Administrative Supplements for Equipment Purchases

设备采购行政补充

• 批准号: 10794680
• 负责人: Mi Hye Song
• 金额: $ 9.99万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794680
• 关键词: 26S proteasome; Administrative Supplement; Affect; Award; Biochemical; Biological Models; Biology; Brain; CRISPR/Cas technology; Caenorhabditis elegans; Cell Cycle; Cell division; Centrioles; Centrosome; Compensation; Defect; Detection; Development; Disease; Embryo; Ensure; Equipment; Exhibits; Funding; Genetic; Genomic Instability; Goals; Healthcare; Homeostasis; Human; Knowledge; Malignant Neoplasms; Measurement; Mediating; Microcephaly; Microtubule-Organizing Center; Mus; Mutation; National Institute of General Medical Sciences; Parents; Predisposition; Protein Analysis; Proteins; Proteolysis; Quantitative Microscopy; Regulation of Proteolysis; Request for Proposals; Research; Resolution; Role; Signal Transduction; System; Testing; Therapeutic Intervention; Work; anaphase-promoting complex; ciliopathy; daughter cell; equipment acquisition; genome editing; genome integrity; human disease; imaging system; in vivo; in vivo Model; mutant; nano; parent project; recruit; success; transmission process; tumorigenesis; ubiquitin ligase; ubiquitin-protein ligase

Project Summary Under the current NIGMS R15 research award (1R15GM147857-01), this proposal requests supplemental equipment funds that will be used to replace an old broken Odyssey Infrared Imaging system. The parent project focuses on the Proteolytic Regulation of Centrosome Proteins. The requested equipment is essential for carrying out the experimental plans for the Quantitative and Qualitative analysis of proteins successfully. The accurate measurement of protein levels and detection of proteins in low abundance require enhanced sensitivity and resolution. This administrative supplement fund will allow us to purchase Odyssey M Imaging system. This new imaging system will replace a broken Odyssey Infrared scanner and offer technical upgrades in the sensitivity and resolution of signal detection, which will serve a critical role in the success of the parent R15 project.

项目摘要 根据当前的NIGMS R15研究奖(1R15GM147857-01)，本提案要求 补充设备资金，将用于更换旧的损坏的奥德赛红外线 成像系统。母项目的重点是中心体的蛋白分解调节 蛋白质。所要求的设备是执行以下试验计划所必需的 成功地对蛋白质进行了定性和定量分析。准确的 蛋白质水平的测量和低丰度蛋白质的检测需要加强 敏感度和分辨率。这笔行政补充基金将允许我们收购奥德赛 M成像系统。这一新的成像系统将取代损坏的奥德赛红外扫描仪 并在信号检测的灵敏度和分辨率方面提供技术升级，这将服务于 在母公司R15项目的成功中发挥了关键作用。

项目成果

Site-specific phosphorylation of ZYG-1 regulates ZYG-1 stability and centrosome number.

• DOI: 10.1016/j.isci.2023.108410
• 发表时间: 2023-12-15
• 期刊: ISCIENCE
• 影响因子: 5.8
• 作者: Medley, Jeffrey C.;Yim, Rachel N.;DiPanni, Joseph;Sebou, Brandon;Shaffou, Blake;Cramer, Evan;Wu, Colin;Kabara, Megan;Song, Mi Hye
• 通讯作者: Song, Mi Hye

kin-3 genetically suppresses sur-6 in centrosome assembly during Caenorhabditis elegans embryogenesis.

• DOI: 10.17912/micropub.biology.000791
• 发表时间: 2023
• 期刊: microPublication biology
• 作者: Medley, Jeffrey C;Song, Mi Hye
• 通讯作者: Song, Mi Hye