Core B STEM

皮肤培养模型作为一个关键的工具，了解正常和患病的皮肤在良好的控制文化 该系统使研究人员能够通过严格的机制分析模型来探测皮肤生物学。3D 使用原代细胞的皮肤培养模型是特别有效的，因为它们紧密地模拟皮肤形态发生， 分化和机械性能，导致生理相关的组织结构。该模型 特别适用于研究支配组织发育、体内平衡和疾病的机制。的 皮肤组织工程和形态学（STEM）核心使SBDRC研究人员能够将原代皮肤 在他们的研究计划中的文化模式。为了实现这一目标，STEM核心提供培训，服务， 用于启动、维护、处理和分析初级人类活动的专用设备和材料。 皮肤细胞培养物，即，角质形成细胞，黑色素细胞，成纤维细胞，神经元和免疫细胞（与测试它 核心）。STEM Core还生成短期和长期小鼠角质形成细胞培养物，用于以下研究： 定义了在工程小鼠模型中明显的皮肤缺陷的细胞和分子基础。用户可以访问 从新生儿包皮，女性和男性成人皮肤， 和患者角质形成细胞文库。我们奋进维持一个多样化的细胞库， 人口统计学（年龄、性别、种族、身体部位和疾病）。与GET iN Core合作，STEM Core 用于原代皮肤细胞的病毒、药理学和遗传重编程的优化方法，包括诱导的 多能干细胞（iPSC）。我们的努力还将集中在发电机驱动的研究和开发， 定制3D培养物并扩展其适用性，以解决来自各种皮肤生物学的多个方面， 学科包括过敏，药物发现，材料工程和化学。培训和提供3D 从对照和患者皮肤细胞建立的人表皮器官型模型使研究人员能够 以适当的结构方式解决皮肤生物学问题，其中多种细胞类型可以与一种细胞相互作用 另

Skin culture models serve as a key tool for understanding normal and diseased skin in well-controlled culture systems that empower investigators to probe skin biology with a rigorous model for mechanistic analysis. 3D skin culture models using primary cells are especially powerful as they closely mimic skin morphogenesis, differentiation, and mechanical properties, leading to a physiologically-relevant tissue architecture. This model is particularly applicable to study mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering and Morphology (STEM) Core enables SBDRC researchers to apply primary skin culture models in their research program. Towards this aim, the STEM Core provides training, services, specialized equipment, and materials for the initiation, maintenance, processing, and analysis of primary human skin cell cultures, i.e., keratinocytes, melanocytes, fibroblasts, neurons, and immune cells (with the TEST IT Core). The STEM Core also generates short- and long-term mouse keratinocyte cultures for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin, female and male adult skin, and a library of patient keratinocytes. We endeavor to maintain a diverse cell bank with donors of various demographics (age, gender, race, body site, and disease). In collaboration with GET iN Core, the STEM Core optimized methods for viral, pharmacological, and genetic reprogramming of primary skin cells, including induced pluripotent stem cells (iPSCs). Our efforts will also focus on investigator-driven research and development to customize 3D cultures and extend their applicability to address multiple aspects of skin biology from a variety of disciplines including allergy, drug discovery, materials engineering, and chemistry. Training and provision of 3D organotypic models of human epidermis established from control and patient skin cells allows investigators to address skin biology in an architecturally appropriate manner where multiple cell types can interact with one another.