Human Phenotyping Core

人类表型核心

• 批准号: 10016171
• 负责人: Joel Marvin Guthridge
• 金额: $ 36.85万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-07 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10016171
• 关键词: Address; Autoantibodies; Autoimmune Diseases; Biological Assay; Biological Markers; Blood Cells; Caring; Cells; Characteristics; Clinical; Competence; Complex; Consultations; Data; Data Analyses; Data Set; Databases; Development; Diagnostic; Disease; Effectiveness; Ensure; Epigenetic Process; Equipment; Experimental Designs; Flow Cytometry; Foundations; Freezing; Funding; Future; Generations; Genetic; Genetic Polymorphism; Genomic DNA; Genomics; Genotype; Human; Human Herpesvirus 4; Human Resources; Immune; Immunologics; Immunophenotyping; Individual; Inflammatory; Investigation; Knowledge; Measures; Mediator of activation protein; Methodology; Methods; Molecular; Molecular Profiling; Oklahoma; Pathogenesis; Pathway interactions; Patients; Phenotype; Plasma; Population; Preparation; Process; Proteomics; Quality Control; Reproducibility; Research; Research Personnel; Resources; Rheumatism; Sampling; Serum; Services; Site; Specimen; Statistical Models; System; Technical Expertise; Technology; Therapeutic; Time; Transformed Cell Line; Variant; base; biobank; chemokine; clinical center; clinical phenotype; clinically relevant; computerized data processing; cytokine; data quality; design; experience; experimental study; human disease; immune function; molecular phenotype; novel; novel strategies; phenotypic data; precision medicine; prognostic tool; programs; response; single-cell RNA sequencing; success; transcriptome; transcriptomics

Human Phenotyping Core Project Summary The rapid development of new approaches to interrogate the immune and inflammatory systems comes with a growing demand for both technical and conceptual expertise in these methodologies. The Human Phenotyping Core was created to support these activities within the Oklahoma Rheumatic Disease Resources Cores Center (ORDRCC). To address the most broadly scientifically interesting and clinically important questions, our ORDRCC Investigators need access to cutting-edge approaches, such as scRNA-Seq/CITE- Seq, ChipCytometry, and CyTOF to perform incisive experiments and to generate high-quality, reproducible data. The Human Phenotyping Core will allow our investigators to ask novel questions regarding the molecular phenotypes of rheumatic diseases. In addition to providing ORDRCC investigators with high-content biomolecular phenotyping services, the Human Phenotyping Core in conjunction with the Clinical Characterization and Biorepository Core (CCBC) will provide access to datasets containing previously collected genetic, proteomic, and biomolecular phenotypes of clinical subjects whose samples are available from the CCBC. The tight integration of the Human Phenotyping Core, CCBC and our databases will enable investigators to efficiently phenotype samples and correlate molecular and cellular phenotypes with clinical characteristics, to select more homogeneous patients for hypothesis-driven investigation and help provide the foundational knowledge needed for precision medicine in rheumatic disease care. By consolidating a range of technologies required for the complete characterization of molecular or cellular phenotypes into a single unit, the Human Phenotyping Core (HPC) will streamline services to help ORDRCC investigators in the efficient planning, design, execution and data analysis required for their individual projects. The HPC will provide experience in performing research using clinical specimens, technical expertise in a wide range of molecular and bioassays, access to equipment and resources thus enabling cutting-edge approaches to address research questions, quality control assessment of the data collected and data processing suitable for higher end statistical modeling of phenotype/clinical associations. SPECIFIC AIM 1: Assist ORDRCC Investigators, JCIs and Scholars to navigate the stages of project development, experimental design, resource planning and execution of translational and mechanistic studies through Core Management and individual consultations. SPECIFIC AIM 2: Provide access to advanced molecular phenotyping technologies and experienced technical personnel for efficient execution of advanced experimental data plans. SPECIFIC AIM 3: Establish processes to ensure data quality control, processing of raw data, and preparation of data sets for more extensive analysis and statistical modeling. SPECIFIC AIM 4: Evaluate, assess and implement new cutting-edge technologies for molecular phenotyping.

人类表型分析核心项目摘要 询问免疫和炎症系统的新方法的快速发展 随着对这些方法的技术和概念专门知识的需求不断增长。人类 创建表型核心是为了支持俄克拉荷马州风湿病资源中的这些活动 核心中心（ORDCC）。为了解决最广泛的科学有趣和临床重要的 问题，我们的ORDRCC研究人员需要获得尖端的方法，如scRNA-Seq/CITE- Seq、ChipCytometry和CyTOF进行深入的实验，并生成高质量、可重复的 数据人类表型核心将允许我们的研究人员提出有关分子生物学的新问题。 风湿性疾病的表型除了为ORDRC研究人员提供高内容的 生物分子表型分析服务，人类表型分析核心与临床 表征和生物储存核心（CCBC）将提供对包含先前 收集的样本可用的临床受试者的遗传、蛋白质组和生物分子表型 来自CCBC。人类表型核心，CCBC和我们的数据库的紧密整合将使 研究人员有效地对样本进行表型分析，并将分子和细胞表型与临床 特征，选择更同质的患者进行假设驱动的研究，并帮助提供 风湿病护理中精准医学所需的基础知识。 通过整合一系列完整表征分子或 细胞表型整合到一个单元中，人类表型核心（HPC）将简化服务， 在有效的规划，设计，执行和数据分析所需的ORDRCC调查， 个别项目。HPC将提供使用临床标本进行研究的经验， 在广泛的分子和生物测定技术专长，获得设备和资源，从而 使尖端的方法来解决研究问题，数据的质量控制评估， 收集和数据处理适用于表型/临床关联的高端统计建模。 具体目标1：协助ORDRCC研究者、JCI和学者指导项目的各个阶段 开发、实验设计、资源规划和执行转化和机制研究 通过核心管理和个人咨询。 具体目标2：提供先进的分子表型技术和经验丰富的 技术人员的高效执行先进的实验数据计划。 具体目标3：建立确保数据质量控制、原始数据处理和准备的流程 用于更广泛的分析和统计建模。 具体目标4：评价，评估和实施新的尖端技术的分子表型。