Design of the Glomerulus and bOwman cApsuLe on a chip (GOAL)
芯片上肾小球和鲍曼胶囊的设计(目标)
基本信息
- 批准号:10810038
- 负责人:
- 金额:$ 43.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-09-21 至 2025-07-31
- 项目状态:未结题
- 来源:
- 关键词:3-Dimensional3D PrintAblationAcuteAffectArchitectureBasement membraneBiological ModelsBiomedical EngineeringBlood capillariesBowman&aposs spaceCell AdhesionCell Culture SystemCell Differentiation processCell physiologyCellsChronic Kidney FailureCoculture TechniquesCollagen Type IVCollectionComplexCuesCytoskeletonDependenceDevelopmentDialysis procedureDiameterDifferentiation AntigensDimensionsDiseaseDisease modelDrug ScreeningElectron MicroscopyEndothelial CellsEndotheliumEnvironmentExtracellular MatrixExtracellular Matrix ProteinsFiltrationFoot ProcessFunctional disorderFundingGelGeneticGenetic ModelsGenetic TranscriptionGeometryGlomerular CapillaryGlomerular capsule structureGoalsIn VitroInjuryKidneyKnockout MiceLasersLiquid substanceMeasuresMediatingMicrofluidic MicrochipsMicrofluidicsModelingMoldsMorphologyMusNational Institute of Diabetes and Digestive and Kidney DiseasesNephrosisPatternPerfusionPermeabilityPersonsPhysiologicalPhysiologyPrintingProcessPropertyProteinsProteinuriaProteomicsPublic HealthPulsatile FlowPuromycin AminonucleosideRenal Replacement TherapyRenal corpuscle structureRenal glomerular diseaseRoleShapesSideSomanSpecialized Epithelial CellStructureStudy modelsSupporting CellSurfaceSystemTechniquesTechnologyTestingTherapeuticThickThinnessTime StudyTissuesToxicity TestsWaste ProductsWorkcapsulecell growthcell typedesignextracellularfluid flowglomerular basement membraneglomerular endotheliumglomerular filtrationhigh riskimprovedin vivoinnovationlight microscopymetermulti-photonnew therapeutic targetnext generationnovelpodocytepressureresponseslit diaphragmtechnology developmenttooltool development
项目摘要
Loss of normal glomerular organization and selective glomerular filtration is one of the key
causes of chronic kidney disease, which often leads to the life-long need for renal replacement
therapies such as dialysis. Recreating glomerular filtration process in the lab is a challenging
undertaking that requires reproducing the complex glomerular architecture and fluid flow
patterns and populating the system with the specialized glomerular cell types, including
podocytes and endothelial cells. In the proposed work we combined multiphoton laser ablation
with 3D printed microfluidic devices to create a microfluidic glomerular model that reproduces
the complex network of glomerular capillaries within the hollow space (Bowman’s space) of the
renal corpuscle capsule (Bowman’s capsule). The Glomerular-BOwman’s CapSule system on
the chip (GOAL-chip) will be populated with podocytes and endothelial cells and tested for the
ability to support cell growth and differentiation and selective filtration under the conditions of
physiologically relevant filtration pressure and fluid perfusion. In Aim 1, we will determine
whether introducing complex capillary topology and appropriate fluid flow rates promotes
podocyte differentiation and functionality. In Aim 2, we will use podocyte-endothelial cell
coculture within the GOAL-chip and model diseases such as genetic glomerular disorders and
acute podocyte injury.
正常肾小球组织和选择性肾小球滤过功能的丧失是
慢性肾脏疾病的原因,这往往导致终身需要肾脏替代品
治疗方法,如透析。在实验室中重建肾小球滤过过程是一项具有挑战性的工作。
需要复制复杂的肾小球结构和液体流动的任务
模式和填充系统与专门的肾小球细胞类型,包括
足细胞和内皮细胞。在拟议的工作中,我们结合多光子激光烧蚀
用3D打印的微流体设备来创建微流体肾小球模型,
肾小球毛细血管的复杂网络内的中空空间(鲍曼的空间)的
肾小体囊(Bowman囊)。肾小球-鲍曼囊系统
芯片(GOAL芯片)将填充足细胞和内皮细胞,并检测
在以下条件下支持细胞生长和分化以及选择性过滤的能力:
生理相关的滤过压和液体灌注。在目标1中,我们将确定
引入复杂的毛细管拓扑结构和适当的流体流速是否促进
足细胞分化和功能。在目标2中,我们将使用足细胞-内皮细胞
在GOAL-芯片和模型疾病如遗传性肾小球疾病内共培养,
急性足细胞损伤
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Mira Krendel其他文献
Mira Krendel的其他文献
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{{ truncateString('Mira Krendel', 18)}}的其他基金
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
10587345 - 财政年份:2023
- 资助金额:
$ 43.5万 - 项目类别:
Class I myosins regulate protrusive forces at the actin-membrane interface
I 类肌球蛋白调节肌动蛋白-膜界面的突出力
- 批准号:
10445354 - 财政年份:2021
- 资助金额:
$ 43.5万 - 项目类别:
Class I myosins regulate protrusive forces at the actin-membrane interface
I 类肌球蛋白调节肌动蛋白-膜界面的突出力
- 批准号:
10211206 - 财政年份:2021
- 资助金额:
$ 43.5万 - 项目类别:
Class I myosins regulate protrusive forces at the actin-membrane interface
I 类肌球蛋白调节肌动蛋白-膜界面的突出力
- 批准号:
10582145 - 财政年份:2021
- 资助金额:
$ 43.5万 - 项目类别:
Class I myosins regulate protrusive forces at the actin-membrane interface
I 类肌球蛋白调节肌动蛋白-膜界面的突出力
- 批准号:
10612046 - 财政年份:2021
- 资助金额:
$ 43.5万 - 项目类别:
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
9176093 - 财政年份:2011
- 资助金额:
$ 43.5万 - 项目类别:
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
8188190 - 财政年份:2011
- 资助金额:
$ 43.5万 - 项目类别:
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
8328733 - 财政年份:2011
- 资助金额:
$ 43.5万 - 项目类别:
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
8675846 - 财政年份:2011
- 资助金额:
$ 43.5万 - 项目类别:
Role of myosin 1e in podocyte biology and renal filtration
肌球蛋白 1e 在足细胞生物学和肾滤过中的作用
- 批准号:
8517103 - 财政年份:2011
- 资助金额:
$ 43.5万 - 项目类别:
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