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Core B STEM

核心B STEM

基本信息

批准号：
10259797
负责人：
Kathleen Janee Green
金额：
$ 22.13万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10259797
关键词：
3-Dimensional Address Adult Age Architecture Archives Automobile Driving Basic Science Biological Assay Biology Biomedical Engineering Cell Culture Techniques Cell Separation Cell model Cells Cellular Morphology Chemistry Client Collaborations Consultations Culture Media Custom Cutaneous Data Analyses Defect Derivation procedure Dermatology Development Discipline Disease Engineering Epidermis Epithelial Equipment Evaluation Experimental Designs Female Fibroblasts Fresh Tissue Gender Gene Expression Genetic Goals Health Service Area Homeostasis Human Hypersensitivity Imaging technology Immune Immunology Individual Investigation Investigational Drugs Knockout Mice Knowledge Laboratory Research Libraries Longevity Maintenance Methodology Methods Microscope Microscopy Modeling Molecular Morphology Mus Neonatal Neurons Patients Pharmacology Phenotype Physiological Procedures Proteomics Protocols documentation Race Reader Reagent Reporter Research Research Personnel Resources Sampling Services Site Skin Skin Tissue Source Stains Standardization System Technology Therapeutic Tissue Engineering Tissue Sample Tissues Training Transgenic Mice Transglutaminases Translating Translational Research Ultraviolet B Radiation Universities Viral analysis pipeline antibody libraries base biobank cell bank cell immortalization cell type cost demographics drug discovery histological stains human disease human model induced pluripotent stem cell innovation instrument involucrin keratinocyte live cell imaging male mechanical properties melanocyte member mouse model multidisciplinary novel penis foreskin programs promoter protein expression reconstitution repository research and development skin disorder skin morphogenesis three dimensional cell culture three-dimensional modeling tissue culture tool translational study virology

项目摘要

Skin culture models serve as a key tool for understanding normal and diseased skin in well-controlled culture systems that empower investigators to probe skin biology with a rigorous model for mechanistic analysis. 3D skin culture models using primary cells are especially powerful as they closely mimic skin morphogenesis, differentiation, and mechanical properties, leading to a physiologically-relevant tissue architecture. This model is particularly applicable to study mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering and Morphology (STEM) Core enables SBDRC researchers to apply primary skin culture models in their research program. Towards this aim, the STEM Core provides training, services, specialized equipment, and materials for the initiation, maintenance, processing, and analysis of primary human skin cell cultures, i.e., keratinocytes, melanocytes, fibroblasts, neurons, and immune cells (with the TEST IT Core). The STEM Core also generates short- and long-term mouse keratinocyte cultures for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin, female and male adult skin, and a library of patient keratinocytes. We endeavor to maintain a diverse cell bank with donors of various demographics (age, gender, race, body site, and disease). In collaboration with GET iN Core, the STEM Core optimized methods for viral, pharmacological, and genetic reprogramming of primary skin cells, including induced pluripotent stem cells (iPSCs). Our efforts will also focus on investigator-driven research and development to customize 3D cultures and extend their applicability to address multiple aspects of skin biology from a variety of disciplines including allergy, drug discovery, materials engineering, and chemistry. Training and provision of 3D organotypic models of human epidermis established from control and patient skin cells allows investigators to address skin biology in an architecturally appropriate manner where multiple cell types can interact with one another.

皮肤培养模型是了解正常和病变皮肤在良好控制培养中的关键工具