SyncroPatch 384 Automated Patch Clamp Instrument

SyncroPatch 384 自动膜片钳仪器

• 批准号: 10721590
• 负责人: Jen Qian Pan
• 金额: $ 60万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-17 至 2024-07-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721590
• 关键词: 7 year old; Amplifiers; Area; Biological Assay; Boston; Closure by clamp; Data Analyses; Electrophysiology (science); Funding; Genetic Diseases; Head; Human; Individual; Institution; Ion Channel; Liquid substance; Manuals; Measurement; National Institute of Mental Health; Neurons; Operating System; Patch-Clamp Techniques; Pharmacology Study; Property; Research Personnel; Research Project Grants; Resistance; Robotics; Running; Scientist; Technology; Temperature; Variant; cell type; drug discovery; instrument; manufacture; patch clamp; seal; success; user friendly software; voltage clamp

The objective of this proposal is to enhance the capability of a diverse group of researchers funded by National Institute of Mental Health in the greater Boston area to perform electrophysiological assays for the purposes of drug discovery, targeted pharmacological studies, determining the functional consequences of human ion channel variants associated with genetic diseases, and investigating the electrophysiological properties of human neurons. This objective will be achieved by the purchase and installation of a 384-well automated planar patch clamp instrument (SyncroPatch 384) manufactured by Nanion Technologies. This instrument will replace the 7-year-old legacy instrument (SyncroPatch 384 PE) this is approaching obsolescence and lacks critically important features that are needed by researchers. The Nanion SyncroPatch 384 instrument consists of a patch clamp module (measurement chamber) integrated with a state-of-the-art robotic liquid handler with a 384-pipetting head (Biomek i5), 384 individual patch clamp amplifiers that can operate in either voltage-clamp or current-clamp mode, advanced temperature control with range 10°C to 37°C, and powerful user-friendly software for instrument control and data analysis that runs under a supported operating system. The instrument allows for formation of giga-Ohm resistance seals with a variety of cell types and produces electrophysiological recordings with high fidelity that are comparable to the traditional manual patch clamp technique but at two orders of magnitude greater throughput. The instrument will be managed by the ICE-T (ion channel and electrogenic transporter) platform at Broad Institute. Strong institutional support for the instrument and its deployment will be provided, and effective oversight will be achieved by a team of professional scientist. The instrument is expected to enhance the success of several NIMH - funded research projects and provide a new platform for active and future research.

这项建议的目的是提高由以下机构资助的各种研究人员的能力： 美国国家精神卫生研究所在大波士顿地区进行电生理分析， 药物发现的目的，有针对性的药理学研究， 与遗传疾病相关的人类离子通道变异的后果，并调查 人类神经元的电生理特性。这一目标将通过购买和 安装384孔自动化平面膜片钳仪器（SyncroPatch 384）， Nanion Technologies.该仪器将取代已有7年历史的旧仪器（SyncroPatch 384 PE）这是接近过时，缺乏所需的至关重要的功能， 研究人员Nanion SyncroPatch 384仪器由膜片钳模块（测量膜片钳的电压）组成。 腔室）与具有384-移液头（Biomek i5）的最先进的机器人液体处理器集成， 384个独立的膜片钳放大器，可以在电压钳或电流钳模式下工作， 先进的温度控制范围为10°C至37°C，以及功能强大的用户友好型软件， 在支持的操作系统下运行的仪器控制和数据分析。仪器 允许与各种电池类型形成千兆欧姆电阻密封， 高保真度的电生理记录，可与传统的手动贴片相媲美 钳位技术，但在两个数量级更大的吞吐量。该仪器将由 Broad Institute的ICE-T（离子通道和生电转运蛋白）平台。坚强体制 将为该工具及其部署提供支持，并通过以下方式实现有效监督 一队专业科学家该仪器预计将促进几个NIMH的成功- 资助研究项目，并为积极和未来的研究提供新的平台。