权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Causes and consequences of regulatory genetic variation

调节性遗传变异的原因和后果

基本信息

批准号：
10793087
负责人：
Frank Wolfgang Albert
金额：
$ 24.62万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-01 至 2027-07-31
项目状态：
未结题

项目摘要

Project Summary / Abstract Genetic variation among individuals shapes important phenotypes, including the risk for common human diseases. In particular, regulatory genetic variation causes inter-individual differences in gene expression. The resulting gene expression differences account for a substantial portion of variation in many genetically complex traits. In spite of the critical importance of regulatory variation, many fundamental questions remain open. A particularly critical open question is that we only have a crude understanding of how the differences in gene expression that result from regulatory variation affect organismal phenotypes. This question is a key focus of the research program funded by the parent award for this equipment supplement. Under the parent award, research in my laboratory addresses these critical gaps in knowledge. Our work combines computational biology, quantitative and statistical genetics with experimental genome-wide approaches. We use the yeast Saccharomyces cerevisiae as a powerful and tractable model system for regulatory variation. This equipment supplement removes a critical roadblock in our research program. In our work, we construct libraries of thousands of strains. This award will support the purchase of a high-throughput phenotyping platform that will enable us to rigorously collect trait data from these strains. These data will allow us link genotype and phenotype rapidly and with quantitative precision. The requested instrument is in service of our long-term vision to improve our understanding of regulatory variation to the point at which it becomes possible to accurately predict the consequences of the DNA variants in an individual’s genome. This ability will be valuable for fundamental research and personalized approaches for improving human health.

项目总结/摘要个体间的遗传变异塑造了重要的表型，包括普通人类的风险。疾病特别是，调节性遗传变异导致基因表达的个体间差异。由此产生的基因表达差异解释了许多遗传学上的变异的很大一部分。复杂的特征尽管监管变化至关重要，但许多基本问题仍然悬而未决。一一个特别关键的开放性问题是，我们对基因差异是如何产生的只有一个粗略的理解，由调节变异引起的表达影响生物体表型。这个问题是一个关键的焦点研究计划的资助，由家长奖，为这个设备补充。在家长奖下，我实验室的研究解决了这些知识上的关键差距。我们的工作将计算生物学、定量和统计遗传学与实验全基因组相结合，接近。我们使用酵母酿酒酵母作为一个强大的和易处理的模型系统，监管变化。这种设备补充消除了我们研究计划中的一个关键障碍。在我们的工作中，数千种菌株的文库。该奖项将支持购买高通量表型分析这个平台将使我们能够严格收集这些菌株的性状数据。这些数据可以让我们基因型和表型快速和定量精度。所要求的工具是为了实现我们的长期愿景，以提高我们对监管的理解。变异到可以准确预测DNA结果的程度。个体基因组中的变异这种能力将是有价值的基础研究和个性化改善人类健康的方法。