Micro-CT Imaging Core

Micro-CT 成像核心

• 批准号: 10691576
• 负责人: Xiaowei Sherry Liu
• 金额: $ 24.38万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10691576
• 关键词: 3-Dimensional; Acute; Aging; Animal Model; Animals; Biological Assay; Biological Process; Cartilage; Chronic; Clinical Research; Collaborations; Communities; Complement; Computer software; Consultations; Custom; Data; Data Analyses; Development; Disease; Doctor of Philosophy; Evaluation; Event; Faculty; Fibrocartilages; Floor; Foundations; Freezing; Funding; Genetic; Glycosaminoglycans; Gold; Health; Histologic; Histological Techniques; Histology; Home; Human; Image; Imaging Techniques; Immunohistochemistry; Intervertebral disc structure; Laboratories; Laboratory Animals; Location; Maintenance; Measurement; Mechanics; Methods; Minerals; Modality; Modeling; Morphology; Muscle; Musculoskeletal; Musculoskeletal Development; Musculoskeletal Diseases; Musculoskeletal Physiology; Musculoskeletal System; Paraffin; Pathologic; Pennsylvania; Peripheral; Philadelphia; Process; Research; Research Design; Research Personnel; Resolution; Resources; Roentgen Rays; Services; Skeleton; Stains; Structure; Techniques; Tendon structure; Time; Tissue Engineering; Tissue Model; Tissue Sample; Tissues; Training; University resources; X-Ray Computed Tomography; base; bone; bone imaging; calcification; clinical imaging; contrast enhanced; density; extracellular; human tissue; imaging approach; imaging modality; in vivo; injury and repair; innovation; insight; member; microCT; novel; open source; programs; protocol development; repaired; service delivery; soft tissue; three-dimensional visualization; tool

The mechanical and biological functions of musculoskeletal tissues are based on their structure, organization and composition. Maintenance of tissue integrity, acute changes with injury and repair, as well as progressive and chronic changes with aging and disease, can be evaluated and quantified on the sub-cellular, cellular, extracellular, and tissue level by histological techniques. Normal and pathological human tissues and animal models are widely used to study the cellular, extracellular, and tissue determinants of these processes. Thus, qualitative and analytical descriptions of key histological changes by standard and advanced staining techniques, and assessment and measurements of important histomorphological parameters in human tissue and animal models are at the foundation of examining critical questions in musculoskeletal health and disease. The overall objective of this Histology Core is to develop and apply a wide range of standard and innovative histological and histomorphometric approaches to evaluate musculoskeletal tissue structure and composition, and to provide training and funding for new projects and collaborations using these approaches. Due to their heterogeneous structures and compositions, musculoskeletal tissues present unique experimental and analytical challenges that require customized, sensitive and diverse histological assays. The PCMD Histology Core provides sophisticated expertise, and a suite of standard and novel tools and techniques, which are specially tailored for the complete range of musculoskeletal tissues, including intervertebral disc, bone, cartilage, fibrocartilage, tendon and muscle from large and small animal models, human tissue samples, and tissue-engineered constructs. Conventional techniques supported by the core include paraffin, frozen and plastic processing, embedding, sectioning and histochemical staining, as well as imaging and analysis, and more advanced and sophisticated techniques such as immunohistochemistry and histomorphometry. Additional new, cutting edge techniques will be added to the suite of core capabilities in this renewal. The Histology Core also provides educational programs for PCMD members and trainees through a range of mechanisms focusing on standard and advanced histological techniques, and their application to musculoskeletal research across a spectrum of healthy and disease conditions. With respect to service delivery, the core offers both full and self-service options, complemented by study design consultation and data interpretation to maximize the Core’s impact. A popular and fully subsidized protocol development and optimization service is offered to users to envision and jumpstart novel, application-specific histological techniques. Through this renewal, the Histology Core will continue to serve as a unique and indispensable resource for researchers at UPenn and in the wider community, catalyzing innovative and high impact musculoskeletal research, and stimulating collaborations between current and new PCMD members who may have not previously included histological approaches in their musculoskeletal research programs.

肌肉骨骼组织的机械和生物功能是基于它们的结构， 组织和构成。维持组织的完整性，损伤和修复的急性变化，以及 随着年龄和疾病的渐进性和慢性变化，可以在亚细胞上进行评估和量化， 通过组织学技术进行细胞、细胞外和组织水平的研究。正常和病理的人体组织和 动物模型被广泛用于研究这些过程的细胞、细胞外和组织决定因素。 因此，通过标准和高级染色对关键组织学变化进行定性和分析性描述 人体组织中重要组织形态参数的技术、评估和测量 动物模型是研究肌肉骨骼健康和疾病关键问题的基础。 该组织学核心的总体目标是开发和应用广泛的标准和 评估肌肉骨骼组织的创新组织学和组织形态计量学方法 结构和组成，并为新项目和协作提供培训和资金 使用这些方法。由于其结构和组成的异质性，肌肉骨骼组织 提出独特的实验和分析挑战，需要定制、敏感和多样化 组织学化验。PCMD组织学核心提供复杂的专业知识，以及一套标准和 专门为各种肌肉骨骼组织量身定做的新颖工具和技术， 包括大小动物的椎间盘、骨、软骨、纤维软骨、肌腱和肌肉 模型、人体组织样本和组织工程构建。支持的传统技术 岩心包括石蜡、冷冻和塑料加工、包埋、切片和组织化学染色 如成像和分析，以及更先进和复杂的技术，如免疫组织化学 和组织形态计量学。中的核心功能套件中将添加其他新的尖端技术 这次更新。组织学核心还通过以下方式为PCMD成员和受训人员提供教育计划 一系列侧重于标准和高级组织学技术的机制及其在 肌肉骨骼研究涵盖了一系列健康和疾病状况。关于服务 交付，核心提供全面和自助服务选项，并辅之以研究设计咨询和数据 解释以最大限度地提高核心的影响。广受欢迎且得到全额补贴的协议开发和 为用户提供优化服务，以预见和启动新的、特定于应用的组织学 技巧。通过这次更新，组织学核心将继续作为一个独特和不可或缺的 为宾夕法尼亚大学和更广泛的社区的研究人员提供的资源，催化创新和高影响力 肌肉骨骼研究，并促进PCMD现有成员和新成员之间的合作，这些成员可能 以前没有将组织学方法纳入他们的肌肉骨骼研究计划。