The Protein Complex Map of the Drosophila Proteome

描述(申请人提供)：大多数细胞过程是由多蛋白质复合体完成的。这种复合体的鉴定和组成对于理解表达的蛋白质集合(蛋白质组)是如何组织成功能单位至关重要。该提案的目标是基于对与单独标记的蛋白质相关的纯化蛋白质复合体的分离和质谱分析，建立完整的果蝇黑腹果蝇蛋白质组相互作用图。这将决定第一个超越二元蛋白质相互作用水平的完整的后生动物蛋白质组图谱。我们计划使用的实验系统提供了一种在遗传和基因组水平上已被广泛研究的有机体，具有作为脊椎动物生物学模型的既定有效性，并提供了通过复杂的基因操作来确定细胞功能的可能性。

DESCRIPTION (provided by applicant): Most cellular processes are carried out by multi-protein complexes. The identification and composition of such complexes is crucial for understanding how the ensemble of expressed proteins (The Proteome) is organized into functional units. The goal of the proposal is to establish a complete protein interaction map of the Drosophila melanogaster proteome based on the isolation and Mass spectrometric analysis of purified protein complexes associated with individually tagged proteins. This will determine the first complete metazoan proteome map beyond the level of binary protein interactions. The experimental system we propose to use offers an organism which has been extensively studied at a genetic and genomic level, has an established validity as a model for vertebrate biology, and offers the possibility of sophisticated genetic manipulations to determine cellular function.