EBNA3C and tumor supressors

eb病毒（EBV）是一种普遍存在的人类病毒，感染超过95%的成年人。感染eb病毒通常导致传染性单核细胞增多症的年轻人，并导致终身感染。EBV还与许多人类癌症有关，包括伯基特淋巴瘤、鼻咽癌、霍奇金淋巴瘤、成人T细胞淋巴瘤和免疫功能低下移植（PTLD）和HIV阳性艾滋病患者（ALD）的淋巴细胞增生性疾病。在体外，EBV可以转化人原代B细胞，使原代细胞永久增殖为转化的淋巴母细胞样细胞系（LCLs）。这些新生感染的B淋巴细胞表达一组精选的潜伏基因，其中之一是eb - Barr核抗原（EBNA） 3C，这是B细胞转化所必需的。本应用程序的总体目标是表征EBNA3C与众所周知的肿瘤抑制因子p53的关系，并确定由EBNA3C相互作用诱导的p53调控所需的翻译后修饰。这些研究首次直接检测了p53与EBV核心抗原EBNA3C的协同作用。

Epstein‐Barr virus (EBV) is a ubiquitous human virus infecting greater than 95% of the adult population. Infection with EBV typically leads to infectious mononucleosis in young adults and results in a lifelong infection. EBV is also associated with a number of human cancers which include Burkitt’s lymphoma, nasopharyngeal carcinoma, Hodgkin’s lymphoma, adult T‐cell lymphomas and lymphoproliferative diseases in immunocompromised transplant (PTLD) and HIV positive AIDS patients (ALD). In vitro, EBV can transform human primary B‐cells in vitro which results in perpetual proliferation of the primary cells into transformed lymphoblastoid cell lines (LCLs). These nascently infected B lymphocytes express a select set of latent genes one of which is the Epstein‐Barr nuclear antigen (EBNA) 3C essential for B cell transformation. The overall goals of this application is to characterize the involvement of EBNA3C with the well known tumor suppressor p53 and to determine the post translational modifications necessary for regulation of p53 which is induced by its interaction with EBNA3C. These studies are the first to directly testing the cooperation of p53 and an essential EBV nuclear antigen EBNA3C.