SBIR TOPIC 107 AFFINITY REAGENTS FOR THE IMMUNE SYSTEM OF GOLDEN HAMSTER

叙利亚金黄地鼠是研究代谢性疾病和包括SARS-CoV-2在内的人类传染病的有价值的动物模型。然而，由于缺乏免疫试剂，仓鼠作为生物医学研究的小动物模型的有用性一直受到限制。在这份提案中，要约人建议为叙利亚金黄地鼠的八个与SARS-CoV-2感染和宿主免疫反应相关的免疫靶点开发亲和剂，并使用仓鼠免疫细胞表达的重组蛋白和内源性靶点来评估产生的抗体的亲和力和特异性。拟议工作的成功完成将产生一套全面的抗体组合，以促进对SARS-CoV-2的临床前研究。

Syrian golden hamsters are valuable animal models for studying metabolic disease and human infectious diseases including SARS-CoV-2. However, the usefulness of the hamster as a small animal model for biomedical research has been constrained by the lack of immunological reagents. In this proposal, the Offeror proposes to develop affinity reagents for eight immune targets of Syrian golden hamsters that are relevant to SARS-CoV-2 infections and host immune responses, and to evaluate affinity and specificity of the produced antibodies using recombinant proteins and endogenous targets expressed by immune cells from hamsters. Successful completion of the proposed work will result in a comprehensive portfolio of antibodies that facilitate pre-clinical studies on SARS-CoV-2.