Writers and Erasers of Ubiquitin Moieties in Control of Cell-to-Cell Transport in Plants
控制植物细胞间运输的泛素部分的书写者和擦除者
基本信息
- 批准号:10593120
- 负责人:
- 金额:$ 39.88万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-03-16 至 2027-01-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAgrobacteriumBacteriaBiologyCarrier ProteinsCellsGene Expression RegulationGenesGenetic TransformationGoalsKnowledgeLaboratoriesLife Cycle StagesMammalsMolecularMovementNanotubesNucleic AcidsPathway interactionsPlant ModelPlant VirusesPlantsPlasmodesmataProteinsRegulationResearchRoleTransport ProcessUbiquitinViralcell motilityepigenetic regulationhistone modificationinnovationintercellular connectionmacromoleculepathogenplant virus movement proteinprogramsresponsetoolubiquitin isopeptidaseubiquitin-protein ligase
项目摘要
PROJECT SUMMARY
Perhaps one of the most intriguing, yet least studied, aspects of macromolecular
transport is traffic through intercellular connections. These connections are termed tunneling
nanotubes in mammals and plasmodesmata (Pd) in plants. Whereas both mammalian and plant
viruses utilize cell-to-cell transport pathways, the first such capability was identified for
movement proteins (MPs) of plant viruses, the transport of which via Pd represents a
conceptual paradigm for intercellular traffic of macromolecules. Whereas the main research
effort in the field, including our lab, has been focused on the mechanism of MP cell-to-cell
movement, the important question of how the movement mechanism is controlled remained
largely unaddressed. The proposed research program aims to begin filing this gap in our
knowledge. Research in my laboratory focuses on three diverse aspects of plant biology and
plant-pathogen interactions: (i) cell-to-cell transport of the movement protein (MP) of the model
plant virus TMV; (ii) bacterium-to-plant cell transport of proteins and nucleic acids during genetic
transformation by Agrobacterium; and (iii) epigenetic regulation of gene expression, including
the pathogen response genes, by histone modifications. Recently, all three projects converged
on the regulatory roles of ubiquitin writers (E3 ligases) and erasers (deubiquitinases) in the plant
life cycle in general, and in the MP cell-to-cell transport in particular. Specifically, our findings
suggest that ubiquitin writers and erasers dynamically control two major aspects of the MP cell-
to-cell transport process: cargo stability/availability and assembly/disassembly of the Pd
transport machinery. The proposed program leverages the wealth of molecular tools and
experimental approaches that we have developed along the years to study cell-to-cell transport
of plant viruses and roles of protein ubiquitylation/deubiquitylation in plant-pathogen interactions
to address a new (and, so far, completely unresearched) level of regulation of intercellular
transport through Pd by reversible action of ubiquitin writers and erasers of the plant cell. We
believe that this goal is innovative and likely to be transformative also for studies of transport of
other proteins, viral and plant, through Pd.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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VITALY H CITOVSKY其他文献
VITALY H CITOVSKY的其他文献
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{{ truncateString('VITALY H CITOVSKY', 18)}}的其他基金
Equipment Supplement for R35 GM144059 "Writers and Erasers of Ubiquitin Moieties in Control of Cell-to-Cell Transport in Plants"
R35 GM144059 的设备补充材料“控制植物细胞间运输的泛素部分的写入器和擦除器”
- 批准号:
10796474 - 财政年份:2022
- 资助金额:
$ 39.88万 - 项目类别:
Writers and Erasers of Ubiquitin Moieties in Control of Cell-to-Cell Transport in Plants
控制植物细胞间运输的泛素部分的书写者和擦除者
- 批准号:
10328387 - 财政年份:2022
- 资助金额:
$ 39.88万 - 项目类别:
Control of Macromolecular Traffic Through Plasmodesmata
通过胞间连丝控制大分子交通
- 批准号:
8662922 - 财政年份:2013
- 资助金额:
$ 39.88万 - 项目类别:
Regulation of Macromolecular Transport Through Plasmodesmata
通过胞间连丝调节大分子运输
- 批准号:
7923558 - 财政年份:2009
- 资助金额:
$ 39.88万 - 项目类别:
STRUCT CHAR OF PROTEIN NUCLEIC ACID COMPLEXES IN NUCLEAR IMPORT
核输入中蛋白质核酸复合物的结构特征
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6444689 - 财政年份:2001
- 资助金额:
$ 39.88万 - 项目类别:
REGULATION OF PROTEIN TOBACCO MOSAIC VIRUS RNA COMPLEXES
蛋白质烟草花叶病毒 RNA 复合物的调控
- 批准号:
6053614 - 财政年份:2000
- 资助金额:
$ 39.88万 - 项目类别:
REGULATION OF PROTEIN TOBACCO MOSAIC VIRUS RNA COMPLEXES
蛋白质烟草花叶病毒 RNA 复合物的调控
- 批准号:
6499509 - 财政年份:2000
- 资助金额:
$ 39.88万 - 项目类别:
STRUCT CHAR OF PROTEIN NUCLEIC ACID COMPLEXES IN NUCLEAR IMPORT
核输入中蛋白质核酸复合物的结构特征
- 批准号:
6308937 - 财政年份:2000
- 资助金额:
$ 39.88万 - 项目类别:
REGULATION OF PROTEIN TOBACCO MOSAIC VIRUS RNA COMPLEXES
蛋白质烟草花叶病毒 RNA 复合物的调控
- 批准号:
6351922 - 财政年份:2000
- 资助金额:
$ 39.88万 - 项目类别:
STRUCTURE OF PLASMODESMATA (INTERCELLULAR PLANT JUNCTIONS)
Plasmodemata(植物细胞间连接)的结构
- 批准号:
6121818 - 财政年份:1999
- 资助金额:
$ 39.88万 - 项目类别:
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