SBIR TOPIC 107 AFFINITY REAGENTS FOR THE IMMUNE SYSTEM OF GOLDEN HAMSTER

SBIR 主题 107 金仓鼠免疫系统亲和试剂

• 批准号: 10688720
• 负责人: HONG QI
• 金额: $ 60万
• 依托单位: QOOLABS, INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2024-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10688720
• 关键词: 2019-nCoV; Affinity; Animal Model; Antibodies; Antigens; Binding; Biomedical Research; Cells; Communicable Diseases; Hamsters; Human; Immune; Immune Targeting; Immune response; Immune system; Immunologic Markers; Immunologics; Mesocricetus auratus; Metabolic Diseases; Monoclonal Antibodies; Oryctolagus cuniculus; Production; Reagent; Recombinant Proteins; SARS-CoV-2 infection; Small Business Innovation Research Grant; Specificity; Technology; Work; metabolic abnormality assessment; preclinical study

Syrian golden hamsters are valuable animal models for studying metabolic disease and human infectious diseases including SARS-CoV-2. However, the usefulness of the hamster as a small animal model for biomedical research has been constrained by the lack of immunological reagents. In this proposal, the Offeror proposes to develop affinity reagents for eight immune targets of Syrian golden hamsters that are relevant to SARS-CoV-2 infections and host immune responses, and to evaluate affinity and specificity of the produced antibodies using recombinant proteins and endogenous targets expressed by immune cells from hamsters. Successful completion of the proposed work will result in a comprehensive portfolio of antibodies that facilitate pre-clinical studies on SARS-CoV-2.

叙利亚金仓鼠是研究代谢性疾病和包括SARS-CoV-2在内的人类传染病的有价值的动物模型。然而，仓鼠作为生物医学研究的小动物模型的实用性受到缺乏免疫试剂的限制。本提案拟开发与SARS-CoV-2感染和宿主免疫应答相关的8个叙利亚金仓鼠免疫靶点的亲和试剂，并利用重组蛋白和仓鼠免疫细胞表达的内源性靶点评价制备的抗体的亲和性和特异性。拟议工作的成功完成将产生一个全面的抗体组合，促进SARS-CoV-2的临床前研究。