Tumor Model and Biospecimen Repository Core

肿瘤模型和生物样本库核心

• 批准号: 10707167
• 负责人: ADEL K. EL-NAGGAR
• 金额: $ 6.72万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707167
• 关键词: Animal Model; Biological Markers; Biological Specimen Banks; Blood; Cancer cell line; Cell Line; Cell Maintenance; Clinical; Collaborations; Collection; Cytology; Database Management Systems; Databases; Development; Diagnosis; Drug or chemical Tissue Distribution; Ensure; Evaluation; Fine needle aspiration biopsy; Fostering; Genomics; Guidelines; Harvest; Head and Neck Cancer; Head and Neck Squamous Cell Carcinoma; Health Insurance Portability and Accountability Act; Human Resources; Immunohistochemistry; Individual; Laboratories; Lead; Leadership; Link; Malignant - descriptor; Medicine; Modeling; Mus; Nucleic Acids; Pathologic; Pathology; Patients; Preparation; Process; Research; Research Personnel; Resistance; Resources; Sampling; Secure; Services; Specimen; Techniques; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; United States National Institutes of Health; University of Texas M D Anderson Cancer Center; Western Blotting; college; cytokine; immunosuppressed; innovation; patient derived xenograft model; peripheral blood; programs; repository; tumor

Tumor Model and Biospecimen Repository Core (Core C) SUMMARY Tumor Model and Biospecimen Repository Core (Core C) led by Dr. Adel K. El-Naggar (Core Leader) and Dr. Jeffrey Myers (Core Co-Leader), will provide H-CARR investigators with a centralized facility that ensures the systematic collection, processing, storage, and distribution of peripheral blood, surrogate and specialized specimens, and samples of head and neck squamous cell carcinoma (HNSCC) from patients diagnosed, treated, and followed at The University of Texas MD Anderson Cancer Center (MD Anderson) at Baylor College of Medicine (BCM). Core C also provides expert preparation and distribution of high-quality samples and ancillary techniques to optimize utilization of patients’ materials by H-CARR investigators. Procurement and distribution of the samples to individual investigators will follow the guidelines established by the H-CARR Center Executive Leadership Committee and in compliance with HIPAA. Core C leadership will provide detailed pathologic evaluation of all tumor samples to investigators through the secure pathology database system linked to the H- CARR, database, and server. Core C will perform specialized services, including tissue microarray (TMA) construction, qualitative and quantitative biomarker analyses both in tissue [e.g., immunohistochemistry (IHC), Western blotting] and non-tissue (e.g., blood-based cytokine profiles) specimens, nucleic acid extractions, and development and maintenance of cell lines. Core C will also provide well-characterized, genomically authenticated head and neck cancer cell lines, well-characterized patient-derived xenograft (PDX) models, and heterotopic and orthotopic animal models in immunosuppressed or syngeneic mice and autochthonous tumor models of HNSCC to investigators. The leadership and personnel of Core C and its centralized organization will ensure the highest quality tissue samples and related analyses to all H-CARR investigators and to collaborating researchers participating in other NIH/NCI-sponsored programs.

肿瘤模型和生物质谱库核心(核心C)综述 由Adel K.El-Naggar博士(核心领导)和Dr. Jeffrey Myers(核心联席领导)将为H-CARR调查人员提供中央设施，以确保 系统地采集、加工、储存和分配外周血、代用品和专用血 样本，以及头颈部鳞状细胞癌(HNSCC)患者的样本， 之后在德克萨斯大学贝勒学院的MD安德森癌症中心(MD Anderson) 医学(BCM)。核心C还提供专家准备和分发高质量的样品和辅助 H-CARR研究人员优化患者材料利用的技术。采购和分销 将样本交给个人调查人员将遵循H-CARR中心执行人员制定的指导方针 领导委员会，并遵守HIPAA。核心C领导层将提供详细的病理 通过安全的病理数据库系统向调查人员评估所有肿瘤样本 CARR、数据库和服务器。核心C将提供专门的服务，包括组织微阵列(TMA) 组织中的构建、定性和定量生物标记物分析[例如，免疫组织化学(IHC)， Western blotting]和非组织(例如，基于血液的细胞因子图谱)样本、核酸提取和 细胞系的发展和维护。核心C还将提供具有良好特性的、基因组学上的 经过鉴定的头颈部癌细胞系，患者来源的异种移植(PDX)模型，以及 免疫抑制或同基因小鼠的异位和原位动物模型及自体肿瘤 向调查人员提供HNSCC模型。核心C及其集中组织的领导和人员将 确保向所有H-CARR研究人员和合作人员提供最高质量的组织样本和相关分析 参与NIH/NCI赞助的其他项目的研究人员。