Activation of the NOD1 and NOD2 signaling pathways
NOD1 和 NOD2 信号通路的激活
基本信息
- 批准号:10041261
- 负责人:
- 金额:$ 18.81万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-06-01 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:ATF6 geneActinsAutophagocytosisBacterial InfectionsBindingBiological ProcessBiologyCalciumCell membraneCell physiologyCellsCytoskeletonDataEndoplasmic ReticulumHomeostasisImmune responseInfectionInflammationInterleukin-6KnowledgeMediatingMembraneMitochondriaModelingMolecularParasitesPatternPattern recognition receptorPeptidoglycanProductionProtein InhibitionProteinsRegulationRoleSignal PathwaySignal TransductionSignal Transduction PathwayTRAF2 geneTestingThapsigarginTranscriptional ActivationTranslationsTunicamycinVirusVirus DiseasesWorkendoplasmic reticulum stressexperimental studyinnovationmutantp21-activated kinase 1pathogenprotein misfoldingreceptorrecruitrelease of sequestered calcium ion into cytoplasmresponserho GTP-Binding Proteinstranscription factor
项目摘要
PROJECT SUMMARY
NOD1 and NOD2 are pattern recognition receptors that sense fragments of bacterial peptidoglycans,
and are able to detect perturbations in cellular processes such as the modulation of the actin cytoskeleton and
disturbance in endoplasmic reticulum (ER) homeostasis. Under different stressful conditions, such as bacterial
and viral infections, protein misfolding and perturbations in calcium homeostasis, the ER is unable to maintain
homeostasis and activates the unfolded protein response (UPR). Upon ER stress three transmembrane
receptors, IRE1, PERK and ATF6 are activated and regulate biological processes such as inhibition of protein
translation, autophagy, and inflammation to reestablish cellular homeostasis. NOD1 and NOD2 have been
implicated in ER stress-induced inflammation, by acting downstream in the UPR to induce NF-B activation
and IL-6 production. The exact mechanism how NOD1 and NOD2 can sense ER stress is currently unknown.
NOD1 and NOD2 can also sense the activation of small Rho GTPases such as Rac1. Rac1 activation leads to
membrane ruffling as well as activation of the transcription factor NF-B. We and others have shown that
NOD1 and NOD2 interact with Rac1 at the cell membrane. The underlying mechanism of Rac1-mediated
NOD1 and NOD2 activation is currently unknown. In the application we propose to study the mechanisms of
peptidoglycan-independent activation of NOD1 and NOD2 by modulation of the actin cytoskeleton and
thapsigargin-induced ER stress. Our central hypothesis is that NOD1 and NOD2 can detect cellular
perturbations independent of peptidoglycan recognition. We will test key aspects of our hypothesis using the
logical and innovative approach outlined in the following specific aims.
Specific Aim 1. Determine the role of NOD1 and NOD2 in sensing ER stress. We will determine the
contribution of calcium flux from the ER to the mitochondria in ER stress induced NOD1 and NOD2 activation.
We will test our hypothesis that mitochondria damaged by ER stress release damage-associated molecular
patterns (DAMPs) that activate NOD1 and NOD2.
Specific Aim 2. Perturbations in cellular processes determines NOD1 and NOD2 localization. We
will investigate the cellular localization of NOD1 and NOD2 in cells treated with thapsigargin to induce ER
stress and in cells that express active Rac1 or Rac1 mutant forms that either induce cytoskeletal remodeling or
NF-B activation. Characterizing and understanding the mechanisms of peptidoglycan-independent NOD1 and
NOD2 activation provides a plausible explanation for the observation that viruses and parasites trigger NOD1
and NOD2 signaling. These findings are innovative new concepts and would markedly influence the current
concepts of NOD1 and NOD2 biology.
