The Protein Complex Map of the Drosophila Proteome

描述（由申请人提供）：大多数细胞过程由多蛋白复合物进行。这种复合物的鉴定和组成对于理解表达的蛋白质（蛋白质组）的集合如何组织成功能单元至关重要。该提案的目标是建立一个完整的蛋白质相互作用的地图的果蝇蛋白质组的基础上分离和纯化的蛋白质复合物与单独标记的蛋白质的质谱分析。这将决定第一个完整的后生动物蛋白质组图谱超越二元蛋白质相互作用的水平。我们建议使用的实验系统提供了一种生物体，它已被广泛研究在遗传和基因组水平上，具有作为脊椎动物生物学模型的既定有效性，并提供了复杂的遗传操作，以确定细胞功能的可能性。

DESCRIPTION (provided by applicant): Most cellular processes are carried out by multi-protein complexes. The identification and composition of such complexes is crucial for understanding how the ensemble of expressed proteins (The Proteome) is organized into functional units. The goal of the proposal is to establish a complete protein interaction map of the Drosophila melanogaster proteome based on the isolation and Mass spectrometric analysis of purified protein complexes associated with individually tagged proteins. This will determine the first complete metazoan proteome map beyond the level of binary protein interactions. The experimental system we propose to use offers an organism which has been extensively studied at a genetic and genomic level, has an established validity as a model for vertebrate biology, and offers the possibility of sophisticated genetic manipulations to determine cellular function.