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Core B STEM

核心B STEM

基本信息

批准号：
10700041
负责人：
Kathleen Janee Green
金额：
$ 20.7万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-20 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10700041
关键词：
3-Dimensional Address Adult Age Architecture Archives Automobile Driving Basic Science Biological Assay Biology Biomedical Engineering Cell Culture Techniques Cell Separation Cell model Cells Cellular Morphology Chemistry Client Collaborations Consultations Culture Media Cutaneous Data Analyses Defect Derivation procedure Dermatology Development Discipline Disease Engineering Epidermis Epithelium Equipment Evaluation Experimental Designs Female Fibroblasts Fresh Tissue Gender Gene Expression Genetic Glutaminase Goals Health Service Area Homeostasis Human Hypersensitivity Imaging technology Immune Immunology Individual Investigation Knockout Mice Knowledge Laboratory Research Libraries Light Longevity Maintenance Methodology Methods Microscope Microscopy Modeling Molecular Morphology Mus Neonatal Neurons Patients Phenotype Physiological Procedures Proteomics Protocols documentation Race Reader Reagent Reporter Research Research Personnel Resources Sampling Services Site Skin Skin Tissue Source Stains Standardization System Technology Therapeutic Tissue Engineering Tissue Sample Tissues Training Transgenic Organisms Translating Translational Research Ultraviolet B Radiation Universities Viral analysis pipeline antibody libraries base biobank cell bank cell immortalization cell type cost demographics drug discovery empowerment histological stains human disease human model induced pluripotent stem cell innovation instrument involucrin keratinocyte live cell imaging male mechanical properties melanocyte member mouse model multidisciplinary novel penis foreskin pharmacologic programs promoter protein expression reconstitution repository research and development skin disorder skin morphogenesis three dimensional cell culture three-dimensional modeling tissue culture tool translational study virology

项目摘要

Skin culture models serve as a key tool for understanding normal and diseased skin in well-controlled culture systems that empower investigators to probe skin biology with a rigorous model for mechanistic analysis. 3D skin culture models using primary cells are especially powerful as they closely mimic skin morphogenesis, differentiation, and mechanical properties, leading to a physiologically-relevant tissue architecture. This model is particularly applicable to study mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering and Morphology (STEM) Core enables SBDRC researchers to apply primary skin culture models in their research program. Towards this aim, the STEM Core provides training, services, specialized equipment, and materials for the initiation, maintenance, processing, and analysis of primary human skin cell cultures, i.e., keratinocytes, melanocytes, fibroblasts, neurons, and immune cells (with the TEST IT Core). The STEM Core also generates short- and long-term mouse keratinocyte cultures for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin, female and male adult skin, and a library of patient keratinocytes. We endeavor to maintain a diverse cell bank with donors of various demographics (age, gender, race, body site, and disease). In collaboration with GET iN Core, the STEM Core optimized methods for viral, pharmacological, and genetic reprogramming of primary skin cells, including induced pluripotent stem cells (iPSCs). Our efforts will also focus on investigator-driven research and development to customize 3D cultures and extend their applicability to address multiple aspects of skin biology from a variety of disciplines including allergy, drug discovery, materials engineering, and chemistry. Training and provision of 3D organotypic models of human epidermis established from control and patient skin cells allows investigators to address skin biology in an architecturally appropriate manner where multiple cell types can interact with one another.

皮肤培养模型是在良好控制的培养中了解正常和患病皮肤的关键工具使研究人员能够通过严格的机械分析模型来探索皮肤生物学的系统。 3D 使用原代细胞的皮肤培养模型特别强大，因为它们非常模仿皮肤形态发生，分化和机械特性，形成生理相关的组织结构。这个型号特别适用于研究控制组织发育、体内平衡和疾病的机制。这皮肤组织工程和形态学 (STEM) 核心使 SBDRC 研究人员能够应用初级皮肤他们的研究计划中的文化模型。为了实现这一目标，STEM 核心提供培训、服务、用于初级人类的启动、维护、处理和分析的专用设备和材料皮肤细胞培养物，即角质细胞、黑素细胞、成纤维细胞、神经元和免疫细胞（使用 TEST IT 核）。 STEM Core 还生成短期和长期小鼠角质形成细胞培养物，用于旨在定义工程小鼠模型中明显皮肤缺陷的细胞和分子基础。用户有权访问从新生儿包皮、女性和男性成人皮肤中分离出大量原代人类角质形成细胞，和患者角质形成细胞库。我们努力维持一个多元化的细胞库，其中包括来自不同领域的捐赠者人口统计（年龄、性别、种族、身体部位和疾病）。与 GET iN Core 合作，STEM 核心原代皮肤细胞的病毒、药理学和遗传重编程的优化方法，包括诱导多能干细胞（iPSC）。我们的努力还将集中于研究者驱动的研究和开发定制 3D 培养物并扩展其适用性，以解决皮肤生物学的多个方面的问题学科包括过敏、药物发现、材料工程和化学。培训和提供 3D 从对照和患者皮肤细胞建立的人类表皮器官模型使研究人员能够以一种结构上适当的方式解决皮肤生物学问题，其中多种细胞类型可以与一种细胞相互作用其他。