Microbial Genomics Biomedical Core

微生物基因组学生物医学核心

• 批准号: 10700950
• 负责人: Anne-Catrin Uhlemann
• 金额: $ 23.29万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-24 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10700950
• 关键词: 16S ribosomal RNA sequencing; Adherence; Affect; Antimicrobial Resistance; Area; Bacteria; Bacterial Genes; Bacterial Infections; Benign; Bioinformatics; Biological; CRISPR/Cas technology; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Complement; Congenital Abnormality; Consultations; Disease; Disease model; Drug resistance; Education; Ensure; Environment; Event; Experimental Designs; Future; Genes; Genetic; Genetic Polymorphism; Genome; Genomic medicine; Genomics; Goals; Heme; Hi-C; Homeostasis; Human; Individual; Infection; Infection prevention; Investigation; Laboratories; Life Style; Medical center; Mentorship; Metabolic; Metabolism; Metagenomics; Methods; Microbe; Microbial Genetics; Mission; Modernization; Molecular Biology; Multi-Drug Resistance; Mus; Mutation; National Institute of Diabetes and Digestive and Kidney Diseases; New York City; Nucleic Acids; Organism; Participant; Pathogenesis; Pathogenicity; Pathology; Pathway interactions; Patients; Pattern; Persons; Phenotype; Plasmids; Play; Policies; Preparation; Public Health; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Resource Sharing; Resources; Ribosomal RNA; Role; Sample Size; Sampling; Services; Site; Students; Symptoms; System; Technology; Testing; Universities; Urinary tract infection; Urine; Urologic Diseases; Urology; Uropathogen; Urothelium; Vesico-Ureteral Reflux; base; biobank; cohort; comparative; comparative genomics; design; experience; experimental study; genome sequencing; human subject; improved; individual variation; instrument; interest; knockout gene; lectures; member; metagenome; microbial genomics; microbiome; microbiome analysis; mouse model; mutant; next generation; next generation sequencing; novel; outreach; pathogen; precision medicine; programs; prospective; response to injury; sample collection; symptomatology; tool; urinary; urologic; whole genome

MICROBIAL GENOMICS BIOMEDICAL CORE (MGBC): PROJECT SUMMARY/ABSTRACT The mission of the Columbia University O’Brien Urology Research Center is to identify genetic factors and congenital malformations, such as vesicoureteral reflux, that predispose individuals to urinary tract infections (UTI) and to explore host-bacterial metabolic processes, such as heme pathways, that enable or prevent infection. UTI represent one of the most common infections, affecting 150 million people per year worldwide. In support of the goals of the Columbia O’Brien Urology Research Center the Microbial Genomics and Biomedical Core (MGBC) will provide high quality services for biobanking, extended bacterial culture, microbial genomics (microbiome analyses, comparative genomics), microbial genetics, consultation for study design and outreach for novel investigations into the pathogenesis of urinary tract infections. The MGBC will enable Columbia O’Brien Center investigators to have access to specialized genomic technologies and coordinated expert consultations for improved rigor and reproducibility in genomics and precision medicine. In recognition of the increasing impact of antimicrobial resistance in urinary tract infections the MGBC has established a biorepository of multi-drug resistant UTI isolates. The services and biorepository are specifically designed to meet the needs of two of the Research Projects of the Columbia O’Brien Center and Opportunity Pool Awardees and will be available to the wider urology research community. The MGBC will accomplish these goals through the following aims: 1) Expand a biorepository of multi-drug resistant UTI isolate to multiple sites across New York City, establish a urine biorepository from clinically well-defined patients with different UTI symptoms and perform extended urine culture for organism identification; 2) Provide Microbiome and metagenome analyses and Hi-C/MetaPore-C sequencing; 3) Facilitate whole genome sequencing of dominant UTI pathogenic clones to elucidate bacterial determinants of UTI symptomatology; 4) Generate bacterial mutants in multi-drug resistant clinical UTI isolates leveraging a highly efficient CRISPR-Cas system; and 6) Provide consultations and support of study design for the Research Projects. Importantly, the MGBC will serve as a national resource for members of other George M. O'Brien Cooperative Research Centers and other NIDDK Urologic Research Programs. In close collaboration with the Administrative Core the MGBC will ensure adherence to all fiscal, administrative, and resource sharing policies. The MGBC will also contribute to the goals of the Educational Enrichment and Opportunity Pool Programs through lectures, student mentorship, and guidance on experimental design related to microbial genomics for Opportunity Pool awardees. Taken together, the Columbia O’Brien U54 MGBC will enable state- of-the-art characterization of microbes in UTIs, with a focus on evolving multi-drug resistance, by providing a complementary set of tools harnessing molecular biology, genetics, and ‘omics technologies.

微生物基因组学生物医学核心 (MGBC)：项目摘要/摘要 哥伦比亚大学奥布莱恩泌尿学研究中心的使命是识别遗传因素和 先天性畸形，例如膀胱输尿管反流，使个体容易患尿路感染 （尿路感染）并探索宿主细菌代谢过程，例如血红素途径，以促进或预防 感染。尿路感染是最常见的感染之一，每年影响全球 1.5 亿人。在 支持哥伦比亚奥布莱恩泌尿学研究中心微生物基因组学和生物医学的目标 Core（MGBC）将为生物样本库、扩展细菌培养、微生物基因组学提供高质量服务 （微生物组分析、比较基因组学）、微生物遗传学、研究设计和推广咨询 对尿路感染发病机制的新研究。 MGBC 将使 Columbia O’Brien 中心研究人员可以获得专门的基因组技术和协调的专家咨询 提高基因组学和精准医学的严谨性和可重复性。认识到日益增加的影响 MGBC 建立了一个多药生物库 耐药尿路感染分离株。服务和生物样本库是专门为满足其中两个的需求而设计的 哥伦比亚奥布莱恩中心和机会库获奖者的研究项目将提供给 更广泛的泌尿学研究界。 MGBC 将通过以下目标实现这些目标：1) 将多重耐药性尿路感染分离物的生物储存库扩大到纽约市的多个地点，建立一个 尿液生物储存库来自临床明确的具有不同 UTI 症状的患者，并进行扩展尿液分析 用于生物体鉴定的培养； 2) 提供微生物组和宏基因组分析以及Hi-C/MetaPore-C 测序； 3) 促进尿路感染显性致病克隆的全基因组测序，以阐明细菌 尿路感染症状的决定因素； 4) 在多重耐药临床尿路感染分离株中产生细菌突变体 利用高效的 CRISPR-Cas 系统； 6) 提供研究设计的咨询和支持 研究项目。重要的是，MGBC 将作为其他乔治城成员的国家资源 M. O'Brien 合作研究中心和其他 NIDDK 泌尿科研究项目。密切合作 MGBC 将通过行政核心确保遵守所有财政、行政和资源共享 政策。 MGBC 还将为教育丰富化和机会库的目标做出贡献 通过讲座、学生指导以及与微生物相关的实验设计指导来开展课程 机会池获奖者的基因组学。总而言之，哥伦比亚奥布莱恩 U54 MGBC 将使州- 对尿路感染中的微生物进行最先进的表征，重点是不断发展的多重耐药性，通过提供 利用分子生物学、遗传学和“组学技术”的互补工具集。