Molecular, Cellular and Genetic Core Component

分子、细胞和遗传核心成分

• 批准号: 7612852
• 负责人: PING-YEE LAW
• 金额: $ 21.69万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7612852
• 关键词: Animals; Area; Base Sequence; Breeding; Budgets; Cell Line; Cell model; Cellular biology; Charge; Commercial Sources; Communities; Consult; Cost Savings; Development; Equipment and supply inventories; Facility Construction Funding Category; Funding; Genetic; In Vitro; Individual; Libraries; Minnesota; Modeling; Molecular; Mus; Mutant Strains Mice; Neurons; Oligonucleotide Synthesis Facility; Oligonucleotides; Opioid Receptor; Purpose; RNA Interference; Reagent; Recombinant Proteins; Research; Research Personnel; Resources; Services; Small Interfering RNA; Thinking; Universities; Vendor; Viral Vector; bacterial vector; base; cost; expression vector; in vivo; member; mouse model; mutant mouse model; programs; reagent standardization; success; vector

The Molecular Cellular and Genetic Core (MCGC) is being reviving in the Center for the purpose of providing expertise in the areas of molecular and cell biology and assisting in the construction of various vectors used in the proposed studies within the Center. The MCGC will provide all the scientific components the primary cultures from multant mice needed for their studies. The MCGC also will maintain lines of mutant mice that are used by multiple components and are highly requested by the scientific community. By centralizing all these functions in MCGC will allow not only substantial cost saving but also the standardization of the reagents, vectors, cultures and animals in all the projects within the Center. Therefore, the specific aims of MCGC are: 1) To establish banks of reagents and cell models to be used by Center's investigators. The revived MCGC will resume the duties in maintaining the inventories of vectors, bacteria strains, libraries and cell lines generated by the Center investigators from Administrative Core. MCGC will be responsible for sending out any request for the reagents and cell models, and expand the inventories to include viral and vector constructs that are useful for the in vitro primary culture and in vivo animal studies. 2) To provide expertise in the areas of molecular and cell biology to Center's investigators. The MCGC will consult on the construction of the various mammalian expression vectors, or targeting vectors for the development of genetic altered animals. In addition, MCGC will assist in constructing the siRNA vector and viral vectors for delivery the siRNA ortransgenes to be used by the Center's investigators. 3) To maintain a bank of mutant mouse models for the Center's investigators. In order to eliminate the drain in the individual investigator's resources, MCGC will maintain breeding colonies of mouse models so as to fulfill the requests by the Center's investigators and external investigators. 4) To provide primary cultures from wild type and mutant mice for the studies of opioid receptor action. The use of primary cultures as models is a common theme among all 5 scientific components of this proposed Center. Especially, the use of primary neurons from mutant mouse models is critical for establishing the validating of the cell models' observations. Thus, the MCGC will initiate the program of supplying primary cultures to Center's investigators. This program not only could utilize the primary cultures obtained from mutant mice more efficiently, it could also standardize the quality of the primary among all investigators within the Center.

分子细胞和遗传核心(MCGC)正在该中心复兴，目的是提供 在分子和细胞生物学领域的专业知识，并协助构建所使用的各种载体 在该中心内的拟议研究中。MCGC将提供所有主要的科学组件 他们的研究所需的多种鼠的培养。MCGC还将维持突变小鼠品系， 被多个部件使用，并受到科学界的高度要求。通过集中所有 MCGC中的这些功能不仅可以节省大量成本，还可以使 中心内所有项目中的试剂、病媒、培养物和动物。因此，该计划的具体目标 MCGC是：1)建立供中心研究人员使用的试剂库和细胞模型。 恢复的MCGC将恢复维护载体、细菌菌株、文库的库存 以及由来自管理核心的中心调查人员产生的细胞系。MCGC将负责 发出任何关于试剂和细胞模型的请求，并扩大库存以包括病毒和 对体外原代培养和体内动物研究有用的载体构建。2)提供 向中心的研究人员提供分子和细胞生物学领域的专业知识。MCGC将咨询 关于各种哺乳动物表达载体的构建，或用于开发的靶向载体 基因改变的动物。此外，MCGC还将协助构建siRNA载体和病毒载体 交付siRNA或转基因，以供中心的调查人员使用。3)维持一批变种人 为该中心的研究人员提供小鼠模型。为了消除个人的排泄物 调查人员的资源，MCGC将维护小鼠模型的繁育群体，以满足要求 中心的调查人员和外部调查人员。4)提供野生型的原代培养 和突变小鼠用于研究阿片受体的作用。使用原生文化作为模型是一种 拟建中心的所有5个科学组成部分的共同主题。尤其是对初级的使用 来自突变小鼠模型的神经元对于验证细胞模型的观察结果至关重要。 因此，MCGC将启动向中心的研究人员提供原代培养物的计划。这 该计划不仅可以更有效地利用从突变小鼠那里获得的原代培养物，还可以 在中心内的所有调查人员中标准化初级调查的质量。