Core 2 - Mucosal Immunobiology Core (MIC)

摘要：资源核心2：粘膜免疫生物学核心(MIC) 已有复合证据表明微生物区系和粘膜免疫学在肺炎发病机制中的作用。 风湿性疾病包括类风湿性关节炎(RA)和脊柱关节炎(SpA)。粘膜免疫生物学 核心(MIC)旨在提供咨询、折扣服务和增强能力的开发，以 支持宿主机制的预测和验证：微生物相互作用在生物谱系中的重要性 对风湿病病机的认识。MIC将促进具有以下专业知识的个人之间的合作 微生物组、代谢组/脂质组、免疫学、统计数据分析和风湿病，这是 对于进行这种多学科的研究是必不可少的。总而言之，这个核心将支持对 主持人：通过1)集合具有多样化和互补性专业知识的人员进行微生物互动 促进团队科学方法，侧重于粘膜表型和系统后果；2)教育 青年研究人员和新手；3)提供折扣服务；4)新方法 发展。更具体地说，MIC将支持折扣服务来表征 微生物组和代谢组以及在风湿病发病机制中起重要作用的组织 包括粪便、痰、口腔、下呼吸道、肠粘膜和宫颈阴道粘膜。自.以来 数据分析方面的挑战往往会成为开展黏膜生产性研究的瓶颈 在免疫生物学方面，MIC还将支持免费和折扣服务以及综合组学数据培训 分析。MIC还将支持微生物：代谢物：免疫表型的实验验证 通过体外细胞刺激，通过提供服务、咨询和支持来扩大 厌氧和好氧细菌及基于密度梯度和流式细胞术的细菌分离和分离 感兴趣的免疫细胞。MIC将开发使用RNAseq的增强能力，以表征这两个 肠道组织中宿主和微生物基因的同时表达及其相关的多组集成数据 分析。MIC还将通过以下方式开发增强的代谢组数据注释能力 创建特定于组织的数据库，用于注释来自粪便和痰样本的非靶向LC/MS数据。 最后，MIC将投资开发用于体外分离感兴趣细胞群的新方法 对寄主的审问：微生物相互作用。通过整合具有不同专业知识的个人并提供 折扣服务、培训和支持，MIC将支持早期职业生涯和成熟的调查人员 在科罗拉多大学(CU)风湿病研究资源中心(RDRRC)，以及协助 新进入风湿病领域的研究人员，以提高整体研究的实施和生产力。

Summary: Resource Core 2: Mucosal Immunobiology Core (MIC) Compounding evidence exists for the role of microbiota and mucosal immunology in the pathogenesis of rheumatic diseases including rheumatoid arthritis (RA) and spondyloarthritis (SpA). The Mucosal Immunobiology Core (MIC) aims to provide consultation, discounted services, and development of enhanced capabilities to support the prediction and validation of mechanisms of host:microbe interactions in biospecimens of importance to rheumatic disease pathogenesis. The MIC will facilitate collaboration between individuals with expertise in microbiome, metabolome/lipidome, immunology, statistical data analysis, and rheumatic disease, which is essential for conducting this multidisciplinary research. Taken together, this core will support research into host:microbe interactions through 1) assembling individuals with diverse and complimentary expertise for promoting team science approaches focused on mucosal phenotypes and systemic consequences; 2) educating young investigators and those new to the field; 3) providing discounted services; and 4) novel methods development. More specifically, the MIC will support discounted services for the characterization of the microbiome and metabolome of biospecimens and tissues of importance in rheumatic disease pathogenesis, including feces, sputum, oral cavity, lower respiratory tract, intestinal mucosa, and cervicovaginal mucosa. Since challenges in data analysis can often be a bottleneck in conducting productive research in mucosal immunobiology, the MIC will also support free and discounted services and training in integrated ‘omic data analysis. The MIC will also support experimental validation of microbe:metabolite:immune phenotype relationships via in vitro cell stimulations, by providing services, consultation, and support in the expansion of anaerobic and aerobic bacteria and flow cytometry and density gradient based isolation of bacteria, and isolation of immune cells of interest. The MIC will develop enhanced capabilities in use of RNASeq to characterize both host and microbial gene expression simultaneously in intestinal tissue and associated multi’omic integrated data analyses. The MIC will also develop enhanced capabilities in annotation of metabolomic data through the creation of tissue specific databases for annotation of untargeted LC/MS data from feces and sputum samples. Finally, the MIC will invest in developing novel methods for isolating cell populations of interest for use in in vitro interrogations of host:microbe interactions. By integrating individuals with diverse expertise and providing discounted services, training and support, the MIC will support both early career and established investigators in the University of Colorado (CU) Rheumatic Disease Research Resource Center (RDRRC), as well as assist investigators new to the field of rheumatic disease to enhance overall research implementation and productivity.