Human Phenotyping Core

人类表型分析核心项目摘要 这个非常成功的人类表型核心集中于为中心研究人员提供访问 利用多参数、高内容的方法评估 风湿病研究的分子表型。该核心协调专门知识和核心能力 在转录组学、蛋白质组学、高含量细胞免疫表型、单细胞多组学和组织- 基于空间多组学。因此，它继续作为一种资源，在那里ORDRCC研究者可以产生， 共享和分析开发竞争力所需的多维分子表型数据 研究项目。除了让ORDRCC调查人员获得目前先进的技术外， 在OMRF成立，这个核心提供新开发的服务，提供单一的数据分析访问， 来自血液或解离组织的细胞多组学，以及基于空间感知组织的单细胞 接近。我们的平台使研究人员能够根据基因、 转录组、细胞和/或蛋白质组在特定细胞群体中的差异，以及在单个细胞中的差异， 单细胞水平10 X基因组学多组学方法，3'或5' scRNA-seq/high-plex CITE-seq + BCR/TCR库或snRNA-seq/snATAC-seq用于表观遗传分析。目前正在发展的方法， 在Chromium X平台上分离成单细胞的固定细胞或固定组织提供了额外的选择， 在某些情况下难以从现有的存档病理学样品中获得细胞群。空间技术 也取得了显著进展，包括多组学（转录物和蛋白质生物标志物）方法。 充分利用我们精心处理、组织良好、 临床表征样本;我们提供全面的多分析物分子表型的能力 简介为确定新的研究方向和产生新的想法提供了基础资源， 未来的合作项目。更新后的人类表型核心的目的是：AIM 1：辅助ORR CC 研究人员，JCI和学者导航项目开发，实验设计，资源 通过核心管理层和个人规划和执行转化和机械研究 咨询，目标2：提供使用分子单细胞和空间多组学 技术使用的技术人员在这些先进的方法，目的3：建立数据 用于质量控制、数据集生成和单像元和空间的高级建模的分析管道 多组学数据和患者分层的分子表型，AIM 4：评价、评估和实施 新兴技术和分析方法，以更准确地评估疾病患者的异质性 介绍和治疗反应。HPC评估新技术，与该机构合作， 确保新设备的安全或帮助调查人员了解新技术的优势和劣势。 HPC还为这些领域的JCI，学者，中心研究人员和实验室人员提供教育。

Human Phenotyping Core Project Summary This highly successful Human Phenotyping Core focuses on providing Center investigators with access to state-of-the-art technologies and methods for utilizing multi-parameter, high-content approaches to assess molecular phenotypes for rheumatic disease research. This Core coordinates expertise and core competencies in transcriptomics, proteomics, high-content cellular immunophenotypes, single-cell multiomics and tissue- based spatial multiomics. As such, it continues as a resource where ORDRCC Investigators can generate, share and analyze the multi-dimensional molecular phenotype data necessary for developing competitive research programs. Beyond giving ORDRCC investigators access to advanced technologies that are currently established at OMRF, this Core offers newly developed services providing access to data analytics in single- cell multi-omics from blood or dissociated tissue, as well as spatially aware tissue-based single-cell approaches. Our platforms enable investigators to define patient molecular profiles based on genetic, transcriptome, cellular, and/or proteomic differences in specific cell populations, as well as at the individual cell level with single cell 10X Genomics multiomic approaches, either 3’ or 5’ scRNA-seq/high-plex CITE-seq + BCR/TCR repertoire, or snRNA-seq/snATAC-seq for epigenetic analyses. Currently evolving approaches using fixed cells or fixed tissue dissociated to single cells on the Chromium X platform offer additional options for difficult to obtain cell populations from existing archival pathology samples in some cases. Spatial technologies have also advanced significantly and include multiomic (transcript and protein biomarkers) approaches. Capitalizing on the magnitude of information available through our carefully processed, well-organized, clinically characterized samples; our ability to provide comprehensive multi-analyte molecular phenotype profiles provide a foundational resource for identifying new research directions and generating new ideas for future collaborative projects. Aims of the updated Human Phenotyping Core are: AIM 1: Assist ORDRCC Investigators, JCIs and Scholars to navigate the stages of project development, experimental design, resource planning and execution of translational and mechanistic studies through Core Management and individual consultations, AIM 2: Provide access and training in use of molecular single-cell and spatial multiomic technologies using the technical personnel experienced in these advanced approaches, AIM 3: Establish data analytics pipelines for quality control, dataset generation and advanced modeling of single-cell and spatial multiomic data, and molecular phenotyping for patient stratification, AIM 4: Evaluate, assess and implement emerging technology and analytics approaches to more accurately assess patient heterogeneity in disease presentation and treatment responses. The HPC evaluates new technologies, working with the institution to secure new equipment or helping investigators understand the strengths and weaknesses of new technologies. The HPC also provides education for JCIs, Scholars, center investigators and lab personnel in these areas.