STRUCT CHAR OF PROTEIN NUCLEIC ACID COMPLEXES IN NUCLEAR IMPORT

核输入中蛋白质核酸复合物的结构特征

• 批准号: 6308937
• 负责人: VITALY H CITOVSKY
• 金额: $ 0.97万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308937
• 关键词: bacteria; biomedical resource; genetics; growth /development; nucleic acids; plants; proteins

Nuclear import of proteins and nucleic acids is central to many cellular processes and cell-pathogen interactions. Some bacteria and viruses encode specialized proteins that Mansport genomic, DNA and RNA molecules of the invading pathogen into the host cell nucleus. One of these is the VirE2 protein of Agrobacterium. Agrobactenwn elicits neoplastic growths on many plant species by taiisporting a single-stranded copy of the bacterial tranderred DNA (T-DNA) into the. plant cell nucleus. VirE2 alone appears to be sufficient to transport T-DNA into the host cell nucleus. Since the T-DNA is not sequence specific, the complex between any ssDNA and VirE2 is competent for active nuclear imporL M 13 ssDNA and VirE2 were incubated and examined in the STEM. Both freeze-dried and negatively stained specimens were prepared. The rather rigid uniform filaments were analyzed, including mass/length, radial density profile analyses, and modeling to fit the observed stained data. A model of a hollow phone-cord like coil, 12.6 nin in diameter, with a helical pitch of 4.4 nrn, and approximately one VirE2 per twenty bases, fits all the data. TMV movement protein is not part of the TMV virion but is reponsible for the ftwisport into the host. Complexes of the protein and the TMV RNA were examined in the STEK but the protein/RNA ratio was too low. Further work is planned

蛋白质和核酸的核输入是许多 细胞过程和细胞-病原体相互作用。 一些细菌和 病毒编码专门的蛋白质，Mansport基因组，DNA和RNA 入侵病原体的分子进入宿主细胞核。 之一 这是农杆菌的VirE 2蛋白。 农杆菌引发 肿瘤生长在许多植物物种通过taiisporting 单链拷贝的细菌转基因DNA（T-DNA）进入。 植物细胞核 单独的VirE 2似乎足以运输 T-DNA进入宿主细胞核 因为T-DNA不是序列 具体地说，任何ssDNA和VirE 2之间的复合物都能胜任 将活性核导入体LM 13 ssDNA和VirE 2孵育并检测 在STEM。 冷冻干燥和阴性染色标本均为 制备 分析了相当刚性的均匀细丝，包括 质量/长度，径向密度分布分析和建模，以适应 观察染色数据。 空心电话线线圈模型，12.6 直径为1.9 mm，螺距为4.4 mm， 每20个碱基一个VirE 2，符合所有数据。 TMV运动蛋白 不是TMV病毒体的一部分，但负责将病毒转移到 主持人 蛋白质和TMV RNA的复合物被检测， STEK，但蛋白质/RNA比例太低。 进一步的工作 计划