Biomedical Core: Cellular and molecular mediators of fibrosis in the development of urinary tract dysfunction

项目摘要-生物医学研究核心 生物医学研究核心有三个主要目标：分析支持性小鼠的下尿功能 在U54奥布莱恩中心的三个项目中；参与并与调查人员合作，使这些 为泌尿外科研究界普遍提供的服务；以及完善和验证当前和新的 小鼠下尿路功能评价技术。奥布莱恩中心的首要目标是 良性泌尿系研究是为了确定导致下尿路功能障碍的机制 良性前列腺增生症(BPH)和下尿路症状(LUTS)。该中心的主要关注点是 BPH/LUTS进展过程中前列腺纤维化的发展及其与功能改变的关系 伴肝纤维化导致下尿路综合征。然而，患者并不会因为大脑结构的改变而寻求治疗。 前列腺，而不是需要治疗，以改善下尿路结石。因此，改善患者护理只能是 通过预防或治疗导致下尿路功能障碍(LUTS)的机械性事件来实现。它 因此，在BPH/LUTS小鼠模型中确定的机械性变化的影响是至关重要的 与下尿路功能改变相关。这些研究的结果对于确定 应考虑的管理策略，以促进临床应用。更低的整合 进入生物医学研究核心的尿功能测试允许在测试中进行最佳质量控制，改进 下尿路功能测试程序、新技术的开发和验证 老鼠。生物医学研究核心将提供严格的、可重复的下尿路功能测试 用于中心三个项目的MICE，以及K12 Kure学者在这一领域的支持需求。这个 生物医学研究核心的资源和专业知识也可供泌尿外科研究界使用 总体而言。生物医学研究核心与外部建立了多个富有成效的合作 调查人员，预计这些活动将在未来继续并扩大。与Dr合作 圣路易斯华盛顿大学的侯江辉已经演变成NIH R01的申请，比约林博士 是主要的PI，侯博士是获得高度竞争性优先级分数(第11个百分位数； R01DK118145，microRN29对膀胱结构和功能的调节；理事会会议定于5月举行 5、2019年)。生物医学研究中心还将致力于建立和验证下尿液的新方法 为了使这些技术在更大的研究社区中可用，我们将研究小鼠的肌束功能。因此， 生物医学研究核心不仅将提供测试服务，而且将成为改进和 评估小鼠下尿路功能的技术的可用性增加。

PROJECT SUMMARY – BIOMEDICAL RESEARCH CORE The Biomedical Research Core has three primary objectives: to analyze lower urinary function in mice in support of the three projects of the U54 O’Brien Center; to engage and collaborate with investigators to make these services generally available to the urology research community; and to refine and validate current and new techniques for evaluation of lower urinary tract function in mice. The overarching goal of the O’Brien Center for Benign Urology Research is to identify mechanisms that result in lower urinary tract dysfunction in patients with benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS). A primary focus of the Center is development of fibrosis in the prostate during the progression of BPH/LUTS and how altered function associated with fibrosis result in LUTS. However, patients do not pursue treatment for changes in the structure of their prostates but rather require treatment for improvement of LUTS. Thus, improved patient care can only be achieved by prevention or treatment of mechanistic events that lead to lower urinary tract dysfunction (LUTS). It is therefore vital that the effects of mechanistic changes identified in mouse models of BPH/LUTS be rigorously correlated with changes in lower urinary tract function. Results of these studies are crucial to identification of management strategies that should be considered for advancement to clinical application. Consolidation of lower urinary function testing into the Biomedical Research Core allows optimal quality control in testing, refinement of testing procedures, and development and validation of new techniques for testing lower urinary tract function in mice. The Biomedical Research Core will provide rigorous, reproducible testing of lower urinary tract function in mice for the three projects of the Center, as well as supporting needs of K12 KURe scholars in this area. The resources and expertise of the Biomedical Research Core are also available to the urology research community in general. The Biomedical Research Core has established multiple productive collaborations with external investigators, and it is anticipated that these will continue and expand in the future. Collaboration with Dr. Jianghui Hou at Washington University, St. Louis has evolved into an NIH R01 application for which Dr. Bjorling is the lead PI and Dr. Hou is the co-PI that received a highly competitive priority score (11th percentile; R01DK118145, Regulation of bladder structure and function by microRN29; Council meeting scheduled for May 5, 2019). The Biomedical Research Core will also work to establish and validate new methods of lower urinary tract function in mice and to make these techniques available to the larger research community. Thus, the Biomedical Research Core will not only provide testing service but will also act as a nidus for improvement and increased availability of techniques for evaluation of lower urinary tract function in mice.