Optically mapping tissue biomechanics during neural tube closure

神经管闭合过程中光学映射组织生物力学

• 批准号: 10790936
• 负责人: Jitao Zhang
• 金额: $ 11.4万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10790936
• 关键词: 3-Dimensional; Address; Advanced Development; Affect; Apical; Atomic Force Microscopy; Award; Biochemical; Biological; Biology; Biomechanics; Biomedical Research; Biophysics; Calibration; Cells; Complement; Complex; Congenital Abnormality; Cues; Data; Defect; Detection; Development; Developmental Biology; Developmental Diagnostic; Dorsal; Elastic Tissue; Elasticity; Embryo; Embryonic Development; Ensure; Environmental Risk Factor; Event; Evolution; F-Actin; Failure; Gases; Gene Combinations; Gene Expression; Genetic; Goals; Grant; Hour; Human; In Situ; Knowledge; Lighting; Link; Maps; Measurement; Measures; Mechanics; Methods; Microscopic; Microscopy; Modeling; Modulus; Monitor; Morphogenesis; Morphology; Mus; Names; Neural Fold; Neural Tube Closure; Neural Tube Defects; Neural tube; Noise; Optics; Pattern; Phenotype; Pregnancy; Process; Property; Recovery; Regulation; Research; Research Personnel; Resistance; Resolution; Role; Sample Size; Sampling; Scanning; Science; Shapes; Signal Transduction; Speed; Spinal Dysraphism; Techniques; Technology; Testing; Therapeutic; Time; Tissue Engineering; Tissues; Training; absorption; biomechanical engineering; cell behavior; constriction; convergent extension; driving force; elastography; embryo tissue; imaging modality; improved; in vivo; innovation; instrument; light scattering; mechanical properties; mechanical stimulus; mutant mouse model; neural; neural plate; novel; photonics; skills; technology development; technology research and development; technology validation; tissue mapping

Project Summary This project features the development of advanced photonic technology to attack a major unmet challenge in developmental biology. Embryonic morphogenesis results from a complex combination of gene expression, biochemical signaling, and biomechanics. While methods to evaluate the first two are well established, our knowledge of biomechanics of the embryo morphogenesis is poorly understood because of the lack of technical approaches. Elucidating the biomechanics underlying morphogenesis is essential towards understanding the interplay between mechanical regulation, gene expression and tissue patterning that drive embryogenesis, and will potentially lead to exciting innovations in therapeutic strategies and diagnostics for developmental defects. Current technology for tissue elasticity measurement is slow and invasive, thus cannot measure mechanical properties within living 3D embryonic tissue in-situ. This project will develop an all-optical approach (line-scanning Brillouin microscopy, LSBM) to fulfill this unmet need. LSBM allows rapid 3D mapping of the elasticity of embryonic tissue in-situ with high-resolution, non-invasive, and non-contact manner. After technology validation, I will use this technique to address an open question of the development field related to the role of tissue biomechanics in the process of neural tube closure. The central hypothesis of this grant is that the neural tube defect is related to the altered mechanical properties of tissue. Specifically, I will investigate the role of cellular activities, such as apical constriction, in the stiffness change of tissue during different stages of neurulation and specific genetic factors contribute to the abnormal changes in tissue stiffness. This K25 award, through its training and research components, will provide me with the skills to create a strong biological part in my future research, in which I will utilize the enabling technological capabilities to address the important needs in developmental biology. The overall effort will hasten my transition to being an independent investigator at the forefront of the interdisciplinary interface of technology development and biomedical research.

项目总结

项目成果

Publisher Correction: Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy.

出版商更正：通过双线扫描布里渊显微镜在低照射水平下快速生物力学成像。

• DOI: 10.1038/s41592-023-01877-0
• 发表时间: 2023
• 期刊: Nature methods
• 影响因子: 48
• 作者: Zhang,Jitao;Nikolic,Milos;Tanner,Kandice;Scarcelli,Giuliano
• 通讯作者: Scarcelli,Giuliano

A Novel Role of Connective Tissue Growth Factor in the Regulation of the Epithelial Phenotype.

• DOI: 10.3390/cancers15194834
• 发表时间: 2023-10-02
• 期刊: Cancers
• 影响因子: 5.2
• 作者: Gogoi RP;Galoforo S;Fox A;Morris C;Ramos H;Gogoi VK;Chehade H;Adzibolosu NK;Shi C;Zhang J;Tedja R;Morris R;Alvero AB;Mor G
• 通讯作者: Mor G

Non-contact and label-free biomechanical imaging: Stimulated Brillouin microscopy and beyond.

非接触式、无标记生物力学成像：受激布里渊显微镜及其他。

• DOI: 10.3389/fphy.2023.1175653
• 发表时间: 2023
• 期刊: Frontiers in physics
• 影响因子: 3.1
• 作者: Shi,Chenjun;Zhang,Hongyuan;Zhang,Jitao
• 通讯作者: Zhang,Jitao