统性后果; 2）教育 年轻的研究人员和那些新到外地; 3）提供折扣服务;和4）新的方法 发展更具体地说，MIC将支持折扣服务， 生物样本和组织的微生物组和代谢物组在风湿病发病机制中具有重要性， 包括粪便、痰、口腔、下呼吸道、肠粘膜和宫颈阴道粘膜。以来 数据分析中的挑战通常是在粘膜中进行生产性研究的瓶颈 此外，MIC还将支持免费和打折的服务以及综合组学数据的培训 分析. MIC还将支持微生物：代谢物：免疫表型的实验验证 通过体外细胞刺激，通过提供服务，咨询和支持， 厌氧和需氧细菌以及基于流式细胞术和密度梯度的细菌分离， 的免疫细胞。MIC将开发使用RNASeq的增强功能，以表征 肠道组织中宿主和微生物基因同时表达及相关多组学综合数据 分析。MIC还将开发增强的代谢组学数据注释能力， 创建组织特异性数据库，用于注释粪便和痰液样本的非靶向LC/MS数据。 最后，MIC将投资开发分离感兴趣的细胞群的新方法，以供体外使用。 询问宿主：微生物相互作用。通过整合具有不同专业知识的个人， 通过提供折扣服务、培训和支持，MIC将支持早期职业和已建立的调查人员 在科罗拉多大学（CU）风湿病研究资源中心（RDRRC），以及协助 研究人员新的风湿病领域，以提高整体研究的实施和生产力。