SyncroPatch 384 Automated Patch Clamp Instrument

SyncroPatch 384 自动膜片钳仪器

• 批准号: 10721590
• 负责人: Jen Qian Pan
• 金额: $ 60万
• 依托单位: BROAD INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-17 至 2024-07-16
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721590
• 关键词: 7 year old; Amplifiers; Area; Biological Assay; Boston; Closure by clamp; Data Analyses; Electrophysiology (science); Funding; Genetic Diseases; Head; Human; Individual; Institution; Ion Channel; Liquid substance; Manuals; Measurement; National Institute of Mental Health; Neurons; Operating System; Patch-Clamp Techniques; Pharmacology Study; Property; Research Personnel; Research Project Grants; Resistance; Robotics; Running; Scientist; Technology; Temperature; Variant; cell type; drug discovery; instrument; manufacture; patch clamp; seal; success; user friendly software; voltage clamp

The objective of this proposal is to enhance the capability of a diverse group of researchers funded by National Institute of Mental Health in the greater Boston area to perform electrophysiological assays for the purposes of drug discovery, targeted pharmacological studies, determining the functional consequences of human ion channel variants associated with genetic diseases, and investigating the electrophysiological properties of human neurons. This objective will be achieved by the purchase and installation of a 384-well automated planar patch clamp instrument (SyncroPatch 384) manufactured by Nanion Technologies. This instrument will replace the 7-year-old legacy instrument (SyncroPatch 384 PE) this is approaching obsolescence and lacks critically important features that are needed by researchers. The Nanion SyncroPatch 384 instrument consists of a patch clamp module (measurement chamber) integrated with a state-of-the-art robotic liquid handler with a 384-pipetting head (Biomek i5), 384 individual patch clamp amplifiers that can operate in either voltage-clamp or current-clamp mode, advanced temperature control with range 10°C to 37°C, and powerful user-friendly software for instrument control and data analysis that runs under a supported operating system. The instrument allows for formation of giga-Ohm resistance seals with a variety of cell types and produces electrophysiological recordings with high fidelity that are comparable to the traditional manual patch clamp technique but at two orders of magnitude greater throughput. The instrument will be managed by the ICE-T (ion channel and electrogenic transporter) platform at Broad Institute. Strong institutional support for the instrument and its deployment will be provided, and effective oversight will be achieved by a team of professional scientist. The instrument is expected to enhance the success of several NIMH - funded research projects and provide a new platform for active and future research.

这项提议的目标是提高由以下资助的不同研究人员群体的能力 在大波士顿地区的国家精神卫生研究所为 药物发现的目的，有针对性的药理学研究，确定功能 与遗传病相关的人类离子通道变异的后果，并调查 人类神经元的电生理特性。这一目标将通过购买和 安装由制造的384孔自动平面膜片钳仪器(SyncroPatch 384) 奈米恩科技公司。此仪器将取代已有7年历史的传统仪器(SyncroPatch 384 PE)这正在接近过时，并且缺乏以下所需的至关重要的功能 研究人员。Nanion SyncroPatch 384仪器由膜片钳模块(测量 室)与最先进的机器人液体处理机集成在一起，带有384个吸头(Biomek I5)， 384个可工作在电压钳位或电流钳位模式下的单个膜片钳放大器， 10°C至37°C范围内的高级温度控制，以及功能强大的用户友好型软件 在支持的操作系统下运行的仪器控制和数据分析。这台仪器 允许形成具有各种电池类型和产品的千兆欧姆电阻密封件 高保真的电生理记录，可与传统的手动贴片相媲美 钳位技术，但吞吐量提高了两个数量级。该仪器将由 布罗德研究所的ICE-T(离子通道和生电转运体)平台。强大的机构 将为该文书及其部署提供支持，并将通过以下方式实现有效监督 一支专业的科学家团队。预计该仪器将促进几个NIMH的成功- 资助研究项目，并为积极和未来的研究提供新的平台。