Molecular, Cellular and Genetic Core Component

分子、细胞和遗传核心成分

• 批准号: 7612852
• 负责人: PING-YEE LAW
• 金额: $ 21.69万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-30 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7612852
• 关键词: Animals; Area; Base Sequence; Breeding; Budgets; Cell Line; Cell model; Cellular biology; Charge; Commercial Sources; Communities; Consult; Cost Savings; Development; Equipment and supply inventories; Facility Construction Funding Category; Funding; Genetic; In Vitro; Individual; Libraries; Minnesota; Modeling; Molecular; Mus; Mutant Strains Mice; Neurons; Oligonucleotide Synthesis Facility; Oligonucleotides; Opioid Receptor; Purpose; RNA Interference; Reagent; Recombinant Proteins; Research; Research Personnel; Resources; Services; Small Interfering RNA; Thinking; Universities; Vendor; Viral Vector; bacterial vector; base; cost; expression vector; in vivo; member; mouse model; mutant mouse model; programs; reagent standardization; success; vector

The Molecular Cellular and Genetic Core (MCGC) is being reviving in the Center for the purpose of providing expertise in the areas of molecular and cell biology and assisting in the construction of various vectors used in the proposed studies within the Center. The MCGC will provide all the scientific components the primary cultures from multant mice needed for their studies. The MCGC also will maintain lines of mutant mice that are used by multiple components and are highly requested by the scientific community. By centralizing all these functions in MCGC will allow not only substantial cost saving but also the standardization of the reagents, vectors, cultures and animals in all the projects within the Center. Therefore, the specific aims of MCGC are: 1) To establish banks of reagents and cell models to be used by Center's investigators. The revived MCGC will resume the duties in maintaining the inventories of vectors, bacteria strains, libraries and cell lines generated by the Center investigators from Administrative Core. MCGC will be responsible for sending out any request for the reagents and cell models, and expand the inventories to include viral and vector constructs that are useful for the in vitro primary culture and in vivo animal studies. 2) To provide expertise in the areas of molecular and cell biology to Center's investigators. The MCGC will consult on the construction of the various mammalian expression vectors, or targeting vectors for the development of genetic altered animals. In addition, MCGC will assist in constructing the siRNA vector and viral vectors for delivery the siRNA ortransgenes to be used by the Center's investigators. 3) To maintain a bank of mutant mouse models for the Center's investigators. In order to eliminate the drain in the individual investigator's resources, MCGC will maintain breeding colonies of mouse models so as to fulfill the requests by the Center's investigators and external investigators. 4) To provide primary cultures from wild type and mutant mice for the studies of opioid receptor action. The use of primary cultures as models is a common theme among all 5 scientific components of this proposed Center. Especially, the use of primary neurons from mutant mouse models is critical for establishing the validating of the cell models' observations. Thus, the MCGC will initiate the program of supplying primary cultures to Center's investigators. This program not only could utilize the primary cultures obtained from mutant mice more efficiently, it could also standardize the quality of the primary among all investigators within the Center.

分子细胞和遗传核心（MCGC）正在中心复兴，目的是提供 分子和细胞生物学领域的专业知识，并协助构建所用的各种载体 在中心内进行研究。MCGC将提供所有的科学组成部分， 他们的研究所需的多突变体小鼠的培养物。MCGC还将保持突变小鼠的品系， 由多个组件使用，并受到科学界的高度要求。通过集中所有 MCGC中的这些功能不仅可以节省大量的成本，而且还可以使 试剂、载体、培养物和动物。因此， MCGC的主要任务是：1）建立供中心研究者使用的试剂库和细胞模型库。 恢复运作后的MCGC将恢复管理病媒、细菌菌株和文库的职责。 和由中心研究人员从行政核心产生的细胞系。MCGC将负责 发出对试剂和细胞模型的任何要求，并扩大库存，包括病毒和 可用于体外原代培养和体内动物研究的载体构建体。2)提供 在分子和细胞生物学领域的专业知识，以中心的研究人员。MCGC将咨询 在构建各种哺乳动物表达载体或用于开发 基因改变的动物此外，MCGC将协助构建siRNA载体和病毒载体， 递送siRNA或转基因以供中心的研究人员使用。3)维持突变体库 为该中心的研究人员提供小鼠模型。为了消除个人的流失， 研究者的资源，MCGC将维持小鼠模型的繁殖群体，以满足要求 中心的调查人员和外部调查人员。4)提供野生型原代培养物 和突变小鼠用于阿片受体作用的研究。使用原代培养物作为模型， 该中心所有5个科学组成部分的共同主题。特别是使用primary 来自突变小鼠模型的神经元对于建立细胞模型观察的验证是至关重要的。 因此，MCGC将启动向中心研究者提供原代培养物的计划。这 程序不仅可以更有效地利用从突变小鼠获得的原代培养物， 规范中心内所有研究者的主要研究质量。