Accelerating Medicines Partnership-Autoimmune and Immunologic Disease Tissue Research Core

项目摘要 成功的加速RA/狼疮药物合作伙伴关系（AMP 1）计划专注于解构 通过单细胞转录组学技术研究疾病组织。AMP在自身免疫和免疫介导的 疾病（AMP AIM）有望扩大对细胞成分和相互作用的理解 在四种自身免疫性疾病靶组织中使用新的空间定向的单细胞分析模式。如在 AMP AIM将需要高质量的样品进行询问，标准化的方法来评估 所有受试者的样本质量和常见分子/表型生物标志物检测， 分子表型良好的队列应用新的尖端技术来重建疾病。 OMRF组织研究中心（TRC）的独特定位是成功部署集中式TRC 狼疮（SLE）、类风湿性关节炎（RA）、干燥病（SjD）和银屑病谱系疾病的所有疾病团队 （私营部门司）。凭借强大的现有基础设施、能力和领导力，OMRF TRC将提供 网络具有：1）标准化协议和手册，用于网络的所有部分，2）集中式 收集、运输、储存和传播的物流，3）集中的跨疾病QC和初始检测 持续收集所有样品，以便进行早期质量管理和鉴定 4）组织成像（基本组织学和初始高维）， 保留提供组织评分和质量控制的工作流程，以及初始多组学、空间信息数据集， 以及5）工业标准存储库和图像数据库系统。 OMRF TRC位于市中心，是物流管理的理想场所， AMP 1的存储库沿着网络知识，并具有丰富的经验， 在向AMP AIM过渡期间提供连续性。OMRF TRC 已经有了基础设施，以及强有力的领导所带来的声誉，继续作为 一个理想的诚实的经纪人为网络。OMRF TRC拥有最先进的设施， 在其相关核心内采用先进技术，以接收、存储、收集有意义的QC和初始数据集， 并将选定的样本分发给技术和分析核心（TAC）， 新兴技术建立在深入的临床和初始表征数据的基础上， 多维的强大数据集。 考虑到OMRF TRC在自身免疫性疾病生物库样本中的长期历史 研究，OMRF TRC准备为库存样品，有组织的物流， 初始多组学数据集，将设置网络，以成功重建我们的机制 这些新数据的理解和临床应用，以更好地治疗这些自身免疫性疾病。

Project Summary The successful Accelerating Medicines Partnership in RA/Lupus (AMP1) program focused on deconstructing disease tissues through single-cell transcriptomic technologies. AMP in Autoimmune and Immune-Mediated Diseases (AMP AIM) is poised to expand the understanding of the cellular components and interactions at play in four autoimmune disease target tissues with new spatially-oriented modalities of single-cell analyses. As in the AMP1, the AMP AIM will require high-quality samples for interrogation, standardized methods to assess sample quality and common molecular/phenotypic biomarker testing across all subjects for a clinically and molecularly well-phenotyped cohort to apply new cutting-edge technologies to re-construct disease. The OMRF tissue research core (TRC) is uniquely positioned to successfully deploy a centralized TRC across all Disease Teams for Lupus (SLE), RA, Sjogren’s Disease (SjD), and Psoriatic Spectrum Diseases (PSD). With a robust, existing infrastructure, capabilities, and leadership, the OMRF TRC will provide the Network with: 1) standardized protocols and manuals to be used across all parts of the Network, 2) centralized logistics for collection, transport, storage and dissemination, 3) centralized, trans-disease QC and initial testing of all samples in a continuous manner as samples are collected for early quality management and identification of samples of high importance, 4) tissue imaging (basic histology & initial high-dimensional) utilizing sample sparing workflows that provide scoring and QC of tissue, plus initial multi-omic, spatially-informed data sets, and 5) industry standard repository and image database systems. The OMRF TRC is centrally located, making it an ideal location for logistics management and, since it was the repository for AMP1 carries Network knowledge along with the extensive experience as repository for multiple other national consortiums, provides for continuity during transition to AMP AIM. The OMRF TRC already has infrastructure in place and the reputation that follows from strong leadership to continue to act as an ideal honest broker for the Network. The OMRF TRC has state-of-the-art facilities that are integrated with advanced technologies within its associated cores to receive, store, collect meaningful QC and initial data sets, and disseminate selected samples to technology and analytic cores (TAC) poised to provide data from emerging technologies to build upon the deep clinical and initial characterization data, to make extremely high- dimensional, robust datasets. Considering the longstanding history of the OMRF TRC in biobanking samples for autoimmune disease studies, the OMRF TRC is poised to a produce a solid foundation of banked samples, organized logistics, and initial multi-omic datasets that will setup the Network for success in reconstructing our mechanistic understanding and clinical application of these new data to better treat these autoimmune diseases.