Microbial Genomics Biomedical Core

微生物基因组学生物医学核心

• 批准号: 10022313
• 负责人: Anne-Catrin Uhlemann
• 金额: $ 18.5万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-24 至 2021-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10022313
• 关键词: Acute; Adherence; Affect; Area; Bacterial Infections; Bioinformatics; CRISPR/Cas technology; Clinical; Communities; Congenital Abnormality; Consultations; Core Facility; Custom; Data Analyses; Disease; Disease model; Education; Ensure; Environment; Event; Experimental Designs; Gene Expression; Genes; Genetic; Genetic Polymorphism; Genome; Genomic medicine; Genomics; Genotype; Goals; Heme; Homeostasis; Human; Individual; Infection prevention; Investigation; Knock-in; Knock-out; Laboratories; Life Style; Medical center; Medicine; Mentorship; Metabolic; Metabolic Pathway; Metagenomics; Methods; Microbial Genetics; Microbiology; Mission; Modernization; Mus; Mutation; National Institute of Diabetes and Digestive and Kidney Diseases; Nucleic Acids; Oligonucleotide Primers; Organism; Participant; Pathogenesis; Pathogenicity; Pathology; Patients; Phenotype; Play; Policies; Property; RNA; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Resource Sharing; Resources; Ribosomal RNA; Role; Sample Size; Sampling; Services; Students; Symptoms; Technology; Testing; Time; Universities; Urinary tract infection; Urine; Urologic Diseases; Urology; Urothelium; Vesico-Ureteral Reflux; Virulence; Work; base; biobank; cohort; comparative genomics; design; experimental study; genome analysis; genome sequencing; genomic tools; human subject; improved; individual variation; instrumentation; interest; lectures; member; metagenome; metagenomic sequencing; microbial; microbial genomics; microbiome; microbiome analysis; mouse model; mutant; next generation; next generation sequencing; novel; pathogen; pathogen genomics; pathogenic microbe; precision medicine; programs; response; sample collection; transcriptome sequencing; urinary; urologic; whole genome

MICROBIAL GENOMICS BIOMEDICAL CORE (MGBC): PROJECT SUMMARY/ABSTRACT The mission of the Columbia University O’Brien Urology Research Center is to identify genetic factors and congenital malformations that predispose individuals to UTI, such as vesicoureteral reflux, to explore host- bacterial metabolic pathways, such as heme, that enable or prevent infection. To achieve this goal, the Microbial Genomics and Biomedical Core (MGBC) will provide high quality services for biobanking, extended bacterial culture, microbial genomics (microbiome analyses, comparative genomics, RNASeq), microbial genetics and consultation for study design. The MGBC will enable Columbia O’Brien Center investigators to have access to specialized genomic technologies, coordinated expert consultations and improved rigor and reproducibility in genomics and precision medicine. These services are specifically designed to meet the needs of the Research Projects of the Columbia O’Brien Center. Moreover, the MBMC will serve as a national resource for members of other George M. O'Brien Cooperative Research Centers and other NIDDK Urologic Research Programs. Additionally, the MGBC will work closely with the Administrative Core to ensure adherence to all fiscal, administrative, and resource sharing policies. The MGBC will also contribute to the goals of the Educational Enrichment and Opportunity Pool Programs through lectures, student mentorship, and guidance on experimental design related to microbial genomics for Opportunity Pool awardees. The MBMC will accomplish these goals through the following aims: 1) Establish a urine biorepository from clinically well-defined patients with different UTI symptoms; 2) Provide Microbiome and metagenome analyses; 3) Perform extended urine culture for organism identification; 4) Facilitate whole genome sequencing of dominant UTI pathogenic clones; 5) Determine gene expression by RNAseq and quantitative real-time PCR; 6) Generate bacterial mutants; and 7) Provide consultations and support of study design for the Research Projects.

微生物基因组学生物医学核心（MGBC）：项目总结/摘要 哥伦比亚大学奥布莱恩泌尿学研究中心的使命是确定遗传因素， 先天性畸形使个体易患UTI，如膀胱输尿管反流，以探索宿主- 细菌代谢途径，如血红素，使或防止感染。为了实现这一目标，微生物 基因组学和生物医学核心（MGBC）将为生物库，扩展细菌， 培养，微生物基因组学（微生物组分析，比较基因组学，RNASeq），微生物遗传学和 研究设计咨询。MGBC将使哥伦比亚奥布莱恩中心的调查人员能够访问 专门的基因组技术，协调专家咨询，提高严谨性和重现性， 基因组学和精准医疗。这些服务是专门为满足研究的需要而设计的。 哥伦比亚奥布莱恩中心的项目。此外，MBMC将作为国家资源， 另一个乔治M.奥布莱恩合作研究中心和其他NIDDK泌尿学研究项目。 此外，MGBC将与行政核心密切合作，以确保遵守所有财政， 行政和资源共享政策。MGBC还将为教育部的目标做出贡献。 丰富和机会池计划，通过讲座，学生辅导，并指导实验 与微生物基因组学相关的设计。MBMC将实现这些目标 通过以下目的：1）从临床明确定义的具有不同的尿液的患者中建立尿液生物储存库。 UTI症状; 2）提供微生物组和宏基因组分析; 3）进行扩展尿培养， 微生物鉴定; 4）促进显性UTI致病性克隆的全基因组测序; 5）确定 通过RNAseq和定量实时PCR的基因表达; 6）产生细菌突变体;和7）提供 为研究项目的研究设计提供咨询和支持。