项目摘要
NOD 1和NOD 2是模式识别受体,其感测细菌肽聚糖的片段,
并且能够检测细胞过程中的扰动,例如肌动蛋白细胞骨架的调节,
内质网(ER)稳态紊乱。在不同的压力条件下,如细菌
以及病毒感染、蛋白质错误折叠和钙稳态的扰动,
体内平衡和激活未折叠蛋白反应(UPR)。ER应激后,三个跨膜
受体IRE 1 β、PERK和ATF 6被激活并调节生物过程,例如蛋白质表达的抑制,
翻译、自噬和炎症以重建细胞内稳态。NOD 1和NOD 2已经被
参与ER应激诱导的炎症,通过作用于UPR下游诱导NF-κ B B活化
和IL-6的产生。NOD 1和NOD 2如何感知内质网应激的确切机制目前尚不清楚。
NOD 1和NOD 2也可以感知小Rho GTP酶如Rac 1的激活。Rac 1激活导致
膜皱褶以及转录因子NF-κ B B的激活。我们和其他人已经证明,
NOD 1和NOD 2在细胞膜上与Rac 1相互作用。Rac 1介导的潜在机制
NOD 1和NOD 2的激活目前尚不清楚。在本申请中,我们提出研究
通过调节肌动蛋白细胞骨架,非肽聚糖依赖性激活NOD 1和NOD 2,
毒胡萝卜素诱导的内质网应激。我们的中心假设是NOD 1和NOD 2可以检测细胞
不依赖于肽聚糖识别的扰动。我们将测试我们的假设的关键方面使用
在以下具体目标中概述了逻辑和创新方法。
具体目标1。确定NOD 1和NOD 2在感受ER应力中的作用。康贝特人将以
在内质网应激诱导的NOD 1和NOD 2激活中,从内质网到线粒体的钙流的贡献。
我们将验证我们的假设,即内质网应激损伤的线粒体释放损伤相关分子,
激活NOD 1和NOD 2的DAMP。
具体目标2。细胞过程中的扰动决定了NOD 1和NOD 2的定位。我们
将研究NOD 1和NOD 2在用毒胡萝卜素处理以诱导ER的细胞中的细胞定位
应激和表达活性Rac 1或Rac 1突变体形式的细胞中,所述活性Rac 1或Rac 1突变体形式诱导细胞骨架重塑或
NF-κ B B活化。表征和理解肽聚糖非依赖性NOD 1和
NOD 2激活为病毒和寄生虫触发NOD 1的观察提供了合理的解释
NOD 2信号这些发现是创新的新概念,将显著影响当前的
NOD 1和NOD 2生物学的概念。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Arina Marijke Keestra-Gounder的其他文献
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{{ truncateString('Arina Marijke Keestra-Gounder', 18)}}的其他基金
The impact of ER stress on Salmonella Typhimurium infections
内质网应激对鼠伤寒沙门氏菌感染的影响
- 批准号:
10708073 - 财政年份:2022
- 资助金额:
$ 18.81万 - 项目类别:
The impact of ER stress on Salmonella Typhimurium infections
内质网应激对鼠伤寒沙门氏菌感染的影响
- 批准号:
10565316 - 财政年份:2022
- 资助金额:
$ 18.81万 - 项目类别:
NOD2 promotes coxsackievirus entry and pathogenesis
NOD2促进柯萨奇病毒进入和发病机制
- 批准号:
10407068 - 财政年份:2021
- 资助金额:
$ 18.81万 - 项目类别:
NOD2 promotes coxsackievirus entry and pathogenesis
NOD2促进柯萨奇病毒进入和发病机制
- 批准号:
10285845 - 财政年份:2021
- 资助金额:
$ 18.81万 - 项目类别:
Activation of the NOD1 and NOD2 signaling pathways
NOD1 和 NOD2 信号通路的激活
- 批准号:
10172844 - 财政年份:2020
- 资助金额:
$ 18.81万 - 项目类别:
Targeting ER Stress in Inflammatory Bowel Disease
针对炎症性肠病的内质网应激
- 批准号:
9019324 - 财政年份:2016
- 资助金额:
$ 18.81万 - 项目类别:
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