Keratinocyte Core

角质形成细胞核心

• 批准号: 7677671
• 负责人: Kathleen Janee Green
• 金额: $ 18.93万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7677671
• 关键词: Adhesions; Aerobic; Area; Biology; Boxing; Cataloging; Catalogs; Cell Culture System; Cell Culture Techniques; Cells; Collaborations; Collagen; Communities; Consultations; Culture Techniques; Defect; Dermatology; Development; Disease; Elements; Engineering; Epidermis; Epithelial; Epithelial Cells; Equipment; Evaluation; Experimental Designs; Factor Analysis; Fostering; Goals; Human; Human papillomavirus 16; Hypoxia; Investigation; Life; Maintenance; Methods; Microscope; Modeling; Molecular; Monitor; Mus; Pathology; Patients; Preparation; Process; Reagent; Research; Research Personnel; Resources; Retroviridae; Safety; Sampling; Services; Signal Transduction; Skin; Stratified Epithelium; System; Techniques; Technology; Testing; Time; Training; Ultraviolet B Radiation; Universities; Wound Healing; base; cell motility; cellular imaging; cost; design; efficacy testing; genetic manipulation; irradiation; keratinocyte; member; novel; skills training; skin disorder; tissue culture; tissue/cell culture; ultraviolet irradiation

Progress in our understanding of skin biology and disease has been greatly advanced by the use of epidermal cell cultures. The Keratinocyte Core is designed to support and attract new investigators in skin biology by providing a reliable supply of highly purified preparations of human and mouse epidermal keratinocytes at a reduced cost. In addition, the Core will train researchers in the development and use of these various cell culture systems. In cases where more permanent cultures are required, immortalization and long-term culture techniques will be applied to human and mouse keratinocytes. The Core will serve as a resource for: 1) storing these cells and bulk tissue culture supplies; 2) transmitting expertise and techniques for studying keratinocytes; and 3) providing access to larger equipment suitable for keratinocyte biology (e.g., a UV irradiation lamp box, a humidified variable aerobic workstation for hypoxia studies, and a Nikon inverted microscope integrated for live cell imaging of linear scratch wounds). The Core will also provide training and supplies for a three-dimensional organotypic model for human epidermis that permits analysis of factors controlling the formation and organization of this stratified epithelium during development and in wound healing processes. Collectively, the Core will offer the necessary materials and expertise for NU SDRC investigators to better develop novel cell and molecular based strategies towards curing skin diseases and will help foster new collaborations among groups with the common goal of understanding the biology of epithelial cells.

在我们对皮肤生物学和疾病的理解方面，通过使用 表皮细胞培养。角质形成细胞核心的设计是为了支持和吸引皮肤领域的新研究人员 通过提供可靠的人和小鼠表皮高纯度制剂的生物学 角质形成细胞以更低的成本。此外，核心将培训研究人员开发和使用 这些不同的细胞培养系统。在需要更持久文化的情况下，永垂不朽 长期培养技术将应用于人和小鼠的角质形成细胞。核心将作为 一种资源：1)储存这些细胞和大量组织培养用品；2)传播专业知识和 研究角质形成细胞的技术；以及3)提供适合角质形成细胞的更大设备的通道 生物学(例如，紫外线照射灯箱、用于低氧研究的加湿可变有氧工作站和 尼康倒置显微镜集成，用于线性划痕伤口的活细胞成像)。核心还将 为人体表皮的三维器官模型提供培训和用品 发育过程中控制复层上皮形成和组织的因素分析 以及在伤口愈合过程中。总体而言，核心将提供必要的材料和专业知识 NU SDRC调查人员将更好地开发基于细胞和分子的新策略来治疗皮肤 并将有助于促进团体之间的新合作，其共同目标是了解 上皮细胞生物学。