Biomechanical mapping of the optic nerve head and peripapillary sclera using high frequency ultrasonic elastography

使用高频超声弹性成像对视神经乳头和视乳头周围巩膜进行生物力学测绘

• 批准号: 10712180
• 负责人: Qifa Zhou
• 金额: $ 41.3万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10712180
• 关键词: 3-Dimensional; Acoustics; Address; Affect; Age; Alzheimer' s Disease; Alzheimer' s disease diagnosis; Alzheimer' s disease pathology; Alzheimer' s disease patient; American; Area; Award; Back; Biomechanics; Blindness; Blood Vessels; Blood capillaries; Blood flow; Brain; California; Cerebrovascular Disorders; Characteristics; Computer software; Cornea; Deposition; Development; Disease; Disease Progression; Early Diagnosis; Early Intervention; Economic Burden; Elasticity; Elements; Engineering; Evaluation; Eye; Frequencies; Glaucoma; Goals; Human; Image; Imaging Techniques; Individual; Knowledge; Maps; Measures; Mechanics; Microvascular Dysfunction; Monitor; Mus; Neurobehavioral Manifestations; Optic Disk; Oryctolagus cuniculus; Papillary; Parents; Pathogenesis; Pathologic; Patients; Penetration; Pericytes; Persons; Phase; Physiologic Intraocular Pressure; Physiological; Principal Investigator; Property; Race; Radiation; Research; Research Personnel; Resolution; Resources; Retina; Retinal Diseases; Risk Factors; Scientist; Sclera; Shapes; Site; Societies; Statistical Data Interpretation; Stress; System; Techniques; Technology; Testing; Tissues; Transducers; Ultrasonic Transducer; Ultrasonics; Ultrasonography; United States National Institutes of Health; Universities; Variant; Vascular Diseases; abeta deposition; aging population; behavior change; cerebral microvasculature; cerebrovascular; cost; density; disorder risk; elastography; ex vivo imaging; experience; imaging modality; imaging platform; imaging system; in vivo imaging system; in vivo optical imaging; mechanical properties; new technology; novel; optic nerve disorder; optical imaging; success; tool; ultrasound

Alzheimer's disease (AD) afflicts 6.5 million individuals in US and 15 million worldwide and elicits a huge economic burden to the society. However, there is no cure for AD and there is an urgent need to identify AD before occurrence of cognitive symptoms/irreversible pathological changes, thus allowing early interventions to delay the onset and progression of the disease. Cerebral vascular dysfunction is among the earliest pathological changes implicated in AD pathogenesis and a potential marker for early AD diagnosis, but the evaluation of the alterations in human cerebral vascular network function is limited by high cost, and low availability and sensitivity. Studies have demonstrated that many AD pathologies also occur in retina and back of eye, including Aβ deposition and microvascular dysfunction. Retinal and cerebral microvasculature share similar structural and physiological properties and can be directly imaged by OCTA. Indeed, vascular dysfunctions in AD retina has been detected in AD patients, including lower capillary density, reduced blood flow and pericyte loss, similar to cerebral vascular changes in AD. However, it remains largely unknown how the ONH and retrolaminar microvascular connecting to brain changes are related to other AD pathological changes because OCTA cannot penetrate back of retina. The goal of this proposal is to use high frequency ultrasound flow image to fill up these knowledge gaps by examining the longitudinal change of ONH and retrolaminar microvasculature in AD mice. There are two aims: Aim1 will examine the temporal changes of vascular function, including blood flow, capillary density, and pericyte loss in different pathological stages of AD mice (5XFAD) by high resolution ultrasound; Aim 2 will determine the relationship between the microvascular alterations and deposition in both brain and back of eye, BBB breakdown and behavior change in AD mice.

阿尔茨海默氏病（AD）折磨于美国的650万人，在全球范围内1500万人 经济焚烧社会。但是，无法治愈广告，迫切需要识别广告 发生认知症状/不可逆的病理变化之前 延迟疾病的发作和进展。脑血管功能障碍是最早的病理之一 与AD发病机理有关的变化和早期AD诊断的潜在标记，但评估 人脑血管网络功能的改变受到高成本，低可用性和敏感性的限制。 研究表明，许多AD病理也出现在视网膜和眼后，包括Aβ 沉积和微血管功能障碍。视网膜和脑微脉管系统具有相似的结构和 物理特性，可以由八颗直接成像。确实，AD视网膜中的血管功能障碍具有 在AD患者中检测到，包括较低的毛细血管密度，降低血流和周细胞损失，类似于 AD的脑血管变化。但是，它在很大程度上仍然未知如何 与大脑变化相连的微血管与其他AD病理变化有关，因为八 穿透视网膜。该建议的目的是使用高频超声流图像填充这些 通过检查AD小鼠中ONH和Retollaminar微脉管系统的纵向变化，知识差距。 有两个目的：AIM1将检查血管功能的暂时变化，包括血流，毛细管 高分辨率超声检查AD小鼠（5xFAD）的不同病理阶段的密度和周细胞损失；目的 2将确定大脑和背面的微血管改变与沉积之间的关系 AD小鼠的眼睛，BBB分解和行为改变。