Core B STEM

皮肤培养模型是在良好控制的培养中理解正常皮肤和患病皮肤的关键工具 使研究人员能够探索皮肤生物学的系统，具有严格的机械分析模型。3D 使用原代细胞的皮肤培养模型特别强大，因为它们接近于模拟皮肤的形态发生， 分化和机械特性，导致与生理相关的组织结构。这款车 特别适用于研究管理组织发育、动态平衡和疾病的机制。这个 皮肤组织工程和形态(STEM)核心使SBDRC的研究人员能够应用初级皮肤 在他们的研究计划中建立文化模型。为实现这一目标，STEM核心提供培训、服务、 用于启动、维护、处理和分析原始人的专门设备和材料 皮肤细胞培养，即角质形成细胞、黑素细胞、成纤维细胞、神经元和免疫细胞(含检测IT 核心)。STEM Core还为旨在研究的小鼠角质形成细胞提供短期和长期培养 确定皮肤缺陷的细胞和分子基础，这在工程小鼠模型中很明显。用户有权访问 从新生儿包皮、女性和男性成人皮肤中分离出大量原代人类角质形成细胞， 和一个病人角质形成细胞库。我们努力维持一个多元化的细胞库，捐赠者来自不同的国家 人口统计数据(年龄、性别、种族、身体部位和疾病)。与Get In Core合作，STEM Core 对原代皮肤细胞进行病毒、药物和遗传重编程的优化方法，包括诱导 多能干细胞(IPSCs)。我们的努力还将集中在调查人员驱动的研究和开发上，以 自定义3D文化并扩展其适用性，以解决皮肤生物学的多个方面 学科包括过敏、药物发现、材料工程和化学。培训和提供3D 从对照和患者皮肤细胞建立的人类表皮器官模型使研究人员能够 以架构上合适的方式解决皮肤生物学问题，其中多种细胞类型可以与一种细胞相互作用 又一个。

Skin culture models serve as a key tool for understanding normal and diseased skin in well-controlled culture systems that empower investigators to probe skin biology with a rigorous model for mechanistic analysis. 3D skin culture models using primary cells are especially powerful as they closely mimic skin morphogenesis, differentiation, and mechanical properties, leading to a physiologically-relevant tissue architecture. This model is particularly applicable to study mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering and Morphology (STEM) Core enables SBDRC researchers to apply primary skin culture models in their research program. Towards this aim, the STEM Core provides training, services, specialized equipment, and materials for the initiation, maintenance, processing, and analysis of primary human skin cell cultures, i.e., keratinocytes, melanocytes, fibroblasts, neurons, and immune cells (with the TEST IT Core). The STEM Core also generates short- and long-term mouse keratinocyte cultures for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin, female and male adult skin, and a library of patient keratinocytes. We endeavor to maintain a diverse cell bank with donors of various demographics (age, gender, race, body site, and disease). In collaboration with GET iN Core, the STEM Core optimized methods for viral, pharmacological, and genetic reprogramming of primary skin cells, including induced pluripotent stem cells (iPSCs). Our efforts will also focus on investigator-driven research and development to customize 3D cultures and extend their applicability to address multiple aspects of skin biology from a variety of disciplines including allergy, drug discovery, materials engineering, and chemistry. Training and provision of 3D organotypic models of human epidermis established from control and patient skin cells allows investigators to address skin biology in an architecturally appropriate manner where multiple cell types can interact with one another.