Stem Cell and Xenografts

干细胞和异种移植物

• 批准号: 7678119
• 负责人: GEORGE COTSARELIS
• 金额: $ 14.7万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7678119
• 关键词: Biological Assay; Biology; Cell Separation; Cells; Cutaneous; Development; Environment; Epithelial; Flow Cytometry; Funding; Goals; Hair follicle structure; Human; Immunodeficient Mouse; Immunohistochemistry; Institutes; Label; Laboratories; Laboratory Personnel; Medicine; Mus; Mutant Strains Mice; Pathology; Population; Regenerative Medicine; Research; Research Personnel; Resources; Running; Services; Skin; Skin Transplantation; Skin graft; Stem cells; Techniques; Technology; Testing; Tissues; Training; Transgenic Mice; Transgenic Organisms; Translations; Work; Xenograft Model; Xenograft procedure; clinically relevant; cost effective; in vivo; interest; medical schools; member; mutant; pre-clinical; prevent; reconstitution; skin xenograft; stem; stem cell population

Core C will support and encourage research into cutaneous epithelial stem cells by providing expertise and training in the characterization and isolation of epithelial stem cells in the hair follicle. The main goals of Core C are to enable investigators to analyze their transgenic or mutant mice for alterations in epithelial stem cells and to encourage translation of mouse work to human skin using a human skin/scid mouse xenograft model. Core C takes advantage of assays and expertise in stem cells found in the core director's laboratory, as well as existing resources and successful cores established at Penn. In particular, Core C will integrate with Penn's School of Medicine Flow Cytometry Core run out of the pathology department and the Xenografting core in the department of medicine that is partly funded through the Institute for Regenerative Medicine. These interactions will assure that investigators will have access to high quality, cost effective services, as well as the scientific expertise necessary to study epithelial stem cells in their research efforts. The stem cell core will offer analysis of hair follicle stem and progenitor cells using a wide variety of standard (label-retaining cell, immunohistochemistry) and cutting edge assays (marker analysis by fluorescent activated cell sorting [FACS],.multipotency testing). Core C will offer functional testing of hair follicle stem and progenitor cells in reconstitution assays. A major part of the core will be to provide training to laboratory personnel so that they can isolate cell populations from the skin and perform FACS analysis and cell isolation using the established Flow Cytometry Core. The human skin xenograft portion of Core C will encourage testing relevance of mouse findings in human skin. The xenograft model is an elegant means to perform preclinical functional studies on human skin that will streamline the development of clinically relevant technologies. Core C will integrate with Core A by using Core A's immunohistochemistry services to assess stem cell markers in tissues. Core C will integrate with Core B by receiving skin for xenografting.

核心C将支持和鼓励研究皮肤上皮干细胞，提供专业知识， 毛囊中上皮干细胞的鉴定和分离培训。核心的主要目标 C是使研究人员能够分析他们的转基因或突变小鼠的上皮干细胞的变化 并鼓励使用人皮肤/SCID小鼠异种移植模型将小鼠工作转化为人皮肤。 核心C也利用了核心主任实验室中发现的干细胞检测和专业知识 作为现有的资源和成功的核心建立在宾夕法尼亚大学。特别是，核心C将与 宾夕法尼亚大学医学院流式细胞术核心运行出病理部门和异种移植 医学系的核心，部分资金来自再生医学研究所。 这些互动将确保研究者能够获得高质量、具有成本效益的服务， 以及在他们的研究工作中研究上皮干细胞所必需的科学专业知识。 干细胞核心将提供毛囊干细胞和祖细胞的分析，使用各种各样的标准， （标记保留细胞，免疫组织化学）和尖端测定（荧光标记分析） 活化细胞分选[FACS]，多能性测试）。核心C将提供毛囊干的功能测试 和祖细胞。核心的一个主要部分将是向实验室提供培训 这样他们就可以从皮肤中分离细胞群，并进行FACS分析和细胞培养。 使用已建立的流式细胞术核心进行分离。 核心C的人皮肤异种移植物部分将鼓励在人类中测试小鼠发现的相关性 皮肤异种移植模型是对人类皮肤进行临床前功能研究的一种优雅手段， 将简化临床相关技术的开发。 核心C将通过使用核心A的免疫组织化学服务来评估干细胞， 组织中的标记物。核心C将通过接受用于异种移植的皮肤与核心B整合。