Human Phenotyping Core

人类表型核心

• 批准号: 10016171
• 负责人: Joel Marvin Guthridge
• 金额: $ 36.85万
• 依托单位: OKLAHOMA MEDICAL RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-07 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10016171
• 关键词: Address; Autoantibodies; Autoimmune Diseases; Biological Assay; Biological Markers; Blood Cells; Caring; Cells; Characteristics; Clinical; Competence; Complex; Consultations; Data; Data Analyses; Data Set; Databases; Development; Diagnostic; Disease; Effectiveness; Ensure; Epigenetic Process; Equipment; Experimental Designs; Flow Cytometry; Foundations; Freezing; Funding; Future; Generations; Genetic; Genetic Polymorphism; Genomic DNA; Genomics; Genotype; Human; Human Herpesvirus 4; Human Resources; Immune; Immunologics; Immunophenotyping; Individual; Inflammatory; Investigation; Knowledge; Measures; Mediator of activation protein; Methodology; Methods; Molecular; Molecular Profiling; Oklahoma; Pathogenesis; Pathway interactions; Patients; Phenotype; Plasma; Population; Preparation; Process; Proteomics; Quality Control; Reproducibility; Research; Research Personnel; Resources; Rheumatism; Sampling; Serum; Services; Site; Specimen; Statistical Models; System; Technical Expertise; Technology; Therapeutic; Time; Transformed Cell Line; Variant; base; biobank; chemokine; clinical center; clinical phenotype; clinically relevant; computerized data processing; cytokine; data quality; design; experience; experimental study; human disease; immune function; molecular phenotype; novel; novel strategies; phenotypic data; precision medicine; prognostic tool; programs; response; single-cell RNA sequencing; success; transcriptome; transcriptomics

Human Phenotyping Core Project Summary The rapid development of new approaches to interrogate the immune and inflammatory systems comes with a growing demand for both technical and conceptual expertise in these methodologies. The Human Phenotyping Core was created to support these activities within the Oklahoma Rheumatic Disease Resources Cores Center (ORDRCC). To address the most broadly scientifically interesting and clinically important questions, our ORDRCC Investigators need access to cutting-edge approaches, such as scRNA-Seq/CITE- Seq, ChipCytometry, and CyTOF to perform incisive experiments and to generate high-quality, reproducible data. The Human Phenotyping Core will allow our investigators to ask novel questions regarding the molecular phenotypes of rheumatic diseases. In addition to providing ORDRCC investigators with high-content biomolecular phenotyping services, the Human Phenotyping Core in conjunction with the Clinical Characterization and Biorepository Core (CCBC) will provide access to datasets containing previously collected genetic, proteomic, and biomolecular phenotypes of clinical subjects whose samples are available from the CCBC. The tight integration of the Human Phenotyping Core, CCBC and our databases will enable investigators to efficiently phenotype samples and correlate molecular and cellular phenotypes with clinical characteristics, to select more homogeneous patients for hypothesis-driven investigation and help provide the foundational knowledge needed for precision medicine in rheumatic disease care. By consolidating a range of technologies required for the complete characterization of molecular or cellular phenotypes into a single unit, the Human Phenotyping Core (HPC) will streamline services to help ORDRCC investigators in the efficient planning, design, execution and data analysis required for their individual projects. The HPC will provide experience in performing research using clinical specimens, technical expertise in a wide range of molecular and bioassays, access to equipment and resources thus enabling cutting-edge approaches to address research questions, quality control assessment of the data collected and data processing suitable for higher end statistical modeling of phenotype/clinical associations. SPECIFIC AIM 1: Assist ORDRCC Investigators, JCIs and Scholars to navigate the stages of project development, experimental design, resource planning and execution of translational and mechanistic studies through Core Management and individual consultations. SPECIFIC AIM 2: Provide access to advanced molecular phenotyping technologies and experienced technical personnel for efficient execution of advanced experimental data plans. SPECIFIC AIM 3: Establish processes to ensure data quality control, processing of raw data, and preparation of data sets for more extensive analysis and statistical modeling. SPECIFIC AIM 4: Evaluate, assess and implement new cutting-edge technologies for molecular phenotyping.

人类表型核心项目总结 询问免疫和炎症系统的新方法的迅速发展 对这些方法的技术和概念专业知识的需求日益增长。《人类》 表型核心是为了支持俄克拉荷马州风湿病资源中的这些活动而创建的 岩心中心(ORDRCC)。以解决最具科学意义和临床重要性的 问题，我们的ORDRCC调查人员需要使用尖端方法，如scRNA-Seq/Cite- SEQ、芯片细胞术和CyTOF，以执行深入的实验并生成高质量、可重复性的 数据。人类表型核心将允许我们的研究人员提出关于分子的新问题 风湿性疾病的表型。除了为ORDRCC调查人员提供高内容 生物分子表型服务，人类表型核心与临床 特征和生物资源库核心(CCBC)将提供对包含以前 收集样本可用的临床受试者的遗传、蛋白质组和生物分子表型 来自CCBC。人类表型核心、CCBC和我们的数据库的紧密集成将使 研究人员高效地采集表型样本，并将分子和细胞表型与临床联系起来 特征，以选择更多同质性的患者进行假设驱动的研究，并帮助提供 风湿病护理中精准医学所需的基础知识。 通过整合完整表征分子或蛋白质的一系列技术 将细胞表型整合为一个单位，人类表型核心(HPC)将简化服务，以帮助 ORDRCC调查人员高效的规划、设计、执行和数据分析 个别项目。HPC将提供使用临床标本进行研究的经验， 在广泛的分子和生物分析方面的技术专长，从而获得设备和资源 采用前沿方法解决研究问题，对数据进行质量控制评估 适用于表型/临床关联的高端统计建模的收集和数据处理。 具体目标1：协助ORDRCC调查人员、JCI和学者浏览项目的各个阶段 翻译和机械研究的开发、实验设计、资源规划和执行 通过核心管理和个人协商。 具体目标2：提供先进的分子表型技术和经验 高效执行高级实验数据计划的技术人员。 具体目标3：建立流程以确保数据质量控制、原始数据处理和准备 用于更广泛的分析和统计建模的数据集。 具体目标4：评估、评估和实施新的前沿分子表型技术